Antihelminthic Activity of Lophira Lanceolata on Heligmosomoides polygyrus Using an Automated High-Throughput Method
Background. There are about 13 parasitic infections that are responsible for significant morbidity and mortality but have not received the attention they deserve; thus, they are now known as “neglected tropical diseases” (NTDs). This study was aimed at evaluating the antihelminthic activities of Lop...
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Hindawi Limited
2023
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Online Access: | https://doi.org/10.1155/2023/9504296 https://doaj.org/article/5c0e9c049c6840ae917121999859dba2 |
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ftdoajarticles:oai:doaj.org/article:5c0e9c049c6840ae917121999859dba2 2023-05-15T15:16:44+02:00 Antihelminthic Activity of Lophira Lanceolata on Heligmosomoides polygyrus Using an Automated High-Throughput Method Yamssi Cédric Noumedem Anangmo Christelle Nadia Simeni Njonnou Sylvain Raoul Samuel Berinyuy Mounvera Abdel Azizi Tientcheu Noutong Jemimah Sandra Ngouyamsa Nsapkain Aboubakar Sidiki Vincent Khan Payne 2023-01-01T00:00:00Z https://doi.org/10.1155/2023/9504296 https://doaj.org/article/5c0e9c049c6840ae917121999859dba2 EN eng Hindawi Limited http://dx.doi.org/10.1155/2023/9504296 https://doaj.org/toc/1687-9694 1687-9694 doi:10.1155/2023/9504296 https://doaj.org/article/5c0e9c049c6840ae917121999859dba2 Journal of Tropical Medicine, Vol 2023 (2023) Arctic medicine. Tropical medicine RC955-962 article 2023 ftdoajarticles https://doi.org/10.1155/2023/9504296 2023-02-05T01:33:47Z Background. There are about 13 parasitic infections that are responsible for significant morbidity and mortality but have not received the attention they deserve; thus, they are now known as “neglected tropical diseases” (NTDs). This study was aimed at evaluating the antihelminthic activities of Lophira lanceolata using an automated high-throughput method. Methods. The antihelminthic activity effect of the extracts against H. polygyrus was determined using an automated high-throughput method. For the egg-hatching test, 100 μL of embryonated egg suspension (60 eggs) was added to 100 μL of various concentrations of extracts, levamisole, and 1.5% DMSO in a 96-well round-bottom microtitre plate. After mixing, the 96-well microplate was placed in WMicroTracker and incubated for 24 h at 25°C; the movements were recorded every 30 minutes. The same procedure was used for the larval motility assays, where 100 μL of L1 or L2 larvae (50 larvae) were put in contact with 100 μL of various concentrations of extracts. Results. The ovicidal activity (hatching) had an IC50 of 1.4 mg/mL for the ethanol extract. The aqueous and ethanol extracts of L. lanceolata showed larvicidal activity on the L1 larvae with IC50 of 1.85 mg/mL and 2.4 mg/mL, respectively, as well as on the L2 larvae with IC50 values of 1.08 mg/mL and 1.02 mg/mL for the aqueous and ethanol extracts, respectively. These results showed that the aqueous extract exhibited a stronger inhibitory power on the hatching rate of parasites than ethanol extracts, while the contrary effect was observed for the larval motility assays. Conclusion. This study provides scientific data on the use of L. lanceolata by the local population for the treatment of helminthiases. However, in vivo and toxicity tests are necessary to assess its activity and safety. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Tropical Medicine 2023 1 7 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Yamssi Cédric Noumedem Anangmo Christelle Nadia Simeni Njonnou Sylvain Raoul Samuel Berinyuy Mounvera Abdel Azizi Tientcheu Noutong Jemimah Sandra Ngouyamsa Nsapkain Aboubakar Sidiki Vincent Khan Payne Antihelminthic Activity of Lophira Lanceolata on Heligmosomoides polygyrus Using an Automated High-Throughput Method |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 |
description |
Background. There are about 13 parasitic infections that are responsible for significant morbidity and mortality but have not received the attention they deserve; thus, they are now known as “neglected tropical diseases” (NTDs). This study was aimed at evaluating the antihelminthic activities of Lophira lanceolata using an automated high-throughput method. Methods. The antihelminthic activity effect of the extracts against H. polygyrus was determined using an automated high-throughput method. For the egg-hatching test, 100 μL of embryonated egg suspension (60 eggs) was added to 100 μL of various concentrations of extracts, levamisole, and 1.5% DMSO in a 96-well round-bottom microtitre plate. After mixing, the 96-well microplate was placed in WMicroTracker and incubated for 24 h at 25°C; the movements were recorded every 30 minutes. The same procedure was used for the larval motility assays, where 100 μL of L1 or L2 larvae (50 larvae) were put in contact with 100 μL of various concentrations of extracts. Results. The ovicidal activity (hatching) had an IC50 of 1.4 mg/mL for the ethanol extract. The aqueous and ethanol extracts of L. lanceolata showed larvicidal activity on the L1 larvae with IC50 of 1.85 mg/mL and 2.4 mg/mL, respectively, as well as on the L2 larvae with IC50 values of 1.08 mg/mL and 1.02 mg/mL for the aqueous and ethanol extracts, respectively. These results showed that the aqueous extract exhibited a stronger inhibitory power on the hatching rate of parasites than ethanol extracts, while the contrary effect was observed for the larval motility assays. Conclusion. This study provides scientific data on the use of L. lanceolata by the local population for the treatment of helminthiases. However, in vivo and toxicity tests are necessary to assess its activity and safety. |
format |
Article in Journal/Newspaper |
author |
Yamssi Cédric Noumedem Anangmo Christelle Nadia Simeni Njonnou Sylvain Raoul Samuel Berinyuy Mounvera Abdel Azizi Tientcheu Noutong Jemimah Sandra Ngouyamsa Nsapkain Aboubakar Sidiki Vincent Khan Payne |
author_facet |
Yamssi Cédric Noumedem Anangmo Christelle Nadia Simeni Njonnou Sylvain Raoul Samuel Berinyuy Mounvera Abdel Azizi Tientcheu Noutong Jemimah Sandra Ngouyamsa Nsapkain Aboubakar Sidiki Vincent Khan Payne |
author_sort |
Yamssi Cédric |
title |
Antihelminthic Activity of Lophira Lanceolata on Heligmosomoides polygyrus Using an Automated High-Throughput Method |
title_short |
Antihelminthic Activity of Lophira Lanceolata on Heligmosomoides polygyrus Using an Automated High-Throughput Method |
title_full |
Antihelminthic Activity of Lophira Lanceolata on Heligmosomoides polygyrus Using an Automated High-Throughput Method |
title_fullStr |
Antihelminthic Activity of Lophira Lanceolata on Heligmosomoides polygyrus Using an Automated High-Throughput Method |
title_full_unstemmed |
Antihelminthic Activity of Lophira Lanceolata on Heligmosomoides polygyrus Using an Automated High-Throughput Method |
title_sort |
antihelminthic activity of lophira lanceolata on heligmosomoides polygyrus using an automated high-throughput method |
publisher |
Hindawi Limited |
publishDate |
2023 |
url |
https://doi.org/10.1155/2023/9504296 https://doaj.org/article/5c0e9c049c6840ae917121999859dba2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Tropical Medicine, Vol 2023 (2023) |
op_relation |
http://dx.doi.org/10.1155/2023/9504296 https://doaj.org/toc/1687-9694 1687-9694 doi:10.1155/2023/9504296 https://doaj.org/article/5c0e9c049c6840ae917121999859dba2 |
op_doi |
https://doi.org/10.1155/2023/9504296 |
container_title |
Journal of Tropical Medicine |
container_volume |
2023 |
container_start_page |
1 |
op_container_end_page |
7 |
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1766347029468938240 |