Liver gene regulation of hemostasis-related factors is altered by experimental snake envenomation in mice.
Few studies have addressed gene expression of hemostasis-related factors during acute thrombo-hemorrhagic diseases. Bites by the lanced-headed viper Bothrops jaracaca induce rapid hemostatic disturbances in victims, leading to systemic bleedings, thrombocytopenia and consumption coagulopathy. Althou...
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ftdoajarticles:oai:doaj.org/article:5bd0b5d25db84ae4826aadb1c48958c1 2023-05-15T15:13:47+02:00 Liver gene regulation of hemostasis-related factors is altered by experimental snake envenomation in mice. Ana Teresa Azevedo Sachetto José Ricardo Jensen Marcelo Larami Santoro 2020-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008379 https://doaj.org/article/5bd0b5d25db84ae4826aadb1c48958c1 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008379 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008379 https://doaj.org/article/5bd0b5d25db84ae4826aadb1c48958c1 PLoS Neglected Tropical Diseases, Vol 14, Iss 6, p e0008379 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008379 2022-12-31T05:04:46Z Few studies have addressed gene expression of hemostasis-related factors during acute thrombo-hemorrhagic diseases. Bites by the lanced-headed viper Bothrops jaracaca induce rapid hemostatic disturbances in victims, leading to systemic bleedings, thrombocytopenia and consumption coagulopathy. Although circulating levels of coagulation factors recover rapidly after administration of specific antivenom therapy, it is unclear if B. jararaca venom (BjV) upregulates the mRNA synthesis of hepatic hemostasis-related factors, or if the recovery occurs under basal conditions after the neutralization of venom components by antivenom. Thus, we aimed to investigate if BjV regulates gene expression of important hemostasis-related factors synthetized by the liver. On that account, Swiss mice were injected with saline or BjV (1.6 mg/kg b.w, s.c.), and after 3, 6 and 24 h blood samples and liver fragments were collected to analyze mRNA expression by real-time qPCR. Increased gene expression of fibrinogen chains, haptoglobin and STAT3 was observed during envenomation, particularly at 3 and 6 h. At 24h, mRNA levels of F10 were raised, while those of Serpinc1, Proc and Adamts13 were diminished. Surprisingly, F3 mRNA levels were steadily decreased at 3 h. Gene expression of Thpo, F7, F5 Tfpi, Mug1 was unaltered. mRNA levels of Vwf, P4hb, F8, F2, Plg, and Serpinf2 were minimally altered, but showed important associations with Nfkb1 gene expression. In conclusion, snakebite envenomation upregulates hepatic mRNA synthesis particularly of fibrinogen chains, and acute-phase markers. This response explains the fast recovery of fibrinogen levels after antivenom administration to patients bitten by B. jararaca snakes. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 6 e0008379 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Ana Teresa Azevedo Sachetto José Ricardo Jensen Marcelo Larami Santoro Liver gene regulation of hemostasis-related factors is altered by experimental snake envenomation in mice. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Few studies have addressed gene expression of hemostasis-related factors during acute thrombo-hemorrhagic diseases. Bites by the lanced-headed viper Bothrops jaracaca induce rapid hemostatic disturbances in victims, leading to systemic bleedings, thrombocytopenia and consumption coagulopathy. Although circulating levels of coagulation factors recover rapidly after administration of specific antivenom therapy, it is unclear if B. jararaca venom (BjV) upregulates the mRNA synthesis of hepatic hemostasis-related factors, or if the recovery occurs under basal conditions after the neutralization of venom components by antivenom. Thus, we aimed to investigate if BjV regulates gene expression of important hemostasis-related factors synthetized by the liver. On that account, Swiss mice were injected with saline or BjV (1.6 mg/kg b.w, s.c.), and after 3, 6 and 24 h blood samples and liver fragments were collected to analyze mRNA expression by real-time qPCR. Increased gene expression of fibrinogen chains, haptoglobin and STAT3 was observed during envenomation, particularly at 3 and 6 h. At 24h, mRNA levels of F10 were raised, while those of Serpinc1, Proc and Adamts13 were diminished. Surprisingly, F3 mRNA levels were steadily decreased at 3 h. Gene expression of Thpo, F7, F5 Tfpi, Mug1 was unaltered. mRNA levels of Vwf, P4hb, F8, F2, Plg, and Serpinf2 were minimally altered, but showed important associations with Nfkb1 gene expression. In conclusion, snakebite envenomation upregulates hepatic mRNA synthesis particularly of fibrinogen chains, and acute-phase markers. This response explains the fast recovery of fibrinogen levels after antivenom administration to patients bitten by B. jararaca snakes. |
format |
Article in Journal/Newspaper |
author |
Ana Teresa Azevedo Sachetto José Ricardo Jensen Marcelo Larami Santoro |
author_facet |
Ana Teresa Azevedo Sachetto José Ricardo Jensen Marcelo Larami Santoro |
author_sort |
Ana Teresa Azevedo Sachetto |
title |
Liver gene regulation of hemostasis-related factors is altered by experimental snake envenomation in mice. |
title_short |
Liver gene regulation of hemostasis-related factors is altered by experimental snake envenomation in mice. |
title_full |
Liver gene regulation of hemostasis-related factors is altered by experimental snake envenomation in mice. |
title_fullStr |
Liver gene regulation of hemostasis-related factors is altered by experimental snake envenomation in mice. |
title_full_unstemmed |
Liver gene regulation of hemostasis-related factors is altered by experimental snake envenomation in mice. |
title_sort |
liver gene regulation of hemostasis-related factors is altered by experimental snake envenomation in mice. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008379 https://doaj.org/article/5bd0b5d25db84ae4826aadb1c48958c1 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 6, p e0008379 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008379 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008379 https://doaj.org/article/5bd0b5d25db84ae4826aadb1c48958c1 |
op_doi |
https://doi.org/10.1371/journal.pntd.0008379 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
6 |
container_start_page |
e0008379 |
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1766344304218865664 |