A C-type lectin from Bothrops jararacussu venom can adhere to extracellular matrix proteins and induce the rolling of leukocytes
Purification of a lectin from Bothrops jararacussu venom (BjcuL) was carried out using agarose-D-galactose affinity gel. MALDI-TOF gave a major signal at m/z 32028, suggesting the presence of a dimmer composed of two identical subunits. Divalent cations were required for the lectin activity, as comp...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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Online Access: | https://doi.org/10.1590/S1678-91992007000400009 https://doaj.org/article/5aeed971530247a2b933c4b3f9fab6e8 |
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ftdoajarticles:oai:doaj.org/article:5aeed971530247a2b933c4b3f9fab6e8 2023-05-15T15:10:25+02:00 A C-type lectin from Bothrops jararacussu venom can adhere to extracellular matrix proteins and induce the rolling of leukocytes S. L. Elífio-Esposito P. L. Hess A. N. Moreno M. Lopes-Ferreira C. A. O. Ricart M. V. Souza F. Hasselman-Zielinski J. A. Becker L. F. Pereira 2007-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992007000400009 https://doaj.org/article/5aeed971530247a2b933c4b3f9fab6e8 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400009 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992007000400009 1678-9199 https://doaj.org/article/5aeed971530247a2b933c4b3f9fab6e8 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 13, Iss 4, Pp 782-799 (2007) venoms fibronectins vitronectin snakes intravital microscopy leukocytes Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2007 ftdoajarticles https://doi.org/10.1590/S1678-91992007000400009 2022-12-31T14:11:48Z Purification of a lectin from Bothrops jararacussu venom (BjcuL) was carried out using agarose-D-galactose affinity gel. MALDI-TOF gave a major signal at m/z 32028, suggesting the presence of a dimmer composed of two identical subunits. Divalent cations were required for the lectin activity, as complete absence of such ions reduced hemagglutination. BjcuL was more effective at neutral pH and showed total loss of activity at pH values below 4.0 and above 9.0. Its agglutinating activity remained stable at 25°C until 60min, but increased when at 35°C for at least 15min. Adhesion assays to extracellular matrix (ECM) glycoproteins showed that the biotinylated lectin (0.039-5.0µg/100µl) was capable of binding to fibronectin and vitronectin in a dose-dependent manner. The binding was partially inhibited in the presence of D-galactose. BjcuL (1.25-10µg/30µl) potential was investigated for leukocyte rolling and adhesion to endothelial cells in living microvessels using intravital microscopy, which showed that it induced a dose-dependent increase in rolling and adherence of leukocytes, acting directly on endothelial cells of postcapillary venules. The specific association between lectins and their ligands, either on the cell surface or on the ECM, is related to a variety of biological processes. The complementary characterization of BjcuL, shown here, is useful to further understand the venom effects and as a background for future investigation for therapeutic strategies. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 13 4 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
venoms fibronectins vitronectin snakes intravital microscopy leukocytes Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
venoms fibronectins vitronectin snakes intravital microscopy leukocytes Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 S. L. Elífio-Esposito P. L. Hess A. N. Moreno M. Lopes-Ferreira C. A. O. Ricart M. V. Souza F. Hasselman-Zielinski J. A. Becker L. F. Pereira A C-type lectin from Bothrops jararacussu venom can adhere to extracellular matrix proteins and induce the rolling of leukocytes |
topic_facet |
venoms fibronectins vitronectin snakes intravital microscopy leukocytes Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Purification of a lectin from Bothrops jararacussu venom (BjcuL) was carried out using agarose-D-galactose affinity gel. MALDI-TOF gave a major signal at m/z 32028, suggesting the presence of a dimmer composed of two identical subunits. Divalent cations were required for the lectin activity, as complete absence of such ions reduced hemagglutination. BjcuL was more effective at neutral pH and showed total loss of activity at pH values below 4.0 and above 9.0. Its agglutinating activity remained stable at 25°C until 60min, but increased when at 35°C for at least 15min. Adhesion assays to extracellular matrix (ECM) glycoproteins showed that the biotinylated lectin (0.039-5.0µg/100µl) was capable of binding to fibronectin and vitronectin in a dose-dependent manner. The binding was partially inhibited in the presence of D-galactose. BjcuL (1.25-10µg/30µl) potential was investigated for leukocyte rolling and adhesion to endothelial cells in living microvessels using intravital microscopy, which showed that it induced a dose-dependent increase in rolling and adherence of leukocytes, acting directly on endothelial cells of postcapillary venules. The specific association between lectins and their ligands, either on the cell surface or on the ECM, is related to a variety of biological processes. The complementary characterization of BjcuL, shown here, is useful to further understand the venom effects and as a background for future investigation for therapeutic strategies. |
format |
Article in Journal/Newspaper |
author |
S. L. Elífio-Esposito P. L. Hess A. N. Moreno M. Lopes-Ferreira C. A. O. Ricart M. V. Souza F. Hasselman-Zielinski J. A. Becker L. F. Pereira |
author_facet |
S. L. Elífio-Esposito P. L. Hess A. N. Moreno M. Lopes-Ferreira C. A. O. Ricart M. V. Souza F. Hasselman-Zielinski J. A. Becker L. F. Pereira |
author_sort |
S. L. Elífio-Esposito |
title |
A C-type lectin from Bothrops jararacussu venom can adhere to extracellular matrix proteins and induce the rolling of leukocytes |
title_short |
A C-type lectin from Bothrops jararacussu venom can adhere to extracellular matrix proteins and induce the rolling of leukocytes |
title_full |
A C-type lectin from Bothrops jararacussu venom can adhere to extracellular matrix proteins and induce the rolling of leukocytes |
title_fullStr |
A C-type lectin from Bothrops jararacussu venom can adhere to extracellular matrix proteins and induce the rolling of leukocytes |
title_full_unstemmed |
A C-type lectin from Bothrops jararacussu venom can adhere to extracellular matrix proteins and induce the rolling of leukocytes |
title_sort |
c-type lectin from bothrops jararacussu venom can adhere to extracellular matrix proteins and induce the rolling of leukocytes |
publisher |
SciELO |
publishDate |
2007 |
url |
https://doi.org/10.1590/S1678-91992007000400009 https://doaj.org/article/5aeed971530247a2b933c4b3f9fab6e8 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 13, Iss 4, Pp 782-799 (2007) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400009 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992007000400009 1678-9199 https://doaj.org/article/5aeed971530247a2b933c4b3f9fab6e8 |
op_doi |
https://doi.org/10.1590/S1678-91992007000400009 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
13 |
container_issue |
4 |
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1766341449766404096 |