Role of invasive carnivores (Procyon lotor and Nyctereutes procyonoides) in epidemiology of vector-borne pathogens: molecular survey from the Czech Republic
Abstract Background Vector-borne pathogens (VBPs) are a major threat to humans, livestock and companion animals worldwide. The combined effect of climatic, socioeconomic and host composition changes favours the spread of the vectors, together with the expansion of invasive carnivores contributing to...
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ftdoajarticles:oai:doaj.org/article:5b3f14de6672404c910abe4f7d6011a1 2023-07-30T04:02:52+02:00 Role of invasive carnivores (Procyon lotor and Nyctereutes procyonoides) in epidemiology of vector-borne pathogens: molecular survey from the Czech Republic Ondřej Daněk Paulina Maria Lesiczka Iva Hammerbauerova Karolina Volfova Jana Juránková Lucia Frgelecová David Modrý Kristyna Hrazdilova 2023-07-01T00:00:00Z https://doi.org/10.1186/s13071-023-05834-w https://doaj.org/article/5b3f14de6672404c910abe4f7d6011a1 EN eng BMC https://doi.org/10.1186/s13071-023-05834-w https://doaj.org/toc/1756-3305 doi:10.1186/s13071-023-05834-w 1756-3305 https://doaj.org/article/5b3f14de6672404c910abe4f7d6011a1 Parasites & Vectors, Vol 16, Iss 1, Pp 1-14 (2023) Carnivores Invasive species Vector-borne pathogens Anaplasma phagocytophilum Hepatozoon Babesia Infectious and parasitic diseases RC109-216 article 2023 ftdoajarticles https://doi.org/10.1186/s13071-023-05834-w 2023-07-16T00:38:41Z Abstract Background Vector-borne pathogens (VBPs) are a major threat to humans, livestock and companion animals worldwide. The combined effect of climatic, socioeconomic and host composition changes favours the spread of the vectors, together with the expansion of invasive carnivores contributing to the spread of the pathogens. In Europe, the most widespread invasive species of carnivores are raccoons (Procyon lotor) and raccoon dogs (Nyctereutes procyonoides). This study focused on the detection of four major groups of VBPs namely Babesia, Hepatozoon, Anaplasma phagocytophilum and Bartonella in invasive and native carnivores in the Czech Republic, with the emphasis on the role of invasive carnivores in the eco-epidemiology of said VBPs. Methods Spleen samples of 84 carnivores of eight species (Canis aureus, Canis lupus, Lynx lynx, P. lotor, Martes foina, Lutra lutra, Mustela erminea and N. procyonoides) were screened by combined nested PCR and sequencing for the above-mentioned VBPs targeting 18S rRNA and cytB in hemoprotozoa, groEL in A. phagocytophilum, and using multilocus genotyping in Bartonella spp. The species determination is supported by phylogenetic analysis inferred by the maximum likelihood method. Results Out of 84 samples, 44% tested positive for at least one pathogen. Five different species of VBPs were detected in P. lotor, namely Bartonella canis, Hepatozoon canis, Hepatozoon martis, A. phagocytophilum and Bartonella sp. related to Bartonella washoensis. All C. lupus tested positive for H. canis and one for B. canis. Three VBPs (Hepatozoon silvestris, A. phagocytophilum and Bartonella taylorii) were detected in L. lynx for the first time. Babesia vulpes and yet undescribed species of Babesia, not previously detected in Europe, were found in N. procyonoides. Conclusions Wild carnivores in the Czech Republic are hosts of several VBPs with potential veterinary and public health risks. Among the studied carnivore species, the invasive raccoon is the most competent host. Raccoons are the only ... Article in Journal/Newspaper Canis lupus Lutra lutra Lynx Directory of Open Access Journals: DOAJ Articles Parasites & Vectors 16 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Carnivores Invasive species Vector-borne pathogens Anaplasma phagocytophilum Hepatozoon Babesia Infectious and parasitic diseases RC109-216 |
spellingShingle |
Carnivores Invasive species Vector-borne pathogens Anaplasma phagocytophilum Hepatozoon Babesia Infectious and parasitic diseases RC109-216 Ondřej Daněk Paulina Maria Lesiczka Iva Hammerbauerova Karolina Volfova Jana Juránková Lucia Frgelecová David Modrý Kristyna Hrazdilova Role of invasive carnivores (Procyon lotor and Nyctereutes procyonoides) in epidemiology of vector-borne pathogens: molecular survey from the Czech Republic |
topic_facet |
Carnivores Invasive species Vector-borne pathogens Anaplasma phagocytophilum Hepatozoon Babesia Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Vector-borne pathogens (VBPs) are a major threat to humans, livestock and companion animals worldwide. The combined effect of climatic, socioeconomic and host composition changes favours the spread of the vectors, together with the expansion of invasive carnivores contributing to the spread of the pathogens. In Europe, the most widespread invasive species of carnivores are raccoons (Procyon lotor) and raccoon dogs (Nyctereutes procyonoides). This study focused on the detection of four major groups of VBPs namely Babesia, Hepatozoon, Anaplasma phagocytophilum and Bartonella in invasive and native carnivores in the Czech Republic, with the emphasis on the role of invasive carnivores in the eco-epidemiology of said VBPs. Methods Spleen samples of 84 carnivores of eight species (Canis aureus, Canis lupus, Lynx lynx, P. lotor, Martes foina, Lutra lutra, Mustela erminea and N. procyonoides) were screened by combined nested PCR and sequencing for the above-mentioned VBPs targeting 18S rRNA and cytB in hemoprotozoa, groEL in A. phagocytophilum, and using multilocus genotyping in Bartonella spp. The species determination is supported by phylogenetic analysis inferred by the maximum likelihood method. Results Out of 84 samples, 44% tested positive for at least one pathogen. Five different species of VBPs were detected in P. lotor, namely Bartonella canis, Hepatozoon canis, Hepatozoon martis, A. phagocytophilum and Bartonella sp. related to Bartonella washoensis. All C. lupus tested positive for H. canis and one for B. canis. Three VBPs (Hepatozoon silvestris, A. phagocytophilum and Bartonella taylorii) were detected in L. lynx for the first time. Babesia vulpes and yet undescribed species of Babesia, not previously detected in Europe, were found in N. procyonoides. Conclusions Wild carnivores in the Czech Republic are hosts of several VBPs with potential veterinary and public health risks. Among the studied carnivore species, the invasive raccoon is the most competent host. Raccoons are the only ... |
format |
Article in Journal/Newspaper |
author |
Ondřej Daněk Paulina Maria Lesiczka Iva Hammerbauerova Karolina Volfova Jana Juránková Lucia Frgelecová David Modrý Kristyna Hrazdilova |
author_facet |
Ondřej Daněk Paulina Maria Lesiczka Iva Hammerbauerova Karolina Volfova Jana Juránková Lucia Frgelecová David Modrý Kristyna Hrazdilova |
author_sort |
Ondřej Daněk |
title |
Role of invasive carnivores (Procyon lotor and Nyctereutes procyonoides) in epidemiology of vector-borne pathogens: molecular survey from the Czech Republic |
title_short |
Role of invasive carnivores (Procyon lotor and Nyctereutes procyonoides) in epidemiology of vector-borne pathogens: molecular survey from the Czech Republic |
title_full |
Role of invasive carnivores (Procyon lotor and Nyctereutes procyonoides) in epidemiology of vector-borne pathogens: molecular survey from the Czech Republic |
title_fullStr |
Role of invasive carnivores (Procyon lotor and Nyctereutes procyonoides) in epidemiology of vector-borne pathogens: molecular survey from the Czech Republic |
title_full_unstemmed |
Role of invasive carnivores (Procyon lotor and Nyctereutes procyonoides) in epidemiology of vector-borne pathogens: molecular survey from the Czech Republic |
title_sort |
role of invasive carnivores (procyon lotor and nyctereutes procyonoides) in epidemiology of vector-borne pathogens: molecular survey from the czech republic |
publisher |
BMC |
publishDate |
2023 |
url |
https://doi.org/10.1186/s13071-023-05834-w https://doaj.org/article/5b3f14de6672404c910abe4f7d6011a1 |
genre |
Canis lupus Lutra lutra Lynx |
genre_facet |
Canis lupus Lutra lutra Lynx |
op_source |
Parasites & Vectors, Vol 16, Iss 1, Pp 1-14 (2023) |
op_relation |
https://doi.org/10.1186/s13071-023-05834-w https://doaj.org/toc/1756-3305 doi:10.1186/s13071-023-05834-w 1756-3305 https://doaj.org/article/5b3f14de6672404c910abe4f7d6011a1 |
op_doi |
https://doi.org/10.1186/s13071-023-05834-w |
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Parasites & Vectors |
container_volume |
16 |
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