Insecticide resistance status and mechanisms in Aedes aegypti populations from Senegal.
Aedes aegypti is the main epidemic vector of arboviruses in Africa. In Senegal, control activities are mainly limited to mitigation of epidemics, with limited information available for Ae. aegypti populations. A better understanding of the current Ae. aegypti susceptibility status to various insecti...
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ftdoajarticles:oai:doaj.org/article:5cba9f7f601b47ca9a846e81f100d548 2023-05-15T15:12:39+02:00 Insecticide resistance status and mechanisms in Aedes aegypti populations from Senegal. Ndeye Marie Sene Konstantinos Mavridis El Hadji Ndiaye Cheikh Tidiane Diagne Alioune Gaye El Hadji Malick Ngom Yamar Ba Diawo Diallo John Vontas Ibrahima Dia Mawlouth Diallo 2021-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009393 https://doaj.org/article/5cba9f7f601b47ca9a846e81f100d548 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009393 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009393 https://doaj.org/article/5cba9f7f601b47ca9a846e81f100d548 PLoS Neglected Tropical Diseases, Vol 15, Iss 5, p e0009393 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009393 2022-12-31T05:59:59Z Aedes aegypti is the main epidemic vector of arboviruses in Africa. In Senegal, control activities are mainly limited to mitigation of epidemics, with limited information available for Ae. aegypti populations. A better understanding of the current Ae. aegypti susceptibility status to various insecticides and relevant resistance mechanisms involved is needed for the implementation of effective vector control strategies. The present study focuses on the detection of insecticide resistance and reveals the related mechanisms in Ae. aegypti populations from Senegal. Bioassays were performed on Ae. aegypti adults from nine Senegalese localities (Matam, Louga, Barkedji, Ziguinchor, Mbour, Fatick, Dakar, Kédougou and Touba). Mosquitoes were exposed to four classes of insecticides using the standard WHO protocols. Resistance mechanisms were investigated by genotyping for pyrethroid target site resistance mutations (V1016G, V1016I, F1534C and S989P) and measuring gene expression levels of key detoxification genes (CYP6BB2, CYP9J26, CYP9J28, CYP9J32, CYP9M6, CCEae3a and GSTD4). All collected populations were resistant to DDT and carbamates except for the ones in Matam (Northern region). Resistance to permethrin was uniformly detected in mosquitoes from all areas. Except for Barkédji and Touba, all populations were characterized by a susceptibility to 0.75% Permethrin. Susceptibility to type II pyrethroids was detected only in the Southern regions (Kédougou and Ziguinchor). All mosquito populations were susceptible to 5% Malathion, but only Kédougou and Matam mosquitoes were susceptible to 0.8% Malathion. All populations were resistant to 0.05% Pirimiphos-methyl, whereas those from Louga, Mbour and Barkédji, also exhibited resistance to 1% Fenitrothion. None of the known target site pyrethroid resistance mutations was present in the mosquito samples included in the genotyping analysis (performed in > 1500 samples). In contrast, a remarkably high (20-70-fold) overexpression of major detoxification genes was observed, ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 5 e0009393 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Ndeye Marie Sene Konstantinos Mavridis El Hadji Ndiaye Cheikh Tidiane Diagne Alioune Gaye El Hadji Malick Ngom Yamar Ba Diawo Diallo John Vontas Ibrahima Dia Mawlouth Diallo Insecticide resistance status and mechanisms in Aedes aegypti populations from Senegal. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Aedes aegypti is the main epidemic vector of arboviruses in Africa. In Senegal, control activities are mainly limited to mitigation of epidemics, with limited information available for Ae. aegypti populations. A better understanding of the current Ae. aegypti susceptibility status to various insecticides and relevant resistance mechanisms involved is needed for the implementation of effective vector control strategies. The present study focuses on the detection of insecticide resistance and reveals the related mechanisms in Ae. aegypti populations from Senegal. Bioassays were performed on Ae. aegypti adults from nine Senegalese localities (Matam, Louga, Barkedji, Ziguinchor, Mbour, Fatick, Dakar, Kédougou and Touba). Mosquitoes were exposed to four classes of insecticides using the standard WHO protocols. Resistance mechanisms were investigated by genotyping for pyrethroid target site resistance mutations (V1016G, V1016I, F1534C and S989P) and measuring gene expression levels of key detoxification genes (CYP6BB2, CYP9J26, CYP9J28, CYP9J32, CYP9M6, CCEae3a and GSTD4). All collected populations were resistant to DDT and carbamates except for the ones in Matam (Northern region). Resistance to permethrin was uniformly detected in mosquitoes from all areas. Except for Barkédji and Touba, all populations were characterized by a susceptibility to 0.75% Permethrin. Susceptibility to type II pyrethroids was detected only in the Southern regions (Kédougou and Ziguinchor). All mosquito populations were susceptible to 5% Malathion, but only Kédougou and Matam mosquitoes were susceptible to 0.8% Malathion. All populations were resistant to 0.05% Pirimiphos-methyl, whereas those from Louga, Mbour and Barkédji, also exhibited resistance to 1% Fenitrothion. None of the known target site pyrethroid resistance mutations was present in the mosquito samples included in the genotyping analysis (performed in > 1500 samples). In contrast, a remarkably high (20-70-fold) overexpression of major detoxification genes was observed, ... |
format |
Article in Journal/Newspaper |
author |
Ndeye Marie Sene Konstantinos Mavridis El Hadji Ndiaye Cheikh Tidiane Diagne Alioune Gaye El Hadji Malick Ngom Yamar Ba Diawo Diallo John Vontas Ibrahima Dia Mawlouth Diallo |
author_facet |
Ndeye Marie Sene Konstantinos Mavridis El Hadji Ndiaye Cheikh Tidiane Diagne Alioune Gaye El Hadji Malick Ngom Yamar Ba Diawo Diallo John Vontas Ibrahima Dia Mawlouth Diallo |
author_sort |
Ndeye Marie Sene |
title |
Insecticide resistance status and mechanisms in Aedes aegypti populations from Senegal. |
title_short |
Insecticide resistance status and mechanisms in Aedes aegypti populations from Senegal. |
title_full |
Insecticide resistance status and mechanisms in Aedes aegypti populations from Senegal. |
title_fullStr |
Insecticide resistance status and mechanisms in Aedes aegypti populations from Senegal. |
title_full_unstemmed |
Insecticide resistance status and mechanisms in Aedes aegypti populations from Senegal. |
title_sort |
insecticide resistance status and mechanisms in aedes aegypti populations from senegal. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009393 https://doaj.org/article/5cba9f7f601b47ca9a846e81f100d548 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 5, p e0009393 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009393 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009393 https://doaj.org/article/5cba9f7f601b47ca9a846e81f100d548 |
op_doi |
https://doi.org/10.1371/journal.pntd.0009393 |
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PLOS Neglected Tropical Diseases |
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15 |
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5 |
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e0009393 |
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1766343313946836992 |