Practical example of multiple antibody screening for evaluation of malaria control strategies
Abstract Background Ongoing efforts to fight Plasmodium falciparum malaria has reduced malaria in many areas, but new tools are needed to monitor further progress, including indicators of decreasing exposure to parasite infection. Sero-surveillance is considered promising to monitor exposure, transm...
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ftdoajarticles:oai:doaj.org/article:5b8fa6dab73d4fcdaf85882b75e946ba 2023-05-15T15:11:51+02:00 Practical example of multiple antibody screening for evaluation of malaria control strategies Marie-Louise Varela David Koffi Michael White Makhtar Niang Babacar Mbengue Fatoumata Diene Sarr André Offianan Touré Ronald Perraut 2020-03-01T00:00:00Z https://doi.org/10.1186/s12936-020-03186-9 https://doaj.org/article/5b8fa6dab73d4fcdaf85882b75e946ba EN eng BMC http://link.springer.com/article/10.1186/s12936-020-03186-9 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03186-9 1475-2875 https://doaj.org/article/5b8fa6dab73d4fcdaf85882b75e946ba Malaria Journal, Vol 19, Iss 1, Pp 1-12 (2020) Malaria Plasmodium falciparum ELISA IgG Multiple antigens Multiplex Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2020 ftdoajarticles https://doi.org/10.1186/s12936-020-03186-9 2022-12-31T00:16:15Z Abstract Background Ongoing efforts to fight Plasmodium falciparum malaria has reduced malaria in many areas, but new tools are needed to monitor further progress, including indicators of decreasing exposure to parasite infection. Sero-surveillance is considered promising to monitor exposure, transmission and immunity. Methods IgG responses to three antigen biomarkers were evaluated in a retrospective study involving: (i) surveys of 798 asymptomatic villagers from 2 Senegalese endemic settings conducted before 2002 and after the 2013 intensification of control measures, and (ii) in 105 symptomatic individuals from different settings in Côte d’Ivoire. Response to up to eight P. falciparum antigens, including recombinant MSP1p9 antigen and LSA141 peptide, were analysed using multiplex technology and responses to whole P. falciparum schizont extract (SE, local strain adapted to culture) were measured by ELISA. Results MSP1p9 and LSA141 IgG responses were shown to be relevant indicators monitoring immune status in the different study sites both from Côte d’Ivoire and Senegal. Between 2002 and 2013, individuals participating in both studies showed higher decline of sero-positivity in young (< 15 years: range 12% to 50%) than older (> 15 years: no decline to 15%) individuals from Dielmo and Ndiop. A mathematical sero-catalytic model from the complete Dielmo/Ndiop survey was used to reconstruct declining levels of sero-positivity in more detail, demonstrating that anti-SE seroprevalence levels most accurately reflected malaria exposure in the two villages. Conclusion For standard screening of population immune status at sites envisaging elimination, the use of ELISA-based assays targeting selected antigens can contribute to provide important epidemiologic surveillance data to aid malaria control programmes. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 19 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Malaria Plasmodium falciparum ELISA IgG Multiple antigens Multiplex Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Malaria Plasmodium falciparum ELISA IgG Multiple antigens Multiplex Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Marie-Louise Varela David Koffi Michael White Makhtar Niang Babacar Mbengue Fatoumata Diene Sarr André Offianan Touré Ronald Perraut Practical example of multiple antibody screening for evaluation of malaria control strategies |
topic_facet |
Malaria Plasmodium falciparum ELISA IgG Multiple antigens Multiplex Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Ongoing efforts to fight Plasmodium falciparum malaria has reduced malaria in many areas, but new tools are needed to monitor further progress, including indicators of decreasing exposure to parasite infection. Sero-surveillance is considered promising to monitor exposure, transmission and immunity. Methods IgG responses to three antigen biomarkers were evaluated in a retrospective study involving: (i) surveys of 798 asymptomatic villagers from 2 Senegalese endemic settings conducted before 2002 and after the 2013 intensification of control measures, and (ii) in 105 symptomatic individuals from different settings in Côte d’Ivoire. Response to up to eight P. falciparum antigens, including recombinant MSP1p9 antigen and LSA141 peptide, were analysed using multiplex technology and responses to whole P. falciparum schizont extract (SE, local strain adapted to culture) were measured by ELISA. Results MSP1p9 and LSA141 IgG responses were shown to be relevant indicators monitoring immune status in the different study sites both from Côte d’Ivoire and Senegal. Between 2002 and 2013, individuals participating in both studies showed higher decline of sero-positivity in young (< 15 years: range 12% to 50%) than older (> 15 years: no decline to 15%) individuals from Dielmo and Ndiop. A mathematical sero-catalytic model from the complete Dielmo/Ndiop survey was used to reconstruct declining levels of sero-positivity in more detail, demonstrating that anti-SE seroprevalence levels most accurately reflected malaria exposure in the two villages. Conclusion For standard screening of population immune status at sites envisaging elimination, the use of ELISA-based assays targeting selected antigens can contribute to provide important epidemiologic surveillance data to aid malaria control programmes. |
format |
Article in Journal/Newspaper |
author |
Marie-Louise Varela David Koffi Michael White Makhtar Niang Babacar Mbengue Fatoumata Diene Sarr André Offianan Touré Ronald Perraut |
author_facet |
Marie-Louise Varela David Koffi Michael White Makhtar Niang Babacar Mbengue Fatoumata Diene Sarr André Offianan Touré Ronald Perraut |
author_sort |
Marie-Louise Varela |
title |
Practical example of multiple antibody screening for evaluation of malaria control strategies |
title_short |
Practical example of multiple antibody screening for evaluation of malaria control strategies |
title_full |
Practical example of multiple antibody screening for evaluation of malaria control strategies |
title_fullStr |
Practical example of multiple antibody screening for evaluation of malaria control strategies |
title_full_unstemmed |
Practical example of multiple antibody screening for evaluation of malaria control strategies |
title_sort |
practical example of multiple antibody screening for evaluation of malaria control strategies |
publisher |
BMC |
publishDate |
2020 |
url |
https://doi.org/10.1186/s12936-020-03186-9 https://doaj.org/article/5b8fa6dab73d4fcdaf85882b75e946ba |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 19, Iss 1, Pp 1-12 (2020) |
op_relation |
http://link.springer.com/article/10.1186/s12936-020-03186-9 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03186-9 1475-2875 https://doaj.org/article/5b8fa6dab73d4fcdaf85882b75e946ba |
op_doi |
https://doi.org/10.1186/s12936-020-03186-9 |
container_title |
Malaria Journal |
container_volume |
19 |
container_issue |
1 |
_version_ |
1766342627964223488 |