A transcriptomic survey of the impact of environmental stress on response to dengue virus in the mosquito, Aedes aegypti.
Populations of Aedes aegypti naturally exhibit variable susceptibility to dengue viruses. This natural variation can be impacted by nutritional stress resulting from larval-stage crowding, indicating the influence of environment components on the adult mosquito immune response. In particular, larval...
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ftdoajarticles:oai:doaj.org/article:5a7281efc8b24b359b485ad1d6d2dac1 2023-05-15T15:08:57+02:00 A transcriptomic survey of the impact of environmental stress on response to dengue virus in the mosquito, Aedes aegypti. David S Kang Martin S Barron Diane D Lovin Joanne M Cunningham Matthew W Eng Dave D Chadee Jun Li David W Severson 2018-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006568 https://doaj.org/article/5a7281efc8b24b359b485ad1d6d2dac1 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC6013235?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006568 https://doaj.org/article/5a7281efc8b24b359b485ad1d6d2dac1 PLoS Neglected Tropical Diseases, Vol 12, Iss 6, p e0006568 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006568 2022-12-31T00:20:15Z Populations of Aedes aegypti naturally exhibit variable susceptibility to dengue viruses. This natural variation can be impacted by nutritional stress resulting from larval-stage crowding, indicating the influence of environment components on the adult mosquito immune response. In particular, larval crowding was previously shown to reduce the susceptibility of adult females of a Trinidad field isolate of A. aegypti to the dengue serotype 2 (JAM1409) virus. Here, we present the first whole transcriptome study to address the impact of environmental stress on A. aegypti response to dengue virus. We examined expression profiles of adult females resulting from crowded and optimum reared larvae from the same Trinidad isolate at two critical early time points-3 and 18 hours post dengue virus infected blood meal. We exposed specimens to either a dengue or naïve blood meal, and then characterized the response in ten gene co-expression modules based on their transcriptional associations with environmental stress and time. We further analyzed the top 30 hub or master regulatory genes in each of the modules, and validated our results via qRT-PCR. These hub genes reveal which functions are critical to the mechanisms that confer dengue virus refractoriness or susceptibility to stress conditioned A. aegypti, as well as the time points at which they are most important. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Trinidad ENVELOPE(-60.734,-60.734,-63.816,-63.816) PLOS Neglected Tropical Diseases 12 6 e0006568 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 David S Kang Martin S Barron Diane D Lovin Joanne M Cunningham Matthew W Eng Dave D Chadee Jun Li David W Severson A transcriptomic survey of the impact of environmental stress on response to dengue virus in the mosquito, Aedes aegypti. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Populations of Aedes aegypti naturally exhibit variable susceptibility to dengue viruses. This natural variation can be impacted by nutritional stress resulting from larval-stage crowding, indicating the influence of environment components on the adult mosquito immune response. In particular, larval crowding was previously shown to reduce the susceptibility of adult females of a Trinidad field isolate of A. aegypti to the dengue serotype 2 (JAM1409) virus. Here, we present the first whole transcriptome study to address the impact of environmental stress on A. aegypti response to dengue virus. We examined expression profiles of adult females resulting from crowded and optimum reared larvae from the same Trinidad isolate at two critical early time points-3 and 18 hours post dengue virus infected blood meal. We exposed specimens to either a dengue or naïve blood meal, and then characterized the response in ten gene co-expression modules based on their transcriptional associations with environmental stress and time. We further analyzed the top 30 hub or master regulatory genes in each of the modules, and validated our results via qRT-PCR. These hub genes reveal which functions are critical to the mechanisms that confer dengue virus refractoriness or susceptibility to stress conditioned A. aegypti, as well as the time points at which they are most important. |
format |
Article in Journal/Newspaper |
author |
David S Kang Martin S Barron Diane D Lovin Joanne M Cunningham Matthew W Eng Dave D Chadee Jun Li David W Severson |
author_facet |
David S Kang Martin S Barron Diane D Lovin Joanne M Cunningham Matthew W Eng Dave D Chadee Jun Li David W Severson |
author_sort |
David S Kang |
title |
A transcriptomic survey of the impact of environmental stress on response to dengue virus in the mosquito, Aedes aegypti. |
title_short |
A transcriptomic survey of the impact of environmental stress on response to dengue virus in the mosquito, Aedes aegypti. |
title_full |
A transcriptomic survey of the impact of environmental stress on response to dengue virus in the mosquito, Aedes aegypti. |
title_fullStr |
A transcriptomic survey of the impact of environmental stress on response to dengue virus in the mosquito, Aedes aegypti. |
title_full_unstemmed |
A transcriptomic survey of the impact of environmental stress on response to dengue virus in the mosquito, Aedes aegypti. |
title_sort |
transcriptomic survey of the impact of environmental stress on response to dengue virus in the mosquito, aedes aegypti. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0006568 https://doaj.org/article/5a7281efc8b24b359b485ad1d6d2dac1 |
long_lat |
ENVELOPE(-60.734,-60.734,-63.816,-63.816) |
geographic |
Arctic Trinidad |
geographic_facet |
Arctic Trinidad |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 6, p e0006568 (2018) |
op_relation |
http://europepmc.org/articles/PMC6013235?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006568 https://doaj.org/article/5a7281efc8b24b359b485ad1d6d2dac1 |
op_doi |
https://doi.org/10.1371/journal.pntd.0006568 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
12 |
container_issue |
6 |
container_start_page |
e0006568 |
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1766340214202040320 |