Evaluation of imatinib mesylate (Gleevec) on KAI1/CD82 gene expression in breast cancer MCF-7 cells using quantitative real-time PCR
Objective: To evaluate the effect of imatinib mesylate on cell viability, anti cancer effect through modulation of KAI1/CD82 gene expression in breast cancer MCF-7 cell line. Methods: The effects of imatinib mesylate on cell viability in MCF-7 cell line were assessed using MTT assay and IC50 value w...
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2016
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Online Access: | https://doi.org/10.1016/j.apjtb.2015.10.006 https://doaj.org/article/5c12b9310e3a4e6894967b0c9be67de8 |
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ftdoajarticles:oai:doaj.org/article:5c12b9310e3a4e6894967b0c9be67de8 2023-05-15T15:06:32+02:00 Evaluation of imatinib mesylate (Gleevec) on KAI1/CD82 gene expression in breast cancer MCF-7 cells using quantitative real-time PCR Seyed Ataollah Sadat Shandiz Marjan Khosravani Sepideh Mohammadi Hassan Noorbazargan Amir Mirzaie Davoud Nouri Inanlou Mojgan Dalirsaber Jalali Hamidreza Jouzaghkar Fahimeh Baghbani-Arani Behta Keshavarz-Pakseresht 2016-02-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2015.10.006 https://doaj.org/article/5c12b9310e3a4e6894967b0c9be67de8 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115002567 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2015.10.006 https://doaj.org/article/5c12b9310e3a4e6894967b0c9be67de8 Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 2, Pp 159-163 (2016) Imatinib mesylate KAI1/CD82 Metastasis Breast cancer Real-time PCR Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2016 ftdoajarticles https://doi.org/10.1016/j.apjtb.2015.10.006 2022-12-31T02:10:49Z Objective: To evaluate the effect of imatinib mesylate on cell viability, anti cancer effect through modulation of KAI1/CD82 gene expression in breast cancer MCF-7 cell line. Methods: The effects of imatinib mesylate on cell viability in MCF-7 cell line were assessed using MTT assay and IC50 value was determined. GAPDH and KAI1/CD82 were selected as reference and target genes, respectively. Quantitative real time PCR technique was applied for investigation of KAI1/CD82 gene expression in human breast cancer MCF-7 cells. Subsequently, the quantity of KAI1 compared to GAPDH gene expressions were analyzed using the formula; 2−ΔΔCt. Results: Imatinib was showed to have a dose-dependent inhibitory effect on the viability of MCF-7 cells. CD82/GAPDH gene expression ratios were 1.322 ± 0.030 (P > 0.05), 2.052 ± 0.200 (P < 0.05), 2.151 ± 0.270 (P < 0.05) for 10, 20 and 40 μmol/L of imatinib concentrations. Conclusions: Based on the present data, imatinib mesylate might modulate metastasis by up-regulating KAI1/CD82 gene expression in human breast MCF-7 cancer cell line. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 6 2 159 163 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
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Imatinib mesylate KAI1/CD82 Metastasis Breast cancer Real-time PCR Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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Imatinib mesylate KAI1/CD82 Metastasis Breast cancer Real-time PCR Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Seyed Ataollah Sadat Shandiz Marjan Khosravani Sepideh Mohammadi Hassan Noorbazargan Amir Mirzaie Davoud Nouri Inanlou Mojgan Dalirsaber Jalali Hamidreza Jouzaghkar Fahimeh Baghbani-Arani Behta Keshavarz-Pakseresht Evaluation of imatinib mesylate (Gleevec) on KAI1/CD82 gene expression in breast cancer MCF-7 cells using quantitative real-time PCR |
topic_facet |
Imatinib mesylate KAI1/CD82 Metastasis Breast cancer Real-time PCR Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To evaluate the effect of imatinib mesylate on cell viability, anti cancer effect through modulation of KAI1/CD82 gene expression in breast cancer MCF-7 cell line. Methods: The effects of imatinib mesylate on cell viability in MCF-7 cell line were assessed using MTT assay and IC50 value was determined. GAPDH and KAI1/CD82 were selected as reference and target genes, respectively. Quantitative real time PCR technique was applied for investigation of KAI1/CD82 gene expression in human breast cancer MCF-7 cells. Subsequently, the quantity of KAI1 compared to GAPDH gene expressions were analyzed using the formula; 2−ΔΔCt. Results: Imatinib was showed to have a dose-dependent inhibitory effect on the viability of MCF-7 cells. CD82/GAPDH gene expression ratios were 1.322 ± 0.030 (P > 0.05), 2.052 ± 0.200 (P < 0.05), 2.151 ± 0.270 (P < 0.05) for 10, 20 and 40 μmol/L of imatinib concentrations. Conclusions: Based on the present data, imatinib mesylate might modulate metastasis by up-regulating KAI1/CD82 gene expression in human breast MCF-7 cancer cell line. |
format |
Article in Journal/Newspaper |
author |
Seyed Ataollah Sadat Shandiz Marjan Khosravani Sepideh Mohammadi Hassan Noorbazargan Amir Mirzaie Davoud Nouri Inanlou Mojgan Dalirsaber Jalali Hamidreza Jouzaghkar Fahimeh Baghbani-Arani Behta Keshavarz-Pakseresht |
author_facet |
Seyed Ataollah Sadat Shandiz Marjan Khosravani Sepideh Mohammadi Hassan Noorbazargan Amir Mirzaie Davoud Nouri Inanlou Mojgan Dalirsaber Jalali Hamidreza Jouzaghkar Fahimeh Baghbani-Arani Behta Keshavarz-Pakseresht |
author_sort |
Seyed Ataollah Sadat Shandiz |
title |
Evaluation of imatinib mesylate (Gleevec) on KAI1/CD82 gene expression in breast cancer MCF-7 cells using quantitative real-time PCR |
title_short |
Evaluation of imatinib mesylate (Gleevec) on KAI1/CD82 gene expression in breast cancer MCF-7 cells using quantitative real-time PCR |
title_full |
Evaluation of imatinib mesylate (Gleevec) on KAI1/CD82 gene expression in breast cancer MCF-7 cells using quantitative real-time PCR |
title_fullStr |
Evaluation of imatinib mesylate (Gleevec) on KAI1/CD82 gene expression in breast cancer MCF-7 cells using quantitative real-time PCR |
title_full_unstemmed |
Evaluation of imatinib mesylate (Gleevec) on KAI1/CD82 gene expression in breast cancer MCF-7 cells using quantitative real-time PCR |
title_sort |
evaluation of imatinib mesylate (gleevec) on kai1/cd82 gene expression in breast cancer mcf-7 cells using quantitative real-time pcr |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2016 |
url |
https://doi.org/10.1016/j.apjtb.2015.10.006 https://doaj.org/article/5c12b9310e3a4e6894967b0c9be67de8 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 2, Pp 159-163 (2016) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115002567 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2015.10.006 https://doaj.org/article/5c12b9310e3a4e6894967b0c9be67de8 |
op_doi |
https://doi.org/10.1016/j.apjtb.2015.10.006 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
6 |
container_issue |
2 |
container_start_page |
159 |
op_container_end_page |
163 |
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