Demethylbelamcandaquinone B from Marantodes pumilum var. alata (Blume) Kuntze inhibits osteoclast differentiation in RAW264.7 cells
Objective: To investigate the bone-resorbing effect of demethylbelamcandaquinone B (Dmcq B) extracted from Marantodes pumilum var. alata on osteoclast differentiation in RAW264.7 cells. Methods: RAW264.7 macrophages were differentiated using RANKL into osteoclast-like cells. Then, they were treated...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2021
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Online Access: | https://doi.org/10.4103/2221-1691.331269 https://doaj.org/article/5b1d9bcd57fc41b896fbf3d2267cf824 |
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ftdoajarticles:oai:doaj.org/article:5b1d9bcd57fc41b896fbf3d2267cf824 2023-05-15T15:09:16+02:00 Demethylbelamcandaquinone B from Marantodes pumilum var. alata (Blume) Kuntze inhibits osteoclast differentiation in RAW264.7 cells Haryati Ahmad Hairi Jamia Azdina Jamal Nor Ashila Aladdin Khairana Husain Noor Suhaili Mohd Sofi Norazlina Mohamed Isa Naina Mohamed Ahmad Nazrun Shuid 2021-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.331269 https://doaj.org/article/5b1d9bcd57fc41b896fbf3d2267cf824 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=12;spage=535;epage=542;aulast=Ahmad https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.331269 https://doaj.org/article/5b1d9bcd57fc41b896fbf3d2267cf824 Asian Pacific Journal of Tropical Biomedicine, Vol 11, Iss 12, Pp 535-542 (2021) marantodes pumilum var. alata phytoestrogen raw264.7 induced-osteoclast bone-resorbing effect Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2021 ftdoajarticles https://doi.org/10.4103/2221-1691.331269 2022-12-30T19:54:40Z Objective: To investigate the bone-resorbing effect of demethylbelamcandaquinone B (Dmcq B) extracted from Marantodes pumilum var. alata on osteoclast differentiation in RAW264.7 cells. Methods: RAW264.7 macrophages were differentiated using RANKL into osteoclast-like cells. Then, they were treated with 10 μg/mL Marantodes pumilum var. alata crude aqueous extract, 5 μg/ mL dichloromethane fraction, and 0.6 μg/mL Dmcq B and 0.06 μg/ mL estradiol. Tartrate-resistant acid phosphatase 5b (TRACP 5b) as an osteoclast phenotypic marker was determined by TRACP staining and TRACP 5b colometric assay, and bone-resorbing pits were examined. The gene expression of pro-inflammatory cytokines (TNF-α and IL-6) was measured. Moreover, the protein expressions of pro-inflammatory cytokines (TNF-α and IL-6) and estrogen receptors were evaluated. Results: Marantodes pumilum var. alata crude aqueous extract and Dmcq B inhibited RANKL-stimulated osteoclast differentiation as evidenced by size reduction of giant multinucleated osteoclast cells, decreased TRACP 5b activity as well as the subsiding of resorbed pit area compared with normal control. In addition, they reduced the gene and protein expressions of TNF-α and IL-6. Marantodes pumilum var. alata, Dmcq B, and estradiol treatments increased the protein expressions of estrogen receptors alpha and beta in osteoclasts. Conclusions: Marantodes pumilum var. alata and its active compound, Dmcq B can inhibit osteoclast differentiation. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 11 12 535 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
marantodes pumilum var. alata phytoestrogen raw264.7 induced-osteoclast bone-resorbing effect Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
marantodes pumilum var. alata phytoestrogen raw264.7 induced-osteoclast bone-resorbing effect Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Haryati Ahmad Hairi Jamia Azdina Jamal Nor Ashila Aladdin Khairana Husain Noor Suhaili Mohd Sofi Norazlina Mohamed Isa Naina Mohamed Ahmad Nazrun Shuid Demethylbelamcandaquinone B from Marantodes pumilum var. alata (Blume) Kuntze inhibits osteoclast differentiation in RAW264.7 cells |
topic_facet |
marantodes pumilum var. alata phytoestrogen raw264.7 induced-osteoclast bone-resorbing effect Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate the bone-resorbing effect of demethylbelamcandaquinone B (Dmcq B) extracted from Marantodes pumilum var. alata on osteoclast differentiation in RAW264.7 cells. Methods: RAW264.7 macrophages were differentiated using RANKL into osteoclast-like cells. Then, they were treated with 10 μg/mL Marantodes pumilum var. alata crude aqueous extract, 5 μg/ mL dichloromethane fraction, and 0.6 μg/mL Dmcq B and 0.06 μg/ mL estradiol. Tartrate-resistant acid phosphatase 5b (TRACP 5b) as an osteoclast phenotypic marker was determined by TRACP staining and TRACP 5b colometric assay, and bone-resorbing pits were examined. The gene expression of pro-inflammatory cytokines (TNF-α and IL-6) was measured. Moreover, the protein expressions of pro-inflammatory cytokines (TNF-α and IL-6) and estrogen receptors were evaluated. Results: Marantodes pumilum var. alata crude aqueous extract and Dmcq B inhibited RANKL-stimulated osteoclast differentiation as evidenced by size reduction of giant multinucleated osteoclast cells, decreased TRACP 5b activity as well as the subsiding of resorbed pit area compared with normal control. In addition, they reduced the gene and protein expressions of TNF-α and IL-6. Marantodes pumilum var. alata, Dmcq B, and estradiol treatments increased the protein expressions of estrogen receptors alpha and beta in osteoclasts. Conclusions: Marantodes pumilum var. alata and its active compound, Dmcq B can inhibit osteoclast differentiation. |
format |
Article in Journal/Newspaper |
author |
Haryati Ahmad Hairi Jamia Azdina Jamal Nor Ashila Aladdin Khairana Husain Noor Suhaili Mohd Sofi Norazlina Mohamed Isa Naina Mohamed Ahmad Nazrun Shuid |
author_facet |
Haryati Ahmad Hairi Jamia Azdina Jamal Nor Ashila Aladdin Khairana Husain Noor Suhaili Mohd Sofi Norazlina Mohamed Isa Naina Mohamed Ahmad Nazrun Shuid |
author_sort |
Haryati Ahmad Hairi |
title |
Demethylbelamcandaquinone B from Marantodes pumilum var. alata (Blume) Kuntze inhibits osteoclast differentiation in RAW264.7 cells |
title_short |
Demethylbelamcandaquinone B from Marantodes pumilum var. alata (Blume) Kuntze inhibits osteoclast differentiation in RAW264.7 cells |
title_full |
Demethylbelamcandaquinone B from Marantodes pumilum var. alata (Blume) Kuntze inhibits osteoclast differentiation in RAW264.7 cells |
title_fullStr |
Demethylbelamcandaquinone B from Marantodes pumilum var. alata (Blume) Kuntze inhibits osteoclast differentiation in RAW264.7 cells |
title_full_unstemmed |
Demethylbelamcandaquinone B from Marantodes pumilum var. alata (Blume) Kuntze inhibits osteoclast differentiation in RAW264.7 cells |
title_sort |
demethylbelamcandaquinone b from marantodes pumilum var. alata (blume) kuntze inhibits osteoclast differentiation in raw264.7 cells |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2021 |
url |
https://doi.org/10.4103/2221-1691.331269 https://doaj.org/article/5b1d9bcd57fc41b896fbf3d2267cf824 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 11, Iss 12, Pp 535-542 (2021) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=12;spage=535;epage=542;aulast=Ahmad https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.331269 https://doaj.org/article/5b1d9bcd57fc41b896fbf3d2267cf824 |
op_doi |
https://doi.org/10.4103/2221-1691.331269 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
11 |
container_issue |
12 |
container_start_page |
535 |
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1766340486926172160 |