Cytotoxic activity of crude extracts and fractions from Premna odorata (Blanco), Artocarpus camansi (Blanco) and Gliricidia sepium (Jacq.) against selected human cancer cell lines
Objective: To evaluate the cytotoxic activities of Premna odorata (P. odorata) leaves and bark, Artocarpus camansi (A. camansi) and Gliricidia sepium against selected human cancer cell lines by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Methods: The crude extract...
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Wolters Kluwer Medknow Publications
2015
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Online Access: | https://doi.org/10.1016/j.apjtb.2015.09.011 https://doaj.org/article/5aa740223c974bed919c28b2e1e8f325 |
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ftdoajarticles:oai:doaj.org/article:5aa740223c974bed919c28b2e1e8f325 2023-05-15T15:08:23+02:00 Cytotoxic activity of crude extracts and fractions from Premna odorata (Blanco), Artocarpus camansi (Blanco) and Gliricidia sepium (Jacq.) against selected human cancer cell lines Ourlad Alzeus G. Tantengco Sonia D. Jacinto 2015-12-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2015.09.011 https://doaj.org/article/5aa740223c974bed919c28b2e1e8f325 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115002233 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2015.09.011 https://doaj.org/article/5aa740223c974bed919c28b2e1e8f325 Asian Pacific Journal of Tropical Biomedicine, Vol 5, Iss 12, Pp 1037-1041 (2015) Cancer Cytotoxicity assay Premna odorata Artocarpus camansi Gliricidia sepium Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2015 ftdoajarticles https://doi.org/10.1016/j.apjtb.2015.09.011 2022-12-31T05:50:59Z Objective: To evaluate the cytotoxic activities of Premna odorata (P. odorata) leaves and bark, Artocarpus camansi (A. camansi) and Gliricidia sepium against selected human cancer cell lines by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Methods: The crude extracts of P. odorata, A. camansi and Gliricidia sepium were subjected to liquid–liquid partitioning by using hexane and ethyl acetate to separate compounds based on their polarity. The fractions were tested for their cytotoxic activity against human colon cancer cell line (HCT116), breast cancer cell line (MCF-7), lung adenocarcinoma cell line (A549) and Chinese hamster ovary cell line (AA8) by using MTT assay. Results: Based on the standard values of toxicity set by the study of Suffness and Pezzuto, P. odorata leaves and P. odorata bark hexane fractions and A. camansi leaves were all considered highly cytotoxic against the selected human cancer cell lines. P. odorata bark hexane extract exhibited the highest selectivity index for HCT116, MCF-7 and A549 cancer cell lines. Conclusions: The results obtained indicated that P. odorata leaves and bark and A. camansi leaves have excellent cytotoxic activity and warrant further studies to isolate novel compounds for chemotherapeutic use. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Blanco ENVELOPE(-55.233,-55.233,-61.250,-61.250) Asian Pacific Journal of Tropical Biomedicine 5 12 1037 1041 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Cancer Cytotoxicity assay Premna odorata Artocarpus camansi Gliricidia sepium Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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Cancer Cytotoxicity assay Premna odorata Artocarpus camansi Gliricidia sepium Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Ourlad Alzeus G. Tantengco Sonia D. Jacinto Cytotoxic activity of crude extracts and fractions from Premna odorata (Blanco), Artocarpus camansi (Blanco) and Gliricidia sepium (Jacq.) against selected human cancer cell lines |
topic_facet |
Cancer Cytotoxicity assay Premna odorata Artocarpus camansi Gliricidia sepium Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To evaluate the cytotoxic activities of Premna odorata (P. odorata) leaves and bark, Artocarpus camansi (A. camansi) and Gliricidia sepium against selected human cancer cell lines by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Methods: The crude extracts of P. odorata, A. camansi and Gliricidia sepium were subjected to liquid–liquid partitioning by using hexane and ethyl acetate to separate compounds based on their polarity. The fractions were tested for their cytotoxic activity against human colon cancer cell line (HCT116), breast cancer cell line (MCF-7), lung adenocarcinoma cell line (A549) and Chinese hamster ovary cell line (AA8) by using MTT assay. Results: Based on the standard values of toxicity set by the study of Suffness and Pezzuto, P. odorata leaves and P. odorata bark hexane fractions and A. camansi leaves were all considered highly cytotoxic against the selected human cancer cell lines. P. odorata bark hexane extract exhibited the highest selectivity index for HCT116, MCF-7 and A549 cancer cell lines. Conclusions: The results obtained indicated that P. odorata leaves and bark and A. camansi leaves have excellent cytotoxic activity and warrant further studies to isolate novel compounds for chemotherapeutic use. |
format |
Article in Journal/Newspaper |
author |
Ourlad Alzeus G. Tantengco Sonia D. Jacinto |
author_facet |
Ourlad Alzeus G. Tantengco Sonia D. Jacinto |
author_sort |
Ourlad Alzeus G. Tantengco |
title |
Cytotoxic activity of crude extracts and fractions from Premna odorata (Blanco), Artocarpus camansi (Blanco) and Gliricidia sepium (Jacq.) against selected human cancer cell lines |
title_short |
Cytotoxic activity of crude extracts and fractions from Premna odorata (Blanco), Artocarpus camansi (Blanco) and Gliricidia sepium (Jacq.) against selected human cancer cell lines |
title_full |
Cytotoxic activity of crude extracts and fractions from Premna odorata (Blanco), Artocarpus camansi (Blanco) and Gliricidia sepium (Jacq.) against selected human cancer cell lines |
title_fullStr |
Cytotoxic activity of crude extracts and fractions from Premna odorata (Blanco), Artocarpus camansi (Blanco) and Gliricidia sepium (Jacq.) against selected human cancer cell lines |
title_full_unstemmed |
Cytotoxic activity of crude extracts and fractions from Premna odorata (Blanco), Artocarpus camansi (Blanco) and Gliricidia sepium (Jacq.) against selected human cancer cell lines |
title_sort |
cytotoxic activity of crude extracts and fractions from premna odorata (blanco), artocarpus camansi (blanco) and gliricidia sepium (jacq.) against selected human cancer cell lines |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2015 |
url |
https://doi.org/10.1016/j.apjtb.2015.09.011 https://doaj.org/article/5aa740223c974bed919c28b2e1e8f325 |
long_lat |
ENVELOPE(-55.233,-55.233,-61.250,-61.250) |
geographic |
Arctic Blanco |
geographic_facet |
Arctic Blanco |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 5, Iss 12, Pp 1037-1041 (2015) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115002233 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2015.09.011 https://doaj.org/article/5aa740223c974bed919c28b2e1e8f325 |
op_doi |
https://doi.org/10.1016/j.apjtb.2015.09.011 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
5 |
container_issue |
12 |
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1037 |
op_container_end_page |
1041 |
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1766339753826844672 |