Genetic diversity of Plasmodium vivax and Plasmodium falciparum lactate dehydrogenases in Myanmar isolates
Abstract Background Plasmodium lactate dehydrogenase (pLDH) is a major target in diagnosing the erythrocytic stage of malaria parasites because it is highly expressed during blood-stage parasites and is distinguished from human LDH. Rapid diagnostic tests (RDTs) for malaria use pLDH as a target anti...
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ftdoajarticles:oai:doaj.org/article:5c0816bd1a654b2a854d6fc3cf26cdea 2023-05-15T15:15:00+02:00 Genetic diversity of Plasmodium vivax and Plasmodium falciparum lactate dehydrogenases in Myanmar isolates Jinyoung Lee Tae Im Kim Hương Giang Lê Won Gi Yoo Jung-Mi Kang Seong-Kyu Ahn Moe Kyaw Myint Khin Lin Tong-Soo Kim Byoung-Kuk Na 2020-02-01T00:00:00Z https://doi.org/10.1186/s12936-020-3134-y https://doaj.org/article/5c0816bd1a654b2a854d6fc3cf26cdea EN eng BMC https://doi.org/10.1186/s12936-020-3134-y https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-3134-y 1475-2875 https://doaj.org/article/5c0816bd1a654b2a854d6fc3cf26cdea Malaria Journal, Vol 19, Iss 1, Pp 1-15 (2020) Plasmodium vivax Plasmodium falciparum Lactate dehydrogenase Myanmar Genetic diversity Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2020 ftdoajarticles https://doi.org/10.1186/s12936-020-3134-y 2022-12-31T10:24:37Z Abstract Background Plasmodium lactate dehydrogenase (pLDH) is a major target in diagnosing the erythrocytic stage of malaria parasites because it is highly expressed during blood-stage parasites and is distinguished from human LDH. Rapid diagnostic tests (RDTs) for malaria use pLDH as a target antigen; however, genetic variations in pLDH within the natural population threaten the efficacy of pLDH-based RDTs. Methods Genetic polymorphisms of Plasmodium vivax LDH (PvLDH) and Plasmodium falciparum LDH (PfLDH) in Myanmar isolates were analysed by nucleotide sequencing analysis. Genetic polymorphisms and the natural selection of PvLDH and PfLDH were analysed using DNASTAR, MEGA6, and DnaSP ver. 5.10.00 programs. The genetic diversity and natural selection of global PvLDH and PfLDH were also analysed. The haplotype network of global PvLDH and PfLDH was constructed using NETWORK ver. 5.0.0.3. Three-dimensional structures of PvLDH and PfLDH were built with YASARA Structure ver. 18.4.24 and the impact of mutations on structural change and stability was evaluated with SDM ver. 2, CUPSAT and MAESTROweb. Results Forty-nine PvLDH and 52 PfLDH sequences were obtained from Myanmar P. vivax and P. falciparum isolates. Non-synonymous nucleotide substitutions resulting in amino acid changes were identified in both Myanmar PvLDH and PfLDH. Amino acid changes were also identified in the global PvLDH and PfLDH populations, but they did not produce structural alterations in either protein. Low genetic diversity was observed in global PvLDH and PfLDH, which may be maintained by a strong purifying selection. Conclusion This study extends knowledge for genetic diversity and natural selection of global PvLDH and PfLDH. Although amino acid changes were observed in global PvLDH and PfLDH, they did not alter the conformational structures of the proteins. These suggest that PvLDH and PfLDH are genetically well-conserved in global populations, which indicates that they are suitable antigens for diagnostic purpose and attractive targets for ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 19 1 |
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ftdoajarticles |
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English |
topic |
Plasmodium vivax Plasmodium falciparum Lactate dehydrogenase Myanmar Genetic diversity Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Plasmodium vivax Plasmodium falciparum Lactate dehydrogenase Myanmar Genetic diversity Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Jinyoung Lee Tae Im Kim Hương Giang Lê Won Gi Yoo Jung-Mi Kang Seong-Kyu Ahn Moe Kyaw Myint Khin Lin Tong-Soo Kim Byoung-Kuk Na Genetic diversity of Plasmodium vivax and Plasmodium falciparum lactate dehydrogenases in Myanmar isolates |
topic_facet |
Plasmodium vivax Plasmodium falciparum Lactate dehydrogenase Myanmar Genetic diversity Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Plasmodium lactate dehydrogenase (pLDH) is a major target in diagnosing the erythrocytic stage of malaria parasites because it is highly expressed during blood-stage parasites and is distinguished from human LDH. Rapid diagnostic tests (RDTs) for malaria use pLDH as a target antigen; however, genetic variations in pLDH within the natural population threaten the efficacy of pLDH-based RDTs. Methods Genetic polymorphisms of Plasmodium vivax LDH (PvLDH) and Plasmodium falciparum LDH (PfLDH) in Myanmar isolates were analysed by nucleotide sequencing analysis. Genetic polymorphisms and the natural selection of PvLDH and PfLDH were analysed using DNASTAR, MEGA6, and DnaSP ver. 5.10.00 programs. The genetic diversity and natural selection of global PvLDH and PfLDH were also analysed. The haplotype network of global PvLDH and PfLDH was constructed using NETWORK ver. 5.0.0.3. Three-dimensional structures of PvLDH and PfLDH were built with YASARA Structure ver. 18.4.24 and the impact of mutations on structural change and stability was evaluated with SDM ver. 2, CUPSAT and MAESTROweb. Results Forty-nine PvLDH and 52 PfLDH sequences were obtained from Myanmar P. vivax and P. falciparum isolates. Non-synonymous nucleotide substitutions resulting in amino acid changes were identified in both Myanmar PvLDH and PfLDH. Amino acid changes were also identified in the global PvLDH and PfLDH populations, but they did not produce structural alterations in either protein. Low genetic diversity was observed in global PvLDH and PfLDH, which may be maintained by a strong purifying selection. Conclusion This study extends knowledge for genetic diversity and natural selection of global PvLDH and PfLDH. Although amino acid changes were observed in global PvLDH and PfLDH, they did not alter the conformational structures of the proteins. These suggest that PvLDH and PfLDH are genetically well-conserved in global populations, which indicates that they are suitable antigens for diagnostic purpose and attractive targets for ... |
format |
Article in Journal/Newspaper |
author |
Jinyoung Lee Tae Im Kim Hương Giang Lê Won Gi Yoo Jung-Mi Kang Seong-Kyu Ahn Moe Kyaw Myint Khin Lin Tong-Soo Kim Byoung-Kuk Na |
author_facet |
Jinyoung Lee Tae Im Kim Hương Giang Lê Won Gi Yoo Jung-Mi Kang Seong-Kyu Ahn Moe Kyaw Myint Khin Lin Tong-Soo Kim Byoung-Kuk Na |
author_sort |
Jinyoung Lee |
title |
Genetic diversity of Plasmodium vivax and Plasmodium falciparum lactate dehydrogenases in Myanmar isolates |
title_short |
Genetic diversity of Plasmodium vivax and Plasmodium falciparum lactate dehydrogenases in Myanmar isolates |
title_full |
Genetic diversity of Plasmodium vivax and Plasmodium falciparum lactate dehydrogenases in Myanmar isolates |
title_fullStr |
Genetic diversity of Plasmodium vivax and Plasmodium falciparum lactate dehydrogenases in Myanmar isolates |
title_full_unstemmed |
Genetic diversity of Plasmodium vivax and Plasmodium falciparum lactate dehydrogenases in Myanmar isolates |
title_sort |
genetic diversity of plasmodium vivax and plasmodium falciparum lactate dehydrogenases in myanmar isolates |
publisher |
BMC |
publishDate |
2020 |
url |
https://doi.org/10.1186/s12936-020-3134-y https://doaj.org/article/5c0816bd1a654b2a854d6fc3cf26cdea |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 19, Iss 1, Pp 1-15 (2020) |
op_relation |
https://doi.org/10.1186/s12936-020-3134-y https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-3134-y 1475-2875 https://doaj.org/article/5c0816bd1a654b2a854d6fc3cf26cdea |
op_doi |
https://doi.org/10.1186/s12936-020-3134-y |
container_title |
Malaria Journal |
container_volume |
19 |
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1 |
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1766345396895875072 |