A DNA vaccine against chikungunya virus is protective in mice and induces neutralizing antibodies in mice and nonhuman primates.
Chikungunya virus (CHIKV) is an emerging mosquito-borne alphavirus indigenous to tropical Africa and Asia. Acute illness is characterized by fever, arthralgias, conjunctivitis, rash, and sometimes arthritis. Relatively little is known about the antigenic targets for immunity, and no licensed vaccine...
Published in: | PLoS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2011
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0000928 https://doaj.org/article/5c41fd311157407db58e69ca49dd0aa8 |
id |
ftdoajarticles:oai:doaj.org/article:5c41fd311157407db58e69ca49dd0aa8 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:5c41fd311157407db58e69ca49dd0aa8 2023-05-15T15:11:09+02:00 A DNA vaccine against chikungunya virus is protective in mice and induces neutralizing antibodies in mice and nonhuman primates. Karthik Mallilankaraman Devon J Shedlock Huihui Bao Omkar U Kawalekar Paolo Fagone Aarthi A Ramanathan Bernadette Ferraro Jennifer Stabenow Paluru Vijayachari Senthil G Sundaram Nagarajan Muruganandam Gopalsamy Sarangan Padma Srikanth Amir S Khan Mark G Lewis J Joseph Kim Niranjan Y Sardesai Karuppiah Muthumani David B Weiner 2011-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000928 https://doaj.org/article/5c41fd311157407db58e69ca49dd0aa8 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3019110?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000928 https://doaj.org/article/5c41fd311157407db58e69ca49dd0aa8 PLoS Neglected Tropical Diseases, Vol 5, Iss 1, p e928 (2011) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2011 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000928 2022-12-31T01:43:15Z Chikungunya virus (CHIKV) is an emerging mosquito-borne alphavirus indigenous to tropical Africa and Asia. Acute illness is characterized by fever, arthralgias, conjunctivitis, rash, and sometimes arthritis. Relatively little is known about the antigenic targets for immunity, and no licensed vaccines or therapeutics are currently available for the pathogen. While the Aedes aegypti mosquito is its primary vector, recent evidence suggests that other carriers can transmit CHIKV thus raising concerns about its spread outside of natural endemic areas to new countries including the U.S. and Europe. Considering the potential for pandemic spread, understanding the development of immunity is paramount to the development of effective counter measures against CHIKV. In this study, we isolated a new CHIKV virus from an acutely infected human patient and developed a defined viral challenge stock in mice that allowed us to study viral pathogenesis and develop a viral neutralization assay. We then constructed a synthetic DNA vaccine delivered by in vivo electroporation (EP) that expresses a component of the CHIKV envelope glycoprotein and used this model to evaluate its efficacy. Vaccination induced robust antigen-specific cellular and humoral immune responses, which individually were capable of providing protection against CHIKV challenge in mice. Furthermore, vaccine studies in rhesus macaques demonstrated induction of nAb responses, which mimicked those induced in convalescent human patient sera. These data suggest a protective role for nAb against CHIKV disease and support further study of envelope-based CHIKV DNA vaccines. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 5 1 e928 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Karthik Mallilankaraman Devon J Shedlock Huihui Bao Omkar U Kawalekar Paolo Fagone Aarthi A Ramanathan Bernadette Ferraro Jennifer Stabenow Paluru Vijayachari Senthil G Sundaram Nagarajan Muruganandam Gopalsamy Sarangan Padma Srikanth Amir S Khan Mark G Lewis J Joseph Kim Niranjan Y Sardesai Karuppiah Muthumani David B Weiner A DNA vaccine against chikungunya virus is protective in mice and induces neutralizing antibodies in mice and nonhuman primates. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Chikungunya virus (CHIKV) is an emerging mosquito-borne alphavirus indigenous to tropical Africa and Asia. Acute illness is characterized by fever, arthralgias, conjunctivitis, rash, and sometimes arthritis. Relatively little is known about the antigenic targets for immunity, and no licensed vaccines or therapeutics are currently available for the pathogen. While the Aedes aegypti mosquito is its primary vector, recent evidence suggests that other carriers can transmit CHIKV thus raising concerns about its spread outside of natural endemic areas to new countries including the U.S. and Europe. Considering the potential for pandemic spread, understanding the development of immunity is paramount to the development of effective counter measures against CHIKV. In this study, we isolated a new CHIKV virus from an acutely infected human patient and developed a defined viral challenge stock in mice that allowed us to study viral pathogenesis and develop a viral neutralization assay. We then constructed a synthetic DNA vaccine delivered by in vivo electroporation (EP) that expresses a component of the CHIKV envelope glycoprotein and used this model to evaluate its efficacy. Vaccination induced robust antigen-specific cellular and humoral immune responses, which individually were capable of providing protection against CHIKV challenge in mice. Furthermore, vaccine studies in rhesus macaques demonstrated induction of nAb responses, which mimicked those induced in convalescent human patient sera. These data suggest a protective role for nAb against CHIKV disease and support further study of envelope-based CHIKV DNA vaccines. |
format |
Article in Journal/Newspaper |
author |
Karthik Mallilankaraman Devon J Shedlock Huihui Bao Omkar U Kawalekar Paolo Fagone Aarthi A Ramanathan Bernadette Ferraro Jennifer Stabenow Paluru Vijayachari Senthil G Sundaram Nagarajan Muruganandam Gopalsamy Sarangan Padma Srikanth Amir S Khan Mark G Lewis J Joseph Kim Niranjan Y Sardesai Karuppiah Muthumani David B Weiner |
author_facet |
Karthik Mallilankaraman Devon J Shedlock Huihui Bao Omkar U Kawalekar Paolo Fagone Aarthi A Ramanathan Bernadette Ferraro Jennifer Stabenow Paluru Vijayachari Senthil G Sundaram Nagarajan Muruganandam Gopalsamy Sarangan Padma Srikanth Amir S Khan Mark G Lewis J Joseph Kim Niranjan Y Sardesai Karuppiah Muthumani David B Weiner |
author_sort |
Karthik Mallilankaraman |
title |
A DNA vaccine against chikungunya virus is protective in mice and induces neutralizing antibodies in mice and nonhuman primates. |
title_short |
A DNA vaccine against chikungunya virus is protective in mice and induces neutralizing antibodies in mice and nonhuman primates. |
title_full |
A DNA vaccine against chikungunya virus is protective in mice and induces neutralizing antibodies in mice and nonhuman primates. |
title_fullStr |
A DNA vaccine against chikungunya virus is protective in mice and induces neutralizing antibodies in mice and nonhuman primates. |
title_full_unstemmed |
A DNA vaccine against chikungunya virus is protective in mice and induces neutralizing antibodies in mice and nonhuman primates. |
title_sort |
dna vaccine against chikungunya virus is protective in mice and induces neutralizing antibodies in mice and nonhuman primates. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2011 |
url |
https://doi.org/10.1371/journal.pntd.0000928 https://doaj.org/article/5c41fd311157407db58e69ca49dd0aa8 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 5, Iss 1, p e928 (2011) |
op_relation |
http://europepmc.org/articles/PMC3019110?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000928 https://doaj.org/article/5c41fd311157407db58e69ca49dd0aa8 |
op_doi |
https://doi.org/10.1371/journal.pntd.0000928 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
5 |
container_issue |
1 |
container_start_page |
e928 |
_version_ |
1766342052217356288 |