Repeatome Analyses and Satellite DNA Chromosome Patterns in Deschampsia sukatschewii , D. cespitosa , and D. antarctica (Poaceae)
Subpolar and polar ecotypes of Deschampsia sukatschewii (Popl.) Roshev, D. cespitosa (L.) P. Beauv, and D. antarctica E. Desv. are well adapted to stressful environmental conditions, which make them useful model plants for genetic research and breeding. For the first time, the comparative repeatome...
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2022
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ftdoajarticles:oai:doaj.org/article:5cb25fd96e014897a0c66412459f6fdb 2023-05-15T13:41:03+02:00 Repeatome Analyses and Satellite DNA Chromosome Patterns in Deschampsia sukatschewii , D. cespitosa , and D. antarctica (Poaceae) Alexandra V. Amosova Olga Yu. Yurkevich Nadezhda L. Bolsheva Tatiana E. Samatadze Svyatoslav A. Zoshchuk Olga V. Muravenko 2022-04-01T00:00:00Z https://doi.org/10.3390/genes13050762 https://doaj.org/article/5cb25fd96e014897a0c66412459f6fdb EN eng MDPI AG https://www.mdpi.com/2073-4425/13/5/762 https://doaj.org/toc/2073-4425 doi:10.3390/genes13050762 2073-4425 https://doaj.org/article/5cb25fd96e014897a0c66412459f6fdb Genes, Vol 13, Iss 762, p 762 (2022) high-throughput sequencing Deschampsia sukatschewii D. antarctica D. cespitosa repeatome chromosome Genetics QH426-470 article 2022 ftdoajarticles https://doi.org/10.3390/genes13050762 2022-12-30T21:50:45Z Subpolar and polar ecotypes of Deschampsia sukatschewii (Popl.) Roshev, D. cespitosa (L.) P. Beauv, and D. antarctica E. Desv. are well adapted to stressful environmental conditions, which make them useful model plants for genetic research and breeding. For the first time, the comparative repeatome analyses of subpolar and polar D. sukatschewii , D. cespitosa , and D. antarctica was performed using RepeatExplorer/TAREAN pipelines and FISH-based chromosomal mapping of the identified satellite DNA families (satDNAs). In the studied species, mobile genetic elements of class 1 made up the majority of their repetitive DNA; interspecific variations in the total amount of Ty3/Gypsy and Ty1/Copia retroelements, DNA transposons, ribosomal, and satellite DNA were revealed; 12–18 high confident and 7–9 low confident putative satDNAs were identified. According to BLAST, most D. sukatschewii satDNAs demonstrated sequence similarity with satDNAs of D. antarctica and D. cespitosa indicating their common origin. Chromosomal mapping of 45S rDNA, 5S rDNA, and satDNAs of D. sukatschewii allowed us to construct the species karyograms and detect new molecular chromosome markers important for Deschampsia species. Our findings confirmed that genomes of D. sukatschewii and D. cespitosa were more closely related compared to D. antarctica according to repeatome composition and patterns of satDNA chromosomal distribution. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Genes 13 5 762 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
high-throughput sequencing Deschampsia sukatschewii D. antarctica D. cespitosa repeatome chromosome Genetics QH426-470 |
spellingShingle |
high-throughput sequencing Deschampsia sukatschewii D. antarctica D. cespitosa repeatome chromosome Genetics QH426-470 Alexandra V. Amosova Olga Yu. Yurkevich Nadezhda L. Bolsheva Tatiana E. Samatadze Svyatoslav A. Zoshchuk Olga V. Muravenko Repeatome Analyses and Satellite DNA Chromosome Patterns in Deschampsia sukatschewii , D. cespitosa , and D. antarctica (Poaceae) |
topic_facet |
high-throughput sequencing Deschampsia sukatschewii D. antarctica D. cespitosa repeatome chromosome Genetics QH426-470 |
description |
Subpolar and polar ecotypes of Deschampsia sukatschewii (Popl.) Roshev, D. cespitosa (L.) P. Beauv, and D. antarctica E. Desv. are well adapted to stressful environmental conditions, which make them useful model plants for genetic research and breeding. For the first time, the comparative repeatome analyses of subpolar and polar D. sukatschewii , D. cespitosa , and D. antarctica was performed using RepeatExplorer/TAREAN pipelines and FISH-based chromosomal mapping of the identified satellite DNA families (satDNAs). In the studied species, mobile genetic elements of class 1 made up the majority of their repetitive DNA; interspecific variations in the total amount of Ty3/Gypsy and Ty1/Copia retroelements, DNA transposons, ribosomal, and satellite DNA were revealed; 12–18 high confident and 7–9 low confident putative satDNAs were identified. According to BLAST, most D. sukatschewii satDNAs demonstrated sequence similarity with satDNAs of D. antarctica and D. cespitosa indicating their common origin. Chromosomal mapping of 45S rDNA, 5S rDNA, and satDNAs of D. sukatschewii allowed us to construct the species karyograms and detect new molecular chromosome markers important for Deschampsia species. Our findings confirmed that genomes of D. sukatschewii and D. cespitosa were more closely related compared to D. antarctica according to repeatome composition and patterns of satDNA chromosomal distribution. |
format |
Article in Journal/Newspaper |
author |
Alexandra V. Amosova Olga Yu. Yurkevich Nadezhda L. Bolsheva Tatiana E. Samatadze Svyatoslav A. Zoshchuk Olga V. Muravenko |
author_facet |
Alexandra V. Amosova Olga Yu. Yurkevich Nadezhda L. Bolsheva Tatiana E. Samatadze Svyatoslav A. Zoshchuk Olga V. Muravenko |
author_sort |
Alexandra V. Amosova |
title |
Repeatome Analyses and Satellite DNA Chromosome Patterns in Deschampsia sukatschewii , D. cespitosa , and D. antarctica (Poaceae) |
title_short |
Repeatome Analyses and Satellite DNA Chromosome Patterns in Deschampsia sukatschewii , D. cespitosa , and D. antarctica (Poaceae) |
title_full |
Repeatome Analyses and Satellite DNA Chromosome Patterns in Deschampsia sukatschewii , D. cespitosa , and D. antarctica (Poaceae) |
title_fullStr |
Repeatome Analyses and Satellite DNA Chromosome Patterns in Deschampsia sukatschewii , D. cespitosa , and D. antarctica (Poaceae) |
title_full_unstemmed |
Repeatome Analyses and Satellite DNA Chromosome Patterns in Deschampsia sukatschewii , D. cespitosa , and D. antarctica (Poaceae) |
title_sort |
repeatome analyses and satellite dna chromosome patterns in deschampsia sukatschewii , d. cespitosa , and d. antarctica (poaceae) |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/genes13050762 https://doaj.org/article/5cb25fd96e014897a0c66412459f6fdb |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Genes, Vol 13, Iss 762, p 762 (2022) |
op_relation |
https://www.mdpi.com/2073-4425/13/5/762 https://doaj.org/toc/2073-4425 doi:10.3390/genes13050762 2073-4425 https://doaj.org/article/5cb25fd96e014897a0c66412459f6fdb |
op_doi |
https://doi.org/10.3390/genes13050762 |
container_title |
Genes |
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13 |
container_issue |
5 |
container_start_page |
762 |
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1766145196410535936 |