Presence of novel triple mutations in the pvdhfr from Plasmodium vivax in Mangaluru city area in the southwestern coastal region of India
Abstract Background Genes encoding dihydrofolate reductase (dhfr) and dihydropteroate synthase (dhps) are the targets of sulfadoxine–pyrimethamine (SP) present in artemisinin based combination therapy (ACT; artesunate + sulfadoxine pyrimethamine) for Plasmodium falciparum. Although SP is generally n...
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ftdoajarticles:oai:doaj.org/article:5bf53632201d4ef69f45f034c46a5123 2023-05-15T15:10:55+02:00 Presence of novel triple mutations in the pvdhfr from Plasmodium vivax in Mangaluru city area in the southwestern coastal region of India Shiny Joy Susanta K. Ghosh Rajeshwara N. Achur D. Channe Gowda Namita Surolia 2018-04-01T00:00:00Z https://doi.org/10.1186/s12936-018-2316-3 https://doaj.org/article/5bf53632201d4ef69f45f034c46a5123 EN eng BMC http://link.springer.com/article/10.1186/s12936-018-2316-3 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2316-3 1475-2875 https://doaj.org/article/5bf53632201d4ef69f45f034c46a5123 Malaria Journal, Vol 17, Iss 1, Pp 1-4 (2018) Dihydrofolate reductase Dihydropteroate synthase Single nucleotide polymorphisms Restriction fragment length polymorphisms Sulfadoxine/pyrimethamine Mangaluru Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2018 ftdoajarticles https://doi.org/10.1186/s12936-018-2316-3 2022-12-31T02:08:36Z Abstract Background Genes encoding dihydrofolate reductase (dhfr) and dihydropteroate synthase (dhps) are the targets of sulfadoxine–pyrimethamine (SP) present in artemisinin based combination therapy (ACT; artesunate + sulfadoxine pyrimethamine) for Plasmodium falciparum. Although SP is generally not used to treat vivax infection, mutations in dhfr and dhps that confer antifolate resistance in Plasmodium vivax are common; which may be attributed to its sympatric existence with P. falciparum. Current study was aimed to determine the pattern of mutations in dhfr and dhps in P. vivax isolates from Mangaluru region. Methods A total of 140 blood samples were collected from P. vivax-infected people attending Wenlock Hospital Mangaluru during July 2014 to January 2016. Out of 140 isolates, 25 (18%) and 50 (36%) isolates were selected randomly for sequence analysis of pvdhfr and pvdhps genes respectively. Fragment of pvdhps and full length pvdhfr were amplified, sequenced and analysed for single nucleotide polymorphisms. dhps was analysed by PCR–RFLP also, to detect the two specific mutations (A383G and A553G). Results Analysis of pvdhps sequences from 50 isolates revealed single and double mutants at 38 and 46% respectively. Three non-synonymous mutations (K55R, S58R and S117N) were identified for pvdhfr. Among these, K55R was detected for the first time. Conclusions The current study indicates that P. vivax dhps and dhfr mutant alleles are prevalent in this area, suggesting significant SP pressure. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 17 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Dihydrofolate reductase Dihydropteroate synthase Single nucleotide polymorphisms Restriction fragment length polymorphisms Sulfadoxine/pyrimethamine Mangaluru Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Dihydrofolate reductase Dihydropteroate synthase Single nucleotide polymorphisms Restriction fragment length polymorphisms Sulfadoxine/pyrimethamine Mangaluru Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Shiny Joy Susanta K. Ghosh Rajeshwara N. Achur D. Channe Gowda Namita Surolia Presence of novel triple mutations in the pvdhfr from Plasmodium vivax in Mangaluru city area in the southwestern coastal region of India |
topic_facet |
Dihydrofolate reductase Dihydropteroate synthase Single nucleotide polymorphisms Restriction fragment length polymorphisms Sulfadoxine/pyrimethamine Mangaluru Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Genes encoding dihydrofolate reductase (dhfr) and dihydropteroate synthase (dhps) are the targets of sulfadoxine–pyrimethamine (SP) present in artemisinin based combination therapy (ACT; artesunate + sulfadoxine pyrimethamine) for Plasmodium falciparum. Although SP is generally not used to treat vivax infection, mutations in dhfr and dhps that confer antifolate resistance in Plasmodium vivax are common; which may be attributed to its sympatric existence with P. falciparum. Current study was aimed to determine the pattern of mutations in dhfr and dhps in P. vivax isolates from Mangaluru region. Methods A total of 140 blood samples were collected from P. vivax-infected people attending Wenlock Hospital Mangaluru during July 2014 to January 2016. Out of 140 isolates, 25 (18%) and 50 (36%) isolates were selected randomly for sequence analysis of pvdhfr and pvdhps genes respectively. Fragment of pvdhps and full length pvdhfr were amplified, sequenced and analysed for single nucleotide polymorphisms. dhps was analysed by PCR–RFLP also, to detect the two specific mutations (A383G and A553G). Results Analysis of pvdhps sequences from 50 isolates revealed single and double mutants at 38 and 46% respectively. Three non-synonymous mutations (K55R, S58R and S117N) were identified for pvdhfr. Among these, K55R was detected for the first time. Conclusions The current study indicates that P. vivax dhps and dhfr mutant alleles are prevalent in this area, suggesting significant SP pressure. |
format |
Article in Journal/Newspaper |
author |
Shiny Joy Susanta K. Ghosh Rajeshwara N. Achur D. Channe Gowda Namita Surolia |
author_facet |
Shiny Joy Susanta K. Ghosh Rajeshwara N. Achur D. Channe Gowda Namita Surolia |
author_sort |
Shiny Joy |
title |
Presence of novel triple mutations in the pvdhfr from Plasmodium vivax in Mangaluru city area in the southwestern coastal region of India |
title_short |
Presence of novel triple mutations in the pvdhfr from Plasmodium vivax in Mangaluru city area in the southwestern coastal region of India |
title_full |
Presence of novel triple mutations in the pvdhfr from Plasmodium vivax in Mangaluru city area in the southwestern coastal region of India |
title_fullStr |
Presence of novel triple mutations in the pvdhfr from Plasmodium vivax in Mangaluru city area in the southwestern coastal region of India |
title_full_unstemmed |
Presence of novel triple mutations in the pvdhfr from Plasmodium vivax in Mangaluru city area in the southwestern coastal region of India |
title_sort |
presence of novel triple mutations in the pvdhfr from plasmodium vivax in mangaluru city area in the southwestern coastal region of india |
publisher |
BMC |
publishDate |
2018 |
url |
https://doi.org/10.1186/s12936-018-2316-3 https://doaj.org/article/5bf53632201d4ef69f45f034c46a5123 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 17, Iss 1, Pp 1-4 (2018) |
op_relation |
http://link.springer.com/article/10.1186/s12936-018-2316-3 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2316-3 1475-2875 https://doaj.org/article/5bf53632201d4ef69f45f034c46a5123 |
op_doi |
https://doi.org/10.1186/s12936-018-2316-3 |
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Malaria Journal |
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17 |
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1 |
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1766341850191364096 |