Analysis of proteins from membrane and soluble fractions of Giardia duodenalis trophozoites of two Brazilian axenic strains
In the present study, we have analyzed by sodium docecyl sulphate - polyacrilamide gel electrophoresis (SDS-PAGE), immunoblotting and Concanavalin A blotting (Con A blotting) proteins of membrane fractions and soluble fractions obtained from Giardia duodenalis trophozoites of two axenic strains isol...
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Universidade de São Paulo (USP)
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ftdoajarticles:oai:doaj.org/article:5bf9044d82714cd78531b15d611af913 2024-09-09T19:25:20+00:00 Analysis of proteins from membrane and soluble fractions of Giardia duodenalis trophozoites of two Brazilian axenic strains Semíramis GUIMARÃES Maria Inês T. Leme SOGAYAR Marcello FRANCO 2002-10-01T00:00:00Z https://doaj.org/article/5bf9044d82714cd78531b15d611af913 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652002000500001&tlng=en https://doaj.org/toc/1678-9946 1678-9946 https://doaj.org/article/5bf9044d82714cd78531b15d611af913 Revista do Instituto de Medicina Tropical de São Paulo, Vol 44, Iss 5, Pp 239-244 (2002) Giardia duodenalis Axenic strains Trophozoites Proteins Glycoproteins Antigens Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2002 ftdoajarticles 2024-08-05T17:49:31Z In the present study, we have analyzed by sodium docecyl sulphate - polyacrilamide gel electrophoresis (SDS-PAGE), immunoblotting and Concanavalin A blotting (Con A blotting) proteins of membrane fractions and soluble fractions obtained from Giardia duodenalis trophozoites of two axenic strains isolated in Brazil from a symptomatic (BTU-11) and an asymptomatic patient (BTU-10), as compared to the reference strain Portland 1. Both Brazilian strains showed a complex and homogeneous electrophoretic pattern of proteins, but some differences could be observed. Several glycoproteins were detected, particularly the proteins of 81, 72, 59 kDa and the protein of 62 kDa in the membrane proteins and cytosol, respectively. Many antigenic components were revealed by anti-Giardia rabbit IgG antibodies in the immunoblotting analysis. Among these components, the membrane protein of 32 kDa and the cytosol protein of 30 kDa could be related to giardin, as previously demonstrated. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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topic |
Giardia duodenalis Axenic strains Trophozoites Proteins Glycoproteins Antigens Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Giardia duodenalis Axenic strains Trophozoites Proteins Glycoproteins Antigens Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Semíramis GUIMARÃES Maria Inês T. Leme SOGAYAR Marcello FRANCO Analysis of proteins from membrane and soluble fractions of Giardia duodenalis trophozoites of two Brazilian axenic strains |
topic_facet |
Giardia duodenalis Axenic strains Trophozoites Proteins Glycoproteins Antigens Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
In the present study, we have analyzed by sodium docecyl sulphate - polyacrilamide gel electrophoresis (SDS-PAGE), immunoblotting and Concanavalin A blotting (Con A blotting) proteins of membrane fractions and soluble fractions obtained from Giardia duodenalis trophozoites of two axenic strains isolated in Brazil from a symptomatic (BTU-11) and an asymptomatic patient (BTU-10), as compared to the reference strain Portland 1. Both Brazilian strains showed a complex and homogeneous electrophoretic pattern of proteins, but some differences could be observed. Several glycoproteins were detected, particularly the proteins of 81, 72, 59 kDa and the protein of 62 kDa in the membrane proteins and cytosol, respectively. Many antigenic components were revealed by anti-Giardia rabbit IgG antibodies in the immunoblotting analysis. Among these components, the membrane protein of 32 kDa and the cytosol protein of 30 kDa could be related to giardin, as previously demonstrated. |
format |
Article in Journal/Newspaper |
author |
Semíramis GUIMARÃES Maria Inês T. Leme SOGAYAR Marcello FRANCO |
author_facet |
Semíramis GUIMARÃES Maria Inês T. Leme SOGAYAR Marcello FRANCO |
author_sort |
Semíramis GUIMARÃES |
title |
Analysis of proteins from membrane and soluble fractions of Giardia duodenalis trophozoites of two Brazilian axenic strains |
title_short |
Analysis of proteins from membrane and soluble fractions of Giardia duodenalis trophozoites of two Brazilian axenic strains |
title_full |
Analysis of proteins from membrane and soluble fractions of Giardia duodenalis trophozoites of two Brazilian axenic strains |
title_fullStr |
Analysis of proteins from membrane and soluble fractions of Giardia duodenalis trophozoites of two Brazilian axenic strains |
title_full_unstemmed |
Analysis of proteins from membrane and soluble fractions of Giardia duodenalis trophozoites of two Brazilian axenic strains |
title_sort |
analysis of proteins from membrane and soluble fractions of giardia duodenalis trophozoites of two brazilian axenic strains |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2002 |
url |
https://doaj.org/article/5bf9044d82714cd78531b15d611af913 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 44, Iss 5, Pp 239-244 (2002) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652002000500001&tlng=en https://doaj.org/toc/1678-9946 1678-9946 https://doaj.org/article/5bf9044d82714cd78531b15d611af913 |
_version_ |
1809895068673245184 |