Yellow Fever 17DD Vaccine Virus Infection Causes Detectable Changes in Chicken Embryos.
The yellow fever (YF) 17D vaccine is one of the most effective human vaccines ever created. The YF vaccine has been produced since 1937 in embryonated chicken eggs inoculated with the YF 17D virus. Yet, little information is available about the infection mechanism of YF 17DD virus in this biological...
Published in: | PLOS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2015
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0004064 https://doaj.org/article/5cc9dbb5de1f43dfa7e18d9ce8725c60 |
id |
ftdoajarticles:oai:doaj.org/article:5cc9dbb5de1f43dfa7e18d9ce8725c60 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:5cc9dbb5de1f43dfa7e18d9ce8725c60 2023-05-15T15:06:21+02:00 Yellow Fever 17DD Vaccine Virus Infection Causes Detectable Changes in Chicken Embryos. Pedro Paulo de Abreu Manso Barbara C E P Dias de Oliveira Patrícia Carvalho de Sequeira Yuli Rodrigues Maia de Souza Jessica Maria dos Santos Ferro Igor José da Silva Luzia Fátima Gonçalves Caputo Priscila Tavares Guedes Alexandre Araujo Cunha dos Santos Marcos da Silva Freire Myrna Cristina Bonaldo Marcelo Pelajo-Machado 2015-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004064 https://doaj.org/article/5cc9dbb5de1f43dfa7e18d9ce8725c60 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4570825?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004064 https://doaj.org/article/5cc9dbb5de1f43dfa7e18d9ce8725c60 PLoS Neglected Tropical Diseases, Vol 9, Iss 9, p e0004064 (2015) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2015 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004064 2022-12-31T11:43:03Z The yellow fever (YF) 17D vaccine is one of the most effective human vaccines ever created. The YF vaccine has been produced since 1937 in embryonated chicken eggs inoculated with the YF 17D virus. Yet, little information is available about the infection mechanism of YF 17DD virus in this biological model. To better understand this mechanism, we infected embryos of Gallus gallus domesticus and analyzed their histopathology after 72 hours of YF infection. Some embryos showed few apoptotic bodies in infected tissues, suggesting mild focal infection processes. Confocal and super-resolution microscopic analysis allowed us to identify as targets of viral infection: skeletal muscle cells, cardiomyocytes, nervous system cells, renal tubular epithelium, lung parenchyma, and fibroblasts associated with connective tissue in the perichondrium and dermis. The virus replication was heaviest in muscle tissues. In all of these specimens, RT-PCR methods confirmed the presence of replicative intermediate and genomic YF RNA. This clearer characterization of cell targets in chicken embryos paves the way for future development of a new YF vaccine based on a new cell culture system. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 9 9 e0004064 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Pedro Paulo de Abreu Manso Barbara C E P Dias de Oliveira Patrícia Carvalho de Sequeira Yuli Rodrigues Maia de Souza Jessica Maria dos Santos Ferro Igor José da Silva Luzia Fátima Gonçalves Caputo Priscila Tavares Guedes Alexandre Araujo Cunha dos Santos Marcos da Silva Freire Myrna Cristina Bonaldo Marcelo Pelajo-Machado Yellow Fever 17DD Vaccine Virus Infection Causes Detectable Changes in Chicken Embryos. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The yellow fever (YF) 17D vaccine is one of the most effective human vaccines ever created. The YF vaccine has been produced since 1937 in embryonated chicken eggs inoculated with the YF 17D virus. Yet, little information is available about the infection mechanism of YF 17DD virus in this biological model. To better understand this mechanism, we infected embryos of Gallus gallus domesticus and analyzed their histopathology after 72 hours of YF infection. Some embryos showed few apoptotic bodies in infected tissues, suggesting mild focal infection processes. Confocal and super-resolution microscopic analysis allowed us to identify as targets of viral infection: skeletal muscle cells, cardiomyocytes, nervous system cells, renal tubular epithelium, lung parenchyma, and fibroblasts associated with connective tissue in the perichondrium and dermis. The virus replication was heaviest in muscle tissues. In all of these specimens, RT-PCR methods confirmed the presence of replicative intermediate and genomic YF RNA. This clearer characterization of cell targets in chicken embryos paves the way for future development of a new YF vaccine based on a new cell culture system. |
format |
Article in Journal/Newspaper |
author |
Pedro Paulo de Abreu Manso Barbara C E P Dias de Oliveira Patrícia Carvalho de Sequeira Yuli Rodrigues Maia de Souza Jessica Maria dos Santos Ferro Igor José da Silva Luzia Fátima Gonçalves Caputo Priscila Tavares Guedes Alexandre Araujo Cunha dos Santos Marcos da Silva Freire Myrna Cristina Bonaldo Marcelo Pelajo-Machado |
author_facet |
Pedro Paulo de Abreu Manso Barbara C E P Dias de Oliveira Patrícia Carvalho de Sequeira Yuli Rodrigues Maia de Souza Jessica Maria dos Santos Ferro Igor José da Silva Luzia Fátima Gonçalves Caputo Priscila Tavares Guedes Alexandre Araujo Cunha dos Santos Marcos da Silva Freire Myrna Cristina Bonaldo Marcelo Pelajo-Machado |
author_sort |
Pedro Paulo de Abreu Manso |
title |
Yellow Fever 17DD Vaccine Virus Infection Causes Detectable Changes in Chicken Embryos. |
title_short |
Yellow Fever 17DD Vaccine Virus Infection Causes Detectable Changes in Chicken Embryos. |
title_full |
Yellow Fever 17DD Vaccine Virus Infection Causes Detectable Changes in Chicken Embryos. |
title_fullStr |
Yellow Fever 17DD Vaccine Virus Infection Causes Detectable Changes in Chicken Embryos. |
title_full_unstemmed |
Yellow Fever 17DD Vaccine Virus Infection Causes Detectable Changes in Chicken Embryos. |
title_sort |
yellow fever 17dd vaccine virus infection causes detectable changes in chicken embryos. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pntd.0004064 https://doaj.org/article/5cc9dbb5de1f43dfa7e18d9ce8725c60 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 9, Iss 9, p e0004064 (2015) |
op_relation |
http://europepmc.org/articles/PMC4570825?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004064 https://doaj.org/article/5cc9dbb5de1f43dfa7e18d9ce8725c60 |
op_doi |
https://doi.org/10.1371/journal.pntd.0004064 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
9 |
container_issue |
9 |
container_start_page |
e0004064 |
_version_ |
1766337968885202944 |