Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from Artocarpus heterophyllus heartwoods
Objective: To evaluate the immunosuppressive effect on human phagocytes and antibacterial activity of dihydromorin and norartocarpetin isolated from Artocarpus heterophyllus heartwoods. Methods: Dihydromorin and norartocarpetin were isolated from Artocarpus heterophyllus heartwoods. A modified Boyde...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2020
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Online Access: | https://doi.org/10.4103/2221-1691.287162 https://doaj.org/article/5ad0fc8308d645838a1d3cc061e26b7b |
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ftdoajarticles:oai:doaj.org/article:5ad0fc8308d645838a1d3cc061e26b7b 2023-05-15T15:08:44+02:00 Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from Artocarpus heterophyllus heartwoods Abdi Wira Septama Ibrahim Jantan Pharkphoom Panichayupakaranant Mohd Fadhlizil Fasihi Mohd Aluwi Eldiza Puji Rahmi 2020-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.287162 https://doaj.org/article/5ad0fc8308d645838a1d3cc061e26b7b EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=8;spage=361;epage=368;aulast=Septama https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.287162 https://doaj.org/article/5ad0fc8308d645838a1d3cc061e26b7b Asian Pacific Journal of Tropical Biomedicine, Vol 10, Iss 8, Pp 361-368 (2020) dihydromorin immunosuppressive antibacterial flavonoid myeloperoxidase artocarpus heterophyllus Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2020 ftdoajarticles https://doi.org/10.4103/2221-1691.287162 2022-12-31T03:15:44Z Objective: To evaluate the immunosuppressive effect on human phagocytes and antibacterial activity of dihydromorin and norartocarpetin isolated from Artocarpus heterophyllus heartwoods. Methods: Dihydromorin and norartocarpetin were isolated from Artocarpus heterophyllus heartwoods. A modified Boyden chamber was used to determine the chemotactic activity of human phagocyte. The respiratory burst was evaluated by chemiluminescence assay. Myeloperoxidase (MPO) activity was quantified using a colorimetric assay. The broth microdilution method was performed to assess their antibacterial activity. Results: Dihydromorin exhibited potent inhibitory effect on the chemotactic activity of polymorphonuclear neutrophils (PMNs) with an IC50 value of 5.03 μg/mL. Dihydromorin also inhibited reactive oxygen species production of whole blood cells, PMNs, and monocytes with IC50 values of 7.88, 7.59 and 7.24 μg/mL, respectively. Interestingly, dihydromorin also strongly inhibited the MPO activity of PMNs with an IC50 value of 5.24 μg/mL, which was lower than indomethacin (24.6 μg/mL). Molecular docking of dihydromorin and crystal structure of MPO showed that dihydromorin had close interaction with key amino acid residues such as Arg239 and Gln91. Antibacterial activity assay showed that only dihydromorin had a strong effect against Streptococcus pyogenes with MIC and MBC values of 15.62 and 31.25 μg/mL, respectively. Conclusions: The results suggest that dihydromorin could be developed as an anti-inflammatory and antibacterial agent. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 10 8 361 |
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ftdoajarticles |
language |
English |
topic |
dihydromorin immunosuppressive antibacterial flavonoid myeloperoxidase artocarpus heterophyllus Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
dihydromorin immunosuppressive antibacterial flavonoid myeloperoxidase artocarpus heterophyllus Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Abdi Wira Septama Ibrahim Jantan Pharkphoom Panichayupakaranant Mohd Fadhlizil Fasihi Mohd Aluwi Eldiza Puji Rahmi Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from Artocarpus heterophyllus heartwoods |
topic_facet |
dihydromorin immunosuppressive antibacterial flavonoid myeloperoxidase artocarpus heterophyllus Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To evaluate the immunosuppressive effect on human phagocytes and antibacterial activity of dihydromorin and norartocarpetin isolated from Artocarpus heterophyllus heartwoods. Methods: Dihydromorin and norartocarpetin were isolated from Artocarpus heterophyllus heartwoods. A modified Boyden chamber was used to determine the chemotactic activity of human phagocyte. The respiratory burst was evaluated by chemiluminescence assay. Myeloperoxidase (MPO) activity was quantified using a colorimetric assay. The broth microdilution method was performed to assess their antibacterial activity. Results: Dihydromorin exhibited potent inhibitory effect on the chemotactic activity of polymorphonuclear neutrophils (PMNs) with an IC50 value of 5.03 μg/mL. Dihydromorin also inhibited reactive oxygen species production of whole blood cells, PMNs, and monocytes with IC50 values of 7.88, 7.59 and 7.24 μg/mL, respectively. Interestingly, dihydromorin also strongly inhibited the MPO activity of PMNs with an IC50 value of 5.24 μg/mL, which was lower than indomethacin (24.6 μg/mL). Molecular docking of dihydromorin and crystal structure of MPO showed that dihydromorin had close interaction with key amino acid residues such as Arg239 and Gln91. Antibacterial activity assay showed that only dihydromorin had a strong effect against Streptococcus pyogenes with MIC and MBC values of 15.62 and 31.25 μg/mL, respectively. Conclusions: The results suggest that dihydromorin could be developed as an anti-inflammatory and antibacterial agent. |
format |
Article in Journal/Newspaper |
author |
Abdi Wira Septama Ibrahim Jantan Pharkphoom Panichayupakaranant Mohd Fadhlizil Fasihi Mohd Aluwi Eldiza Puji Rahmi |
author_facet |
Abdi Wira Septama Ibrahim Jantan Pharkphoom Panichayupakaranant Mohd Fadhlizil Fasihi Mohd Aluwi Eldiza Puji Rahmi |
author_sort |
Abdi Wira Septama |
title |
Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from Artocarpus heterophyllus heartwoods |
title_short |
Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from Artocarpus heterophyllus heartwoods |
title_full |
Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from Artocarpus heterophyllus heartwoods |
title_fullStr |
Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from Artocarpus heterophyllus heartwoods |
title_full_unstemmed |
Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from Artocarpus heterophyllus heartwoods |
title_sort |
immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from artocarpus heterophyllus heartwoods |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2020 |
url |
https://doi.org/10.4103/2221-1691.287162 https://doaj.org/article/5ad0fc8308d645838a1d3cc061e26b7b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 10, Iss 8, Pp 361-368 (2020) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=8;spage=361;epage=368;aulast=Septama https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.287162 https://doaj.org/article/5ad0fc8308d645838a1d3cc061e26b7b |
op_doi |
https://doi.org/10.4103/2221-1691.287162 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
10 |
container_issue |
8 |
container_start_page |
361 |
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1766340037872451584 |