Evaluation of serum procathepsin B, cystatin B and cystatin C as possible biomarkers of ovarian cancer
Objectives. To evaluate procathepsin B, as well as endogenous inhibitors of cysteine proteases (cystatin B and cystatin C) in biological fluids as possible biomarkers of ovarian cancer. To observe levels of serum procathepsin B in different age groups. Study design. The sample (N=27) of women with g...
Published in: | International Journal of Circumpolar Health |
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Taylor & Francis Group
2013
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Online Access: | https://doi.org/10.3402/ijch.v72i0.21215 https://doaj.org/article/5cd0830ef6db4db58555466c66a9d5db |
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ftdoajarticles:oai:doaj.org/article:5cd0830ef6db4db58555466c66a9d5db 2023-05-15T15:17:43+02:00 Evaluation of serum procathepsin B, cystatin B and cystatin C as possible biomarkers of ovarian cancer Elena A. Gashenko Valentina A. Lebedeva Ivan V. Brak Elena A. Tsykalenko Galina V. Vinokurova Tatyana A. Korolenko 2013-08-01T00:00:00Z https://doi.org/10.3402/ijch.v72i0.21215 https://doaj.org/article/5cd0830ef6db4db58555466c66a9d5db EN eng Taylor & Francis Group http://www.circumpolarhealthjournal.net/index.php/ijch/article/download/21215/pdf_1 https://doaj.org/toc/2242-3982 doi:10.3402/ijch.v72i0.21215 2242-3982 https://doaj.org/article/5cd0830ef6db4db58555466c66a9d5db International Journal of Circumpolar Health, Vol 72, Iss 0, Pp 1-5 (2013) ovarian cancer procathepsin B cystatin B cystatin C biomarkers Arctic medicine. Tropical medicine RC955-962 article 2013 ftdoajarticles https://doi.org/10.3402/ijch.v72i0.21215 2022-12-31T01:40:13Z Objectives. To evaluate procathepsin B, as well as endogenous inhibitors of cysteine proteases (cystatin B and cystatin C) in biological fluids as possible biomarkers of ovarian cancer. To observe levels of serum procathepsin B in different age groups. Study design. The sample (N=27) of women with gynaecological tumours included 18 patients with ovarian cancer (n=18) and 9 patients with benign ovarian tumours (n=9); 72 healthy women were in the control group. All patients were treated in Novosibirsk Regional Oncological Center, Russia. Serum samples of healthy women (n=40) aged 18–70 years were used as controls for common biomarker of ovarian cancer CA-125. In the Procathepsin B study, serum samples of healthy women (n=32) aged 18–40 years (n=14), 41–55 years (n=10) and 56–80 (n=8) years were used as controls. Methods. Common biomarker of ovarian cancer, CA-125, was assayed by using a commercial kit (Vector, Koltsovo, Novosibirsk Region, Russia). Procathepsin B was measured by means of a commercial kit for human procathepsin B (R&D, USA); cystatin C was measured by commercial ELISA kits for human (BioVendor, Czechia); cystatin B was measured by ELISA kits for human (USCN Life Science Inc., Wuhan, China). Statistical analysis was performed by one-way ANOVA (Statistica 10 Program). Results. In the control group, serum procathepsin B concentration did not reveal age dependency. In the ovarian cancer group, both levels of serum procathepsin B and standard biomarker CA-125 increased significantly (both p<0.001) compared with the control group. In the benign ovarian tumour group, serum procathepsin B (p<0.001) and CA-125 (p=0.004) increased about 2.5- and 8-fold compared to the control group. Serum cystatin B level increased up to 1.7-fold in the ovarian cancer group compared to the control group. The increase of serum CA-125 was about 3.5-fold higher (p=0.017) and procathepsin B was 1.8-fold higher (p<0.05) in the ovarian cancer ... Article in Journal/Newspaper Arctic Circumpolar Health International Journal of Circumpolar Health Directory of Open Access Journals: DOAJ Articles Arctic International Journal of Circumpolar Health 72 1 21215 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
ovarian cancer procathepsin B cystatin B cystatin C biomarkers Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
ovarian cancer procathepsin B cystatin B cystatin C biomarkers Arctic medicine. Tropical medicine RC955-962 Elena A. Gashenko Valentina A. Lebedeva Ivan V. Brak Elena A. Tsykalenko Galina V. Vinokurova Tatyana A. Korolenko Evaluation of serum procathepsin B, cystatin B and cystatin C as possible biomarkers of ovarian cancer |
topic_facet |
ovarian cancer procathepsin B cystatin B cystatin C biomarkers Arctic medicine. Tropical medicine RC955-962 |
description |
Objectives. To evaluate procathepsin B, as well as endogenous inhibitors of cysteine proteases (cystatin B and cystatin C) in biological fluids as possible biomarkers of ovarian cancer. To observe levels of serum procathepsin B in different age groups. Study design. The sample (N=27) of women with gynaecological tumours included 18 patients with ovarian cancer (n=18) and 9 patients with benign ovarian tumours (n=9); 72 healthy women were in the control group. All patients were treated in Novosibirsk Regional Oncological Center, Russia. Serum samples of healthy women (n=40) aged 18–70 years were used as controls for common biomarker of ovarian cancer CA-125. In the Procathepsin B study, serum samples of healthy women (n=32) aged 18–40 years (n=14), 41–55 years (n=10) and 56–80 (n=8) years were used as controls. Methods. Common biomarker of ovarian cancer, CA-125, was assayed by using a commercial kit (Vector, Koltsovo, Novosibirsk Region, Russia). Procathepsin B was measured by means of a commercial kit for human procathepsin B (R&D, USA); cystatin C was measured by commercial ELISA kits for human (BioVendor, Czechia); cystatin B was measured by ELISA kits for human (USCN Life Science Inc., Wuhan, China). Statistical analysis was performed by one-way ANOVA (Statistica 10 Program). Results. In the control group, serum procathepsin B concentration did not reveal age dependency. In the ovarian cancer group, both levels of serum procathepsin B and standard biomarker CA-125 increased significantly (both p<0.001) compared with the control group. In the benign ovarian tumour group, serum procathepsin B (p<0.001) and CA-125 (p=0.004) increased about 2.5- and 8-fold compared to the control group. Serum cystatin B level increased up to 1.7-fold in the ovarian cancer group compared to the control group. The increase of serum CA-125 was about 3.5-fold higher (p=0.017) and procathepsin B was 1.8-fold higher (p<0.05) in the ovarian cancer ... |
format |
Article in Journal/Newspaper |
author |
Elena A. Gashenko Valentina A. Lebedeva Ivan V. Brak Elena A. Tsykalenko Galina V. Vinokurova Tatyana A. Korolenko |
author_facet |
Elena A. Gashenko Valentina A. Lebedeva Ivan V. Brak Elena A. Tsykalenko Galina V. Vinokurova Tatyana A. Korolenko |
author_sort |
Elena A. Gashenko |
title |
Evaluation of serum procathepsin B, cystatin B and cystatin C as possible biomarkers of ovarian cancer |
title_short |
Evaluation of serum procathepsin B, cystatin B and cystatin C as possible biomarkers of ovarian cancer |
title_full |
Evaluation of serum procathepsin B, cystatin B and cystatin C as possible biomarkers of ovarian cancer |
title_fullStr |
Evaluation of serum procathepsin B, cystatin B and cystatin C as possible biomarkers of ovarian cancer |
title_full_unstemmed |
Evaluation of serum procathepsin B, cystatin B and cystatin C as possible biomarkers of ovarian cancer |
title_sort |
evaluation of serum procathepsin b, cystatin b and cystatin c as possible biomarkers of ovarian cancer |
publisher |
Taylor & Francis Group |
publishDate |
2013 |
url |
https://doi.org/10.3402/ijch.v72i0.21215 https://doaj.org/article/5cd0830ef6db4db58555466c66a9d5db |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Circumpolar Health International Journal of Circumpolar Health |
genre_facet |
Arctic Circumpolar Health International Journal of Circumpolar Health |
op_source |
International Journal of Circumpolar Health, Vol 72, Iss 0, Pp 1-5 (2013) |
op_relation |
http://www.circumpolarhealthjournal.net/index.php/ijch/article/download/21215/pdf_1 https://doaj.org/toc/2242-3982 doi:10.3402/ijch.v72i0.21215 2242-3982 https://doaj.org/article/5cd0830ef6db4db58555466c66a9d5db |
op_doi |
https://doi.org/10.3402/ijch.v72i0.21215 |
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International Journal of Circumpolar Health |
container_volume |
72 |
container_issue |
1 |
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21215 |
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1766347951034073088 |