Identifying thresholds for classifying moderate-to-heavy soil-transmitted helminth intensity infections for FECPAKG2, McMaster, Mini-FLOTAC and qPCR.
The World Health Organization (WHO) has defined moderate-to-heavy intensity (M&HI) infections with soil-transmitted helminths (Ascaris lumbricoides, Trichuris trichiura and the two hookworms, Ancylostoma duodenale and Necator americanus) based on specific values of eggs per gram of stool, as mea...
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2020
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Online Access: | https://doi.org/10.1371/journal.pntd.0008296 https://doaj.org/article/5c1af073759a43d7aa4c8f37541899a3 |
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ftdoajarticles:oai:doaj.org/article:5c1af073759a43d7aa4c8f37541899a3 2023-05-15T15:14:18+02:00 Identifying thresholds for classifying moderate-to-heavy soil-transmitted helminth intensity infections for FECPAKG2, McMaster, Mini-FLOTAC and qPCR. Bruno Levecke Piet Cools Marco Albonico Shaali Ame Cécile Angebault Mio Ayana Jerzy M Behnke Jeffrey M Bethony Giuseppe Cringoli Daniel Dana Bertrand Guillard Nguyen Thi Viet Hoa Gagandeep Kang Deepthi Kattula Jennifer Keiser Andrew C Kotze Leonardo F Matoso Maria P Maurelli James S McCarthy Zeleke Mekonnen Greg Mirams Antonio Montresor Rodrigo Corrêa Oliveira Maria V Periago Simone A Pinto Laura Rinaldi Somphou Sayasone Laurentine Sumo Louis-Albert Tchuem-Tchuenté Dang Thi Cam Thach Eurion Thomas Ahmed Zeynudin Jaco J Verweij Johnny Vlaminck Jozef Vercruysse 2020-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008296 https://doaj.org/article/5c1af073759a43d7aa4c8f37541899a3 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008296 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008296 https://doaj.org/article/5c1af073759a43d7aa4c8f37541899a3 PLoS Neglected Tropical Diseases, Vol 14, Iss 7, p e0008296 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008296 2022-12-31T13:50:09Z The World Health Organization (WHO) has defined moderate-to-heavy intensity (M&HI) infections with soil-transmitted helminths (Ascaris lumbricoides, Trichuris trichiura and the two hookworms, Ancylostoma duodenale and Necator americanus) based on specific values of eggs per gram of stool, as measured by the Kato-Katz method. There are a variety of novel microscopy and DNA-based methods but it remains unclear whether applying current WHO thresholds on to these methods allows for a reliable classification of M&HI infections. We evaluated both WHO and method-specific thresholds for classifying the M&HI infections for novel microscopic (FECPAKG2, McMaster and Mini-FLOTAC) and DNA-based (qPCR) diagnostic methods. For this, we determined method-specific thresholds that best classified M&HI infections (defined by Kato-Katz and WHO thresholds; reference method) in two multi-country drug efficacy studies. Subsequently, we verified whether applying these method-specific thresholds improved the agreement in classifying M&HI infections compared to the reference method. When we applied the WHO thresholds, the new microscopic methods mainly misclassified M&HI as low intensity, and to a lesser extent low intensity infection as M&HI. For FECPAKG2, applying the method-specific thresholds significantly improved the agreement for Ascaris (moderate → substantial), Trichuris and hookworms (fair → moderate). For Mini-FLOTAC, a significantly improved agreement was observed for hookworms only (fair → moderate). For the other STHs, the agreement was almost perfect and remained unchanged. For McMaster, the method-specific thresholds revealed a fair to a substantial agreement but did not significantly improve the agreement. For qPCR, the method-specific thresholds based on genome equivalents per ml of DNA moderately agreed with the reference method for hookworm and Trichuris infections. For Ascaris, there was a substantial agreement. We defined method-specific thresholds that improved the classification of ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 7 e0008296 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Bruno Levecke Piet Cools Marco Albonico Shaali Ame Cécile Angebault Mio Ayana Jerzy M Behnke Jeffrey M Bethony Giuseppe Cringoli Daniel Dana Bertrand Guillard Nguyen Thi Viet Hoa Gagandeep Kang Deepthi Kattula Jennifer Keiser Andrew C Kotze Leonardo F Matoso Maria P Maurelli James S McCarthy Zeleke Mekonnen Greg Mirams Antonio Montresor Rodrigo Corrêa Oliveira Maria V Periago Simone A Pinto Laura Rinaldi Somphou Sayasone Laurentine Sumo Louis-Albert Tchuem-Tchuenté Dang Thi Cam Thach Eurion Thomas Ahmed Zeynudin Jaco J Verweij Johnny Vlaminck Jozef Vercruysse Identifying thresholds for classifying moderate-to-heavy soil-transmitted helminth intensity infections for FECPAKG2, McMaster, Mini-FLOTAC and qPCR. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The World Health Organization (WHO) has defined moderate-to-heavy intensity (M&HI) infections with soil-transmitted helminths (Ascaris lumbricoides, Trichuris trichiura and the two hookworms, Ancylostoma duodenale and Necator americanus) based on specific values of eggs per gram of stool, as measured by the Kato-Katz method. There are a variety of novel microscopy and DNA-based methods but it remains unclear whether applying current WHO thresholds on to these methods allows for a reliable classification of M&HI infections. We evaluated both WHO and method-specific thresholds for classifying the M&HI infections for novel microscopic (FECPAKG2, McMaster and Mini-FLOTAC) and DNA-based (qPCR) diagnostic methods. For this, we determined method-specific thresholds that best classified M&HI infections (defined by Kato-Katz and WHO thresholds; reference method) in two multi-country drug efficacy studies. Subsequently, we verified whether applying these method-specific thresholds improved the agreement in classifying M&HI infections compared to the reference method. When we applied the WHO thresholds, the new microscopic methods mainly misclassified M&HI as low intensity, and to a lesser extent low intensity infection as M&HI. For FECPAKG2, applying the method-specific thresholds significantly improved the agreement for Ascaris (moderate → substantial), Trichuris and hookworms (fair → moderate). For Mini-FLOTAC, a significantly improved agreement was observed for hookworms only (fair → moderate). For the other STHs, the agreement was almost perfect and remained unchanged. For McMaster, the method-specific thresholds revealed a fair to a substantial agreement but did not significantly improve the agreement. For qPCR, the method-specific thresholds based on genome equivalents per ml of DNA moderately agreed with the reference method for hookworm and Trichuris infections. For Ascaris, there was a substantial agreement. We defined method-specific thresholds that improved the classification of ... |
format |
Article in Journal/Newspaper |
author |
Bruno Levecke Piet Cools Marco Albonico Shaali Ame Cécile Angebault Mio Ayana Jerzy M Behnke Jeffrey M Bethony Giuseppe Cringoli Daniel Dana Bertrand Guillard Nguyen Thi Viet Hoa Gagandeep Kang Deepthi Kattula Jennifer Keiser Andrew C Kotze Leonardo F Matoso Maria P Maurelli James S McCarthy Zeleke Mekonnen Greg Mirams Antonio Montresor Rodrigo Corrêa Oliveira Maria V Periago Simone A Pinto Laura Rinaldi Somphou Sayasone Laurentine Sumo Louis-Albert Tchuem-Tchuenté Dang Thi Cam Thach Eurion Thomas Ahmed Zeynudin Jaco J Verweij Johnny Vlaminck Jozef Vercruysse |
author_facet |
Bruno Levecke Piet Cools Marco Albonico Shaali Ame Cécile Angebault Mio Ayana Jerzy M Behnke Jeffrey M Bethony Giuseppe Cringoli Daniel Dana Bertrand Guillard Nguyen Thi Viet Hoa Gagandeep Kang Deepthi Kattula Jennifer Keiser Andrew C Kotze Leonardo F Matoso Maria P Maurelli James S McCarthy Zeleke Mekonnen Greg Mirams Antonio Montresor Rodrigo Corrêa Oliveira Maria V Periago Simone A Pinto Laura Rinaldi Somphou Sayasone Laurentine Sumo Louis-Albert Tchuem-Tchuenté Dang Thi Cam Thach Eurion Thomas Ahmed Zeynudin Jaco J Verweij Johnny Vlaminck Jozef Vercruysse |
author_sort |
Bruno Levecke |
title |
Identifying thresholds for classifying moderate-to-heavy soil-transmitted helminth intensity infections for FECPAKG2, McMaster, Mini-FLOTAC and qPCR. |
title_short |
Identifying thresholds for classifying moderate-to-heavy soil-transmitted helminth intensity infections for FECPAKG2, McMaster, Mini-FLOTAC and qPCR. |
title_full |
Identifying thresholds for classifying moderate-to-heavy soil-transmitted helminth intensity infections for FECPAKG2, McMaster, Mini-FLOTAC and qPCR. |
title_fullStr |
Identifying thresholds for classifying moderate-to-heavy soil-transmitted helminth intensity infections for FECPAKG2, McMaster, Mini-FLOTAC and qPCR. |
title_full_unstemmed |
Identifying thresholds for classifying moderate-to-heavy soil-transmitted helminth intensity infections for FECPAKG2, McMaster, Mini-FLOTAC and qPCR. |
title_sort |
identifying thresholds for classifying moderate-to-heavy soil-transmitted helminth intensity infections for fecpakg2, mcmaster, mini-flotac and qpcr. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008296 https://doaj.org/article/5c1af073759a43d7aa4c8f37541899a3 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 7, p e0008296 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008296 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008296 https://doaj.org/article/5c1af073759a43d7aa4c8f37541899a3 |
op_doi |
https://doi.org/10.1371/journal.pntd.0008296 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
7 |
container_start_page |
e0008296 |
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