Purification, Identification, Activity Evaluation, and Stability of Antioxidant Peptides from Alcalase Hydrolysate of Antarctic Krill ( Euphausia superba ) Proteins
For utilizing the largest source of marine proteins, Antarctic krill ( Euphausia superba ) proteins were defatted and hydrolyzed separately using pepsin, alcalase, papain, trypsin, and netrase, and alcalase hydrolysate (EPAH) showed the highest DPPH radical (DPPH·) and hydroxyl radical (HO·) scaveng...
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ftdoajarticles:oai:doaj.org/article:59fdb9e343dd4aaf9b3a1d212240a0c3 2023-05-15T13:52:23+02:00 Purification, Identification, Activity Evaluation, and Stability of Antioxidant Peptides from Alcalase Hydrolysate of Antarctic Krill ( Euphausia superba ) Proteins Shuang-Yi Zhang Guo-Xu Zhao Shi-Kun Suo Yu-Mei Wang Chang-Feng Chi Bin Wang 2021-06-01T00:00:00Z https://doi.org/10.3390/md19060347 https://doaj.org/article/59fdb9e343dd4aaf9b3a1d212240a0c3 EN eng MDPI AG https://www.mdpi.com/1660-3397/19/6/347 https://doaj.org/toc/1660-3397 doi:10.3390/md19060347 1660-3397 https://doaj.org/article/59fdb9e343dd4aaf9b3a1d212240a0c3 Marine Drugs, Vol 19, Iss 347, p 347 (2021) Antarctic krill ( Euphausia superba ) peptide antioxidant activity stability Biology (General) QH301-705.5 article 2021 ftdoajarticles https://doi.org/10.3390/md19060347 2022-12-30T19:50:09Z For utilizing the largest source of marine proteins, Antarctic krill ( Euphausia superba ) proteins were defatted and hydrolyzed separately using pepsin, alcalase, papain, trypsin, and netrase, and alcalase hydrolysate (EPAH) showed the highest DPPH radical (DPPH·) and hydroxyl radical (HO·) scavenging activity among five hydrolysates. Using ultrafiltration and chromatography methods, fifteen antioxidant peptides were purified from EPAH and identified as Asn-Gln-Met (NQM), Trp-Phe-Pro-Met (WFPM), Gln-Asn-Pro-Thr (QNPT), Tyr-Met-Asn-Phe (YMNF), Ser-Gly-Pro-Ala (SGPA), Ser-Leu-Pro-Tyr (SLPY), Gln-Tyr-Pro-Pro-Met-Gln-Tyr (QYPPMQY), Glu-Tyr-Glu-Ala (EYEA), Asn-Trp-Asp-Asp-Met-Arg-Ile-Val-Ala-Val (NWDDMRIVAV), Trp-Asp-Asp-Met-Glu-Arg-Leu-Val-Met-Ile (WDDMERLVMI), Asn-Trp-Asp-Asp-Met-Glu-Pro-Ser-Phe (NWD-DMEPSF), Asn-Gly-Pro-Asp-Pro-Arg-Pro-Ser-Gln-Gln (NGPDPRPSQQ), Ala-Phe-Leu-Trp-Asn (AFLWA), Asn-Val-Pro-Asp-Met (NVPDM), and Thr-Phe-Pro-Ile-Tyr-Asp-Tyr-Pro-Gln (TFPIYDPQ), respectively, using a protein sequencer and ESI/MS. Among fifteen antioxidant peptides, SLPY, QYPPMQY and EYEA showed the highest scavenging activities on DPPH· (EC 50 values of 1.18 ± 0.036, 1.547 ± 0.150, and 1.372 ± 0.274 mg/mL, respectively), HO· (EC 50 values of 0.826 ± 0.027, 1.022 ± 0.058, and 0.946 ± 0.011 mg/mL, respectively), and superoxide anion radical (EC 50 values of 0.789 ± 0.079, 0.913 ± 0.007, and 0.793 ± 0.056 mg/mL, respectively). Moreover, SLPY, QYPPMQY and EYEA showed strong reducing power, protective capability against H 2 O 2 -damaged plasmid DNA, and lipid peroxidation inhibition ability. Furthermore, SLPY, QYPPMQY, and EYEA had high stability under temperatures lower than 80 °C, pH values ranged from 6–8, and simulated GI digestion for 180 min. The results showed that fifteen antioxidant peptides from alcalase hydrolysate of Antarctic krill proteins, especially SLPY, QYPPMQY and EYEA, might serve as effective antioxidant agents applied in food and health products. Article in Journal/Newspaper Antarc* Antarctic Antarctic Krill Euphausia superba Directory of Open Access Journals: DOAJ Articles Antarctic Marine Drugs 19 6 347 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antarctic krill ( Euphausia superba ) peptide antioxidant activity stability Biology (General) QH301-705.5 |
spellingShingle |
Antarctic krill ( Euphausia superba ) peptide antioxidant activity stability Biology (General) QH301-705.5 Shuang-Yi Zhang Guo-Xu Zhao Shi-Kun Suo Yu-Mei Wang Chang-Feng Chi Bin Wang Purification, Identification, Activity Evaluation, and Stability of Antioxidant Peptides from Alcalase Hydrolysate of Antarctic Krill ( Euphausia superba ) Proteins |
topic_facet |
Antarctic krill ( Euphausia superba ) peptide antioxidant activity stability Biology (General) QH301-705.5 |
description |
For utilizing the largest source of marine proteins, Antarctic krill ( Euphausia superba ) proteins were defatted and hydrolyzed separately using pepsin, alcalase, papain, trypsin, and netrase, and alcalase hydrolysate (EPAH) showed the highest DPPH radical (DPPH·) and hydroxyl radical (HO·) scavenging activity among five hydrolysates. Using ultrafiltration and chromatography methods, fifteen antioxidant peptides were purified from EPAH and identified as Asn-Gln-Met (NQM), Trp-Phe-Pro-Met (WFPM), Gln-Asn-Pro-Thr (QNPT), Tyr-Met-Asn-Phe (YMNF), Ser-Gly-Pro-Ala (SGPA), Ser-Leu-Pro-Tyr (SLPY), Gln-Tyr-Pro-Pro-Met-Gln-Tyr (QYPPMQY), Glu-Tyr-Glu-Ala (EYEA), Asn-Trp-Asp-Asp-Met-Arg-Ile-Val-Ala-Val (NWDDMRIVAV), Trp-Asp-Asp-Met-Glu-Arg-Leu-Val-Met-Ile (WDDMERLVMI), Asn-Trp-Asp-Asp-Met-Glu-Pro-Ser-Phe (NWD-DMEPSF), Asn-Gly-Pro-Asp-Pro-Arg-Pro-Ser-Gln-Gln (NGPDPRPSQQ), Ala-Phe-Leu-Trp-Asn (AFLWA), Asn-Val-Pro-Asp-Met (NVPDM), and Thr-Phe-Pro-Ile-Tyr-Asp-Tyr-Pro-Gln (TFPIYDPQ), respectively, using a protein sequencer and ESI/MS. Among fifteen antioxidant peptides, SLPY, QYPPMQY and EYEA showed the highest scavenging activities on DPPH· (EC 50 values of 1.18 ± 0.036, 1.547 ± 0.150, and 1.372 ± 0.274 mg/mL, respectively), HO· (EC 50 values of 0.826 ± 0.027, 1.022 ± 0.058, and 0.946 ± 0.011 mg/mL, respectively), and superoxide anion radical (EC 50 values of 0.789 ± 0.079, 0.913 ± 0.007, and 0.793 ± 0.056 mg/mL, respectively). Moreover, SLPY, QYPPMQY and EYEA showed strong reducing power, protective capability against H 2 O 2 -damaged plasmid DNA, and lipid peroxidation inhibition ability. Furthermore, SLPY, QYPPMQY, and EYEA had high stability under temperatures lower than 80 °C, pH values ranged from 6–8, and simulated GI digestion for 180 min. The results showed that fifteen antioxidant peptides from alcalase hydrolysate of Antarctic krill proteins, especially SLPY, QYPPMQY and EYEA, might serve as effective antioxidant agents applied in food and health products. |
format |
Article in Journal/Newspaper |
author |
Shuang-Yi Zhang Guo-Xu Zhao Shi-Kun Suo Yu-Mei Wang Chang-Feng Chi Bin Wang |
author_facet |
Shuang-Yi Zhang Guo-Xu Zhao Shi-Kun Suo Yu-Mei Wang Chang-Feng Chi Bin Wang |
author_sort |
Shuang-Yi Zhang |
title |
Purification, Identification, Activity Evaluation, and Stability of Antioxidant Peptides from Alcalase Hydrolysate of Antarctic Krill ( Euphausia superba ) Proteins |
title_short |
Purification, Identification, Activity Evaluation, and Stability of Antioxidant Peptides from Alcalase Hydrolysate of Antarctic Krill ( Euphausia superba ) Proteins |
title_full |
Purification, Identification, Activity Evaluation, and Stability of Antioxidant Peptides from Alcalase Hydrolysate of Antarctic Krill ( Euphausia superba ) Proteins |
title_fullStr |
Purification, Identification, Activity Evaluation, and Stability of Antioxidant Peptides from Alcalase Hydrolysate of Antarctic Krill ( Euphausia superba ) Proteins |
title_full_unstemmed |
Purification, Identification, Activity Evaluation, and Stability of Antioxidant Peptides from Alcalase Hydrolysate of Antarctic Krill ( Euphausia superba ) Proteins |
title_sort |
purification, identification, activity evaluation, and stability of antioxidant peptides from alcalase hydrolysate of antarctic krill ( euphausia superba ) proteins |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/md19060347 https://doaj.org/article/59fdb9e343dd4aaf9b3a1d212240a0c3 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctic Krill Euphausia superba |
genre_facet |
Antarc* Antarctic Antarctic Krill Euphausia superba |
op_source |
Marine Drugs, Vol 19, Iss 347, p 347 (2021) |
op_relation |
https://www.mdpi.com/1660-3397/19/6/347 https://doaj.org/toc/1660-3397 doi:10.3390/md19060347 1660-3397 https://doaj.org/article/59fdb9e343dd4aaf9b3a1d212240a0c3 |
op_doi |
https://doi.org/10.3390/md19060347 |
container_title |
Marine Drugs |
container_volume |
19 |
container_issue |
6 |
container_start_page |
347 |
_version_ |
1766256668300017664 |