Role of secreted conjunctival mucosal cytokine and chemokine proteins in different stages of trachomatous disease.
Chlamydia trachomatis is responsible for trachoma, the primary cause of preventable blindness worldwide. Plans to eradicate trachoma using the World Health Organization's SAFE program (Surgery, Antibiotics, Facial Cleanliness and Environment Improvement) have resulted in recurrence of infection...
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ftdoajarticles:oai:doaj.org/article:5ce9e7a55f2b4d0a9ff855975cb04bfd 2023-05-15T15:16:10+02:00 Role of secreted conjunctival mucosal cytokine and chemokine proteins in different stages of trachomatous disease. Troy A Skwor Berna Atik Raj Prasad Kandel Him Kant Adhikari Bassant Sharma Deborah Dean 2008-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000264 https://doaj.org/article/5ce9e7a55f2b4d0a9ff855975cb04bfd EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2442224?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000264 https://doaj.org/article/5ce9e7a55f2b4d0a9ff855975cb04bfd PLoS Neglected Tropical Diseases, Vol 2, Iss 7, p e264 (2008) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2008 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000264 2022-12-31T01:09:27Z Chlamydia trachomatis is responsible for trachoma, the primary cause of preventable blindness worldwide. Plans to eradicate trachoma using the World Health Organization's SAFE program (Surgery, Antibiotics, Facial Cleanliness and Environment Improvement) have resulted in recurrence of infection and disease following cessation of treatment in many endemic countries, suggesting the need for a vaccine to control infection and trachomatous disease. Vaccine development requires, in part, knowledge of the mucosal host immune responses in both healthy and trachomatous conjuctivae-an area of research that remains insufficiently studied.We characterized 25 secreted cytokines and chemokines from the conjunctival mucosa of individuals residing in a trachoma endemic region of Nepal using Luminex X100 multiplexing technology. Immunomodulating effects of concurrent C. trachomatis infection were also examined. We found that proinflammatory cytokines IL-1beta (r = 0.259, P = 0.001) and TNFalpha (r = 0.168, P<0.05) were significantly associated with trachomatous disease and concurrent C. trachomatis infection compared with age and sex matched controls from the same region who did not have trachoma. In support of these findings, anti-inflammatory cytokine IL-1 receptor antagonist (IL-1Ra) was negatively associated with chronic scarring trachoma (r = -0.249, P = 0.001). Additional cytokines (Th1, IL-12p40 [r = -0.212, P<0.01], and Th2, IL-4 and IL-13 [r = -0.165 and -0.189, respectively, P<0.05 for both]) were negatively associated with chronic scarring trachoma, suggesting a protective role. Conversely, a pathogenic role for the Th3/Tr1 cytokine IL-10 (r = 0.180, P<0.05) was evident with increased levels for all trachoma grades. New risk factors for chronic scarring trachoma included IL-6 and IL-15 (r = 0.259 and 0.292, respectively, P<0.005 for both) with increased levels for concurrent C. trachomatis infections (r = 0.206, P<0.05, and r = 0.304, P<0.005, respectively). Chemokine protein levels for CCL11 ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 2 7 e264 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Troy A Skwor Berna Atik Raj Prasad Kandel Him Kant Adhikari Bassant Sharma Deborah Dean Role of secreted conjunctival mucosal cytokine and chemokine proteins in different stages of trachomatous disease. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Chlamydia trachomatis is responsible for trachoma, the primary cause of preventable blindness worldwide. Plans to eradicate trachoma using the World Health Organization's SAFE program (Surgery, Antibiotics, Facial Cleanliness and Environment Improvement) have resulted in recurrence of infection and disease following cessation of treatment in many endemic countries, suggesting the need for a vaccine to control infection and trachomatous disease. Vaccine development requires, in part, knowledge of the mucosal host immune responses in both healthy and trachomatous conjuctivae-an area of research that remains insufficiently studied.We characterized 25 secreted cytokines and chemokines from the conjunctival mucosa of individuals residing in a trachoma endemic region of Nepal using Luminex X100 multiplexing technology. Immunomodulating effects of concurrent C. trachomatis infection were also examined. We found that proinflammatory cytokines IL-1beta (r = 0.259, P = 0.001) and TNFalpha (r = 0.168, P<0.05) were significantly associated with trachomatous disease and concurrent C. trachomatis infection compared with age and sex matched controls from the same region who did not have trachoma. In support of these findings, anti-inflammatory cytokine IL-1 receptor antagonist (IL-1Ra) was negatively associated with chronic scarring trachoma (r = -0.249, P = 0.001). Additional cytokines (Th1, IL-12p40 [r = -0.212, P<0.01], and Th2, IL-4 and IL-13 [r = -0.165 and -0.189, respectively, P<0.05 for both]) were negatively associated with chronic scarring trachoma, suggesting a protective role. Conversely, a pathogenic role for the Th3/Tr1 cytokine IL-10 (r = 0.180, P<0.05) was evident with increased levels for all trachoma grades. New risk factors for chronic scarring trachoma included IL-6 and IL-15 (r = 0.259 and 0.292, respectively, P<0.005 for both) with increased levels for concurrent C. trachomatis infections (r = 0.206, P<0.05, and r = 0.304, P<0.005, respectively). Chemokine protein levels for CCL11 ... |
format |
Article in Journal/Newspaper |
author |
Troy A Skwor Berna Atik Raj Prasad Kandel Him Kant Adhikari Bassant Sharma Deborah Dean |
author_facet |
Troy A Skwor Berna Atik Raj Prasad Kandel Him Kant Adhikari Bassant Sharma Deborah Dean |
author_sort |
Troy A Skwor |
title |
Role of secreted conjunctival mucosal cytokine and chemokine proteins in different stages of trachomatous disease. |
title_short |
Role of secreted conjunctival mucosal cytokine and chemokine proteins in different stages of trachomatous disease. |
title_full |
Role of secreted conjunctival mucosal cytokine and chemokine proteins in different stages of trachomatous disease. |
title_fullStr |
Role of secreted conjunctival mucosal cytokine and chemokine proteins in different stages of trachomatous disease. |
title_full_unstemmed |
Role of secreted conjunctival mucosal cytokine and chemokine proteins in different stages of trachomatous disease. |
title_sort |
role of secreted conjunctival mucosal cytokine and chemokine proteins in different stages of trachomatous disease. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2008 |
url |
https://doi.org/10.1371/journal.pntd.0000264 https://doaj.org/article/5ce9e7a55f2b4d0a9ff855975cb04bfd |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 2, Iss 7, p e264 (2008) |
op_relation |
http://europepmc.org/articles/PMC2442224?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000264 https://doaj.org/article/5ce9e7a55f2b4d0a9ff855975cb04bfd |
op_doi |
https://doi.org/10.1371/journal.pntd.0000264 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
2 |
container_issue |
7 |
container_start_page |
e264 |
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1766346464738410496 |