The hidden Plasmodium malariae in blood donors: a risk coming from areas of low transmission of malaria
ABSTRACT Malaria is an infectious vector-borne disease with other important routes of transmission, such as blood transfusion and organ/tissue transplantation, due to asymptomatic reservoirs of Plasmodium presenting with low parasitemia. Reports of transfusion-transmitted malaria have shown that in...
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ftdoajarticles:oai:doaj.org/article:5aaceca9a5dd40f497d2e0c3ef6817c7 2024-09-09T19:27:32+00:00 The hidden Plasmodium malariae in blood donors: a risk coming from areas of low transmission of malaria Mariana Aschar José Eduardo Levi Maria L. R. N. Farinas Sandra C. Montebello Alfredo Mendrone-Junior Silvia Maria Di Santi 2020-12-01T00:00:00Z https://doi.org/10.1590/s1678-9946202062100 https://doaj.org/article/5aaceca9a5dd40f497d2e0c3ef6817c7 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652020000100615&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946202062100 https://doaj.org/article/5aaceca9a5dd40f497d2e0c3ef6817c7 Revista do Instituto de Medicina Tropical de São Paulo, Vol 62 (2020) Malaria Molecular diagnosis Transfusion-transmitted malaria Plasmodium malariae Blood banks Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2020 ftdoajarticles https://doi.org/10.1590/s1678-9946202062100 2024-08-05T17:49:30Z ABSTRACT Malaria is an infectious vector-borne disease with other important routes of transmission, such as blood transfusion and organ/tissue transplantation, due to asymptomatic reservoirs of Plasmodium presenting with low parasitemia. Reports of transfusion-transmitted malaria have shown that in immunosuppressed recipients, infections can be fatal if they are not diagnosed and timely treated. All Plasmodium species can survive on blood components at temperatures from 2 to 6 °C for some days or even weeks. This report describes two candidates for blood donation harboring Plasmodium, infected in an area considered non-endemic. Blood samples were collected from donors who attended a blood bank in Sao Paulo and tested by microscopy, qPCR for Plasmodium genus-specific amplification, targeting the parasite 18S ribosomal subunit gene and a multiplex qPCR based on mtDNA of the five species. Under microscopy, only structures resembling Plasmodium were observed. The qPCR whose standard curve tested parasites varying from 2 to 0.1 parasites/ µL, showed the presence of Plasmodium DNA in the two blood donors, as did the multiplex qPCR that revealed the presence of P. malariae. The prevalence of positive donors varies according to the level of transmission, ranging from 0.7 to 55% in endemic areas. In non-endemic regions, prevalences are lower, however, transfusion malaria can evolve to severe cases, due to the lack of suspicion of this transmission route. Asymptomatic donors from low transmission regions pose a risk to blood banks, with particular emphasis on those located in areas with malaria elimination goals. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 62 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Malaria Molecular diagnosis Transfusion-transmitted malaria Plasmodium malariae Blood banks Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Malaria Molecular diagnosis Transfusion-transmitted malaria Plasmodium malariae Blood banks Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Mariana Aschar José Eduardo Levi Maria L. R. N. Farinas Sandra C. Montebello Alfredo Mendrone-Junior Silvia Maria Di Santi The hidden Plasmodium malariae in blood donors: a risk coming from areas of low transmission of malaria |
topic_facet |
Malaria Molecular diagnosis Transfusion-transmitted malaria Plasmodium malariae Blood banks Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
ABSTRACT Malaria is an infectious vector-borne disease with other important routes of transmission, such as blood transfusion and organ/tissue transplantation, due to asymptomatic reservoirs of Plasmodium presenting with low parasitemia. Reports of transfusion-transmitted malaria have shown that in immunosuppressed recipients, infections can be fatal if they are not diagnosed and timely treated. All Plasmodium species can survive on blood components at temperatures from 2 to 6 °C for some days or even weeks. This report describes two candidates for blood donation harboring Plasmodium, infected in an area considered non-endemic. Blood samples were collected from donors who attended a blood bank in Sao Paulo and tested by microscopy, qPCR for Plasmodium genus-specific amplification, targeting the parasite 18S ribosomal subunit gene and a multiplex qPCR based on mtDNA of the five species. Under microscopy, only structures resembling Plasmodium were observed. The qPCR whose standard curve tested parasites varying from 2 to 0.1 parasites/ µL, showed the presence of Plasmodium DNA in the two blood donors, as did the multiplex qPCR that revealed the presence of P. malariae. The prevalence of positive donors varies according to the level of transmission, ranging from 0.7 to 55% in endemic areas. In non-endemic regions, prevalences are lower, however, transfusion malaria can evolve to severe cases, due to the lack of suspicion of this transmission route. Asymptomatic donors from low transmission regions pose a risk to blood banks, with particular emphasis on those located in areas with malaria elimination goals. |
format |
Article in Journal/Newspaper |
author |
Mariana Aschar José Eduardo Levi Maria L. R. N. Farinas Sandra C. Montebello Alfredo Mendrone-Junior Silvia Maria Di Santi |
author_facet |
Mariana Aschar José Eduardo Levi Maria L. R. N. Farinas Sandra C. Montebello Alfredo Mendrone-Junior Silvia Maria Di Santi |
author_sort |
Mariana Aschar |
title |
The hidden Plasmodium malariae in blood donors: a risk coming from areas of low transmission of malaria |
title_short |
The hidden Plasmodium malariae in blood donors: a risk coming from areas of low transmission of malaria |
title_full |
The hidden Plasmodium malariae in blood donors: a risk coming from areas of low transmission of malaria |
title_fullStr |
The hidden Plasmodium malariae in blood donors: a risk coming from areas of low transmission of malaria |
title_full_unstemmed |
The hidden Plasmodium malariae in blood donors: a risk coming from areas of low transmission of malaria |
title_sort |
hidden plasmodium malariae in blood donors: a risk coming from areas of low transmission of malaria |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2020 |
url |
https://doi.org/10.1590/s1678-9946202062100 https://doaj.org/article/5aaceca9a5dd40f497d2e0c3ef6817c7 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 62 (2020) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652020000100615&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946202062100 https://doaj.org/article/5aaceca9a5dd40f497d2e0c3ef6817c7 |
op_doi |
https://doi.org/10.1590/s1678-9946202062100 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
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62 |
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