Colonization factors among enterotoxigenic Escherichia coli isolates from children with moderate-to-severe diarrhea and from matched controls in the Global Enteric Multicenter Study (GEMS).
Background Enterotoxigenic Escherichia coli (ETEC) encoding heat-stable enterotoxin (ST) alone or with heat-labile enterotoxin (LT) cause moderate-to-severe diarrhea (MSD) in developing country children. The Global Enteric Multicenter Study (GEMS) identified ETEC encoding ST among the top four enter...
Published in: | PLOS Neglected Tropical Diseases |
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Online Access: | https://doi.org/10.1371/journal.pntd.0007037 https://doaj.org/article/5b8457f7fd464a51bfcffb0d38a82a9e |
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ftdoajarticles:oai:doaj.org/article:5b8457f7fd464a51bfcffb0d38a82a9e 2023-05-15T15:17:46+02:00 Colonization factors among enterotoxigenic Escherichia coli isolates from children with moderate-to-severe diarrhea and from matched controls in the Global Enteric Multicenter Study (GEMS). Roberto M Vidal Khitam Muhsen Sharon M Tennant Ann-Mari Svennerholm Samba O Sow Dipika Sur Anita K M Zaidi Abu S G Faruque Debasish Saha Richard Adegbola M Jahangir Hossain Pedro L Alonso Robert F Breiman Quique Bassat Boubou Tamboura Doh Sanogo Uma Onwuchekwa Byomkesh Manna Thandavarayan Ramamurthy Suman Kanungo Shahnawaz Ahmed Shahida Qureshi Farheen Quadri Anowar Hossain Sumon K Das Martin Antonio Inacio Mandomando Tacilta Nhampossa Sozinho Acácio Richard Omore John B Ochieng Joseph O Oundo Eric D Mintz Ciara E O'Reilly Lynette Y Berkeley Sofie Livio Sandra Panchalingam Dilruba Nasrin Tamer H Farag Yukun Wu Halvor Sommerfelt Roy M Robins-Browne Felipe Del Canto Tracy H Hazen David A Rasko Karen L Kotloff James P Nataro Myron M Levine 2019-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007037 https://doaj.org/article/5b8457f7fd464a51bfcffb0d38a82a9e EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007037 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007037 https://doaj.org/article/5b8457f7fd464a51bfcffb0d38a82a9e PLoS Neglected Tropical Diseases, Vol 13, Iss 1, p e0007037 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007037 2022-12-31T05:59:59Z Background Enterotoxigenic Escherichia coli (ETEC) encoding heat-stable enterotoxin (ST) alone or with heat-labile enterotoxin (LT) cause moderate-to-severe diarrhea (MSD) in developing country children. The Global Enteric Multicenter Study (GEMS) identified ETEC encoding ST among the top four enteropathogens. Since the GEMS objective was to provide evidence to guide development and implementation of enteric vaccines and other interventions to diminish diarrheal disease morbidity and mortality, we examined colonization factor (CF) prevalence among ETEC isolates from children age <5 years with MSD and from matched controls in four African and three Asian sites. We also assessed strength of association of specific CFs with MSD. Methodology/principal findings MSD cases enrolled at healthcare facilities over three years and matched controls were tested in a standardized manner for many enteropathogens. To identify ETEC, three E. coli colonies per child were tested by polymerase chain reaction (PCR) to detect genes encoding LT, ST; confirmed ETEC were examined by PCR for major CFs (Colonization Factor Antigen I [CFA/I] or Coli Surface [CS] antigens CS1-CS6) and minor CFs (CS7, CS12, CS13, CS14, CS17, CS18, CS19, CS20, CS21, CS30). ETEC from 806 cases had a single toxin/CF profile in three tested strains per child. Major CFs, components of multiple ETEC vaccine candidates, were detected in 66.0% of LT/ST and ST-only cases and were associated with MSD versus matched controls by conditional logistic regression (p≤0.006); major CFs detected in only 25.0% of LT-only cases weren't associated with MSD. ETEC encoding exclusively CS14, identified among 19.9% of 291 ST-only and 1.5% of 259 LT/ST strains, were associated with MSD (p = 0.0011). No other minor CF exhibited prevalence ≥5% and significant association with MSD. Conclusions/significance Major CF-based efficacious ETEC vaccines could potentially prevent up to 66% of pediatric MSD cases due to ST-encoding ETEC in developing countries; adding CS14 extends coverage to ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 1 e0007037 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Roberto M Vidal Khitam Muhsen Sharon M Tennant Ann-Mari Svennerholm Samba O Sow Dipika Sur Anita K M Zaidi Abu S G Faruque Debasish Saha Richard Adegbola M Jahangir Hossain Pedro L Alonso Robert F Breiman Quique Bassat Boubou Tamboura Doh Sanogo Uma Onwuchekwa Byomkesh Manna Thandavarayan Ramamurthy Suman Kanungo Shahnawaz Ahmed Shahida Qureshi Farheen Quadri Anowar Hossain Sumon K Das Martin Antonio Inacio Mandomando Tacilta Nhampossa Sozinho Acácio Richard Omore John B Ochieng Joseph O Oundo Eric D Mintz Ciara E O'Reilly Lynette Y Berkeley Sofie Livio Sandra Panchalingam Dilruba Nasrin Tamer H Farag Yukun Wu Halvor Sommerfelt Roy M Robins-Browne Felipe Del Canto Tracy H Hazen David A Rasko Karen L Kotloff James P Nataro Myron M Levine Colonization factors among enterotoxigenic Escherichia coli isolates from children with moderate-to-severe diarrhea and from matched controls in the Global Enteric Multicenter Study (GEMS). |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Enterotoxigenic Escherichia coli (ETEC) encoding heat-stable enterotoxin (ST) alone or with heat-labile enterotoxin (LT) cause moderate-to-severe diarrhea (MSD) in developing country children. The Global Enteric Multicenter Study (GEMS) identified ETEC encoding ST among the top four enteropathogens. Since the GEMS objective was to provide evidence to guide development and implementation of enteric vaccines and other interventions to diminish diarrheal disease morbidity and mortality, we examined colonization factor (CF) prevalence among ETEC isolates from children age <5 years with MSD and from matched controls in four African and three Asian sites. We also assessed strength of association of specific CFs with MSD. Methodology/principal findings MSD cases enrolled at healthcare facilities over three years and matched controls were tested in a standardized manner for many enteropathogens. To identify ETEC, three E. coli colonies per child were tested by polymerase chain reaction (PCR) to detect genes encoding LT, ST; confirmed ETEC were examined by PCR for major CFs (Colonization Factor Antigen I [CFA/I] or Coli Surface [CS] antigens CS1-CS6) and minor CFs (CS7, CS12, CS13, CS14, CS17, CS18, CS19, CS20, CS21, CS30). ETEC from 806 cases had a single toxin/CF profile in three tested strains per child. Major CFs, components of multiple ETEC vaccine candidates, were detected in 66.0% of LT/ST and ST-only cases and were associated with MSD versus matched controls by conditional logistic regression (p≤0.006); major CFs detected in only 25.0% of LT-only cases weren't associated with MSD. ETEC encoding exclusively CS14, identified among 19.9% of 291 ST-only and 1.5% of 259 LT/ST strains, were associated with MSD (p = 0.0011). No other minor CF exhibited prevalence ≥5% and significant association with MSD. Conclusions/significance Major CF-based efficacious ETEC vaccines could potentially prevent up to 66% of pediatric MSD cases due to ST-encoding ETEC in developing countries; adding CS14 extends coverage to ... |
format |
Article in Journal/Newspaper |
author |
Roberto M Vidal Khitam Muhsen Sharon M Tennant Ann-Mari Svennerholm Samba O Sow Dipika Sur Anita K M Zaidi Abu S G Faruque Debasish Saha Richard Adegbola M Jahangir Hossain Pedro L Alonso Robert F Breiman Quique Bassat Boubou Tamboura Doh Sanogo Uma Onwuchekwa Byomkesh Manna Thandavarayan Ramamurthy Suman Kanungo Shahnawaz Ahmed Shahida Qureshi Farheen Quadri Anowar Hossain Sumon K Das Martin Antonio Inacio Mandomando Tacilta Nhampossa Sozinho Acácio Richard Omore John B Ochieng Joseph O Oundo Eric D Mintz Ciara E O'Reilly Lynette Y Berkeley Sofie Livio Sandra Panchalingam Dilruba Nasrin Tamer H Farag Yukun Wu Halvor Sommerfelt Roy M Robins-Browne Felipe Del Canto Tracy H Hazen David A Rasko Karen L Kotloff James P Nataro Myron M Levine |
author_facet |
Roberto M Vidal Khitam Muhsen Sharon M Tennant Ann-Mari Svennerholm Samba O Sow Dipika Sur Anita K M Zaidi Abu S G Faruque Debasish Saha Richard Adegbola M Jahangir Hossain Pedro L Alonso Robert F Breiman Quique Bassat Boubou Tamboura Doh Sanogo Uma Onwuchekwa Byomkesh Manna Thandavarayan Ramamurthy Suman Kanungo Shahnawaz Ahmed Shahida Qureshi Farheen Quadri Anowar Hossain Sumon K Das Martin Antonio Inacio Mandomando Tacilta Nhampossa Sozinho Acácio Richard Omore John B Ochieng Joseph O Oundo Eric D Mintz Ciara E O'Reilly Lynette Y Berkeley Sofie Livio Sandra Panchalingam Dilruba Nasrin Tamer H Farag Yukun Wu Halvor Sommerfelt Roy M Robins-Browne Felipe Del Canto Tracy H Hazen David A Rasko Karen L Kotloff James P Nataro Myron M Levine |
author_sort |
Roberto M Vidal |
title |
Colonization factors among enterotoxigenic Escherichia coli isolates from children with moderate-to-severe diarrhea and from matched controls in the Global Enteric Multicenter Study (GEMS). |
title_short |
Colonization factors among enterotoxigenic Escherichia coli isolates from children with moderate-to-severe diarrhea and from matched controls in the Global Enteric Multicenter Study (GEMS). |
title_full |
Colonization factors among enterotoxigenic Escherichia coli isolates from children with moderate-to-severe diarrhea and from matched controls in the Global Enteric Multicenter Study (GEMS). |
title_fullStr |
Colonization factors among enterotoxigenic Escherichia coli isolates from children with moderate-to-severe diarrhea and from matched controls in the Global Enteric Multicenter Study (GEMS). |
title_full_unstemmed |
Colonization factors among enterotoxigenic Escherichia coli isolates from children with moderate-to-severe diarrhea and from matched controls in the Global Enteric Multicenter Study (GEMS). |
title_sort |
colonization factors among enterotoxigenic escherichia coli isolates from children with moderate-to-severe diarrhea and from matched controls in the global enteric multicenter study (gems). |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007037 https://doaj.org/article/5b8457f7fd464a51bfcffb0d38a82a9e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 1, p e0007037 (2019) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007037 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007037 https://doaj.org/article/5b8457f7fd464a51bfcffb0d38a82a9e |
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https://doi.org/10.1371/journal.pntd.0007037 |
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PLOS Neglected Tropical Diseases |
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