Biochemical Characterization of a Carrageenase, Car1383, Derived From Associated Bacteria of Antarctic Macroalgae
A carrageenase gene, car1383, was obtained from the metagenome of Antarctic macroalgae-associated bacteria. The amino acid sequence of its product showed up to 33% similarity with other carrageenases and contained a GH16-family motif. The recombinant Car1383 was heterologously expressed in Escherici...
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ftdoajarticles:oai:doaj.org/article:59edea3c3ecd48deb320ebd3af5278ed 2023-05-15T13:38:23+02:00 Biochemical Characterization of a Carrageenase, Car1383, Derived From Associated Bacteria of Antarctic Macroalgae Jiang Li Xiaoqian Gu Qian Zhang Liping Fu Jiaojiao Tan Luying Zhao 2022-03-01T00:00:00Z https://doi.org/10.3389/fmicb.2022.851182 https://doaj.org/article/59edea3c3ecd48deb320ebd3af5278ed EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fmicb.2022.851182/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2022.851182 https://doaj.org/article/59edea3c3ecd48deb320ebd3af5278ed Frontiers in Microbiology, Vol 13 (2022) carrageenase Antarctic macroalgae associated bacteria metagenome site-directed mutagenesis Microbiology QR1-502 article 2022 ftdoajarticles https://doi.org/10.3389/fmicb.2022.851182 2022-12-31T03:53:52Z A carrageenase gene, car1383, was obtained from the metagenome of Antarctic macroalgae-associated bacteria. The amino acid sequence of its product showed up to 33% similarity with other carrageenases and contained a GH16-family motif. The recombinant Car1383 was heterologously expressed in Eschericia coli and exhibited maximal activity at 50°C and pH 6.0, with a Km of 6.51 mg/ml and a Vmax of 55.77 U/mg. Its activity was enhanced by some cations (Na+, K+, and Fe2+), but inhibited or inactivated by others (Sr2+, Ca2+, Ni2+, Ba2+, Mn2+, Cu2+, Fe3+, and Mg2+). Car1383 degraded carrageenan into neocarrabiose and neocarratetraose. Site-directed mutagenesis indicated that putative active sites, E190 and E195, conserved sites, W183 and G255, play important roles in Car1383 activity. This study provides a new candidate for the industrial preparation of bioactive algal oligosaccharides. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic Frontiers in Microbiology 13 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
carrageenase Antarctic macroalgae associated bacteria metagenome site-directed mutagenesis Microbiology QR1-502 |
spellingShingle |
carrageenase Antarctic macroalgae associated bacteria metagenome site-directed mutagenesis Microbiology QR1-502 Jiang Li Xiaoqian Gu Qian Zhang Liping Fu Jiaojiao Tan Luying Zhao Biochemical Characterization of a Carrageenase, Car1383, Derived From Associated Bacteria of Antarctic Macroalgae |
topic_facet |
carrageenase Antarctic macroalgae associated bacteria metagenome site-directed mutagenesis Microbiology QR1-502 |
description |
A carrageenase gene, car1383, was obtained from the metagenome of Antarctic macroalgae-associated bacteria. The amino acid sequence of its product showed up to 33% similarity with other carrageenases and contained a GH16-family motif. The recombinant Car1383 was heterologously expressed in Eschericia coli and exhibited maximal activity at 50°C and pH 6.0, with a Km of 6.51 mg/ml and a Vmax of 55.77 U/mg. Its activity was enhanced by some cations (Na+, K+, and Fe2+), but inhibited or inactivated by others (Sr2+, Ca2+, Ni2+, Ba2+, Mn2+, Cu2+, Fe3+, and Mg2+). Car1383 degraded carrageenan into neocarrabiose and neocarratetraose. Site-directed mutagenesis indicated that putative active sites, E190 and E195, conserved sites, W183 and G255, play important roles in Car1383 activity. This study provides a new candidate for the industrial preparation of bioactive algal oligosaccharides. |
format |
Article in Journal/Newspaper |
author |
Jiang Li Xiaoqian Gu Qian Zhang Liping Fu Jiaojiao Tan Luying Zhao |
author_facet |
Jiang Li Xiaoqian Gu Qian Zhang Liping Fu Jiaojiao Tan Luying Zhao |
author_sort |
Jiang Li |
title |
Biochemical Characterization of a Carrageenase, Car1383, Derived From Associated Bacteria of Antarctic Macroalgae |
title_short |
Biochemical Characterization of a Carrageenase, Car1383, Derived From Associated Bacteria of Antarctic Macroalgae |
title_full |
Biochemical Characterization of a Carrageenase, Car1383, Derived From Associated Bacteria of Antarctic Macroalgae |
title_fullStr |
Biochemical Characterization of a Carrageenase, Car1383, Derived From Associated Bacteria of Antarctic Macroalgae |
title_full_unstemmed |
Biochemical Characterization of a Carrageenase, Car1383, Derived From Associated Bacteria of Antarctic Macroalgae |
title_sort |
biochemical characterization of a carrageenase, car1383, derived from associated bacteria of antarctic macroalgae |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
https://doi.org/10.3389/fmicb.2022.851182 https://doaj.org/article/59edea3c3ecd48deb320ebd3af5278ed |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Frontiers in Microbiology, Vol 13 (2022) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fmicb.2022.851182/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2022.851182 https://doaj.org/article/59edea3c3ecd48deb320ebd3af5278ed |
op_doi |
https://doi.org/10.3389/fmicb.2022.851182 |
container_title |
Frontiers in Microbiology |
container_volume |
13 |
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1766105289707225088 |