Cost-effectiveness of blood agar for isolation of mycobacteria.
BACKGROUND: Mycobacterium species are grown using specific media that increase laboratory cost, thus hampering their diffusion in resource-limited countries. Preliminary data suggested that versatile blood agar may be also used for mycobacterial culture. METHODOLOGY: We examined the growth of 41 dif...
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ftdoajarticles:oai:doaj.org/article:59d4a39040354321a70b34b381b48dd3 2023-05-15T15:12:09+02:00 Cost-effectiveness of blood agar for isolation of mycobacteria. Michel Drancourt Didier Raoult 2007-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000083 https://doaj.org/article/59d4a39040354321a70b34b381b48dd3 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2100370?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0000083 https://doaj.org/article/59d4a39040354321a70b34b381b48dd3 PLoS Neglected Tropical Diseases, Vol 1, Iss 2, p e83 (2007) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2007 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000083 2022-12-31T15:58:41Z BACKGROUND: Mycobacterium species are grown using specific media that increase laboratory cost, thus hampering their diffusion in resource-limited countries. Preliminary data suggested that versatile blood agar may be also used for mycobacterial culture. METHODOLOGY: We examined the growth of 41 different Mycobacterium species on 5% blood agar. Over a 24-month period we analysed isolation of mycobacteria after parallel inoculation of clinical specimens into both a reference automated system (BACTEC 9000 MB broth) and 5% blood agar slant tubes, after NaOH decontamination, and compared the cost of performing 1,000 analyses using these two techniques. CONCLUSIONS: Mycobacterium reference species cultured on blood agar, with the exception of Mycobacterium ulcerans. Inoculation of 1,634 specimens yielded 95 Mycobacterium isolates. Blood agar performed significantly more efficiently than BACTEC 9000 MB broth (94 vs 88 isolates, P = 0.03). Decontamination of Candida albicans in 5 specimens by addition of amphotericin B in blood agar yielded one more M. tuberculosis isolate that could not be isolated in BACTEC broth. Uneven distribution of time to culture positivity for M. tuberculosis had a median (range) of 19+/-5 days using blood agar and 26+/-6 days using BACTEC 9000 MB broth. Cost for 1,000 analyses in France was estimated to be of 1,913 euros using the blood agar method and 8,990 euros using the BACTEC 9000 MB method. Blood agar should be regarded as a first-line medium for culturing Mycobacterium species. It saves time, is cost-effective, is more sensitive than, and at least as rapid as the automated method. This is of particular importance for resource-limited countries in which the prevalence of tuberculosis is high. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 1 2 e83 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Michel Drancourt Didier Raoult Cost-effectiveness of blood agar for isolation of mycobacteria. |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: Mycobacterium species are grown using specific media that increase laboratory cost, thus hampering their diffusion in resource-limited countries. Preliminary data suggested that versatile blood agar may be also used for mycobacterial culture. METHODOLOGY: We examined the growth of 41 different Mycobacterium species on 5% blood agar. Over a 24-month period we analysed isolation of mycobacteria after parallel inoculation of clinical specimens into both a reference automated system (BACTEC 9000 MB broth) and 5% blood agar slant tubes, after NaOH decontamination, and compared the cost of performing 1,000 analyses using these two techniques. CONCLUSIONS: Mycobacterium reference species cultured on blood agar, with the exception of Mycobacterium ulcerans. Inoculation of 1,634 specimens yielded 95 Mycobacterium isolates. Blood agar performed significantly more efficiently than BACTEC 9000 MB broth (94 vs 88 isolates, P = 0.03). Decontamination of Candida albicans in 5 specimens by addition of amphotericin B in blood agar yielded one more M. tuberculosis isolate that could not be isolated in BACTEC broth. Uneven distribution of time to culture positivity for M. tuberculosis had a median (range) of 19+/-5 days using blood agar and 26+/-6 days using BACTEC 9000 MB broth. Cost for 1,000 analyses in France was estimated to be of 1,913 euros using the blood agar method and 8,990 euros using the BACTEC 9000 MB method. Blood agar should be regarded as a first-line medium for culturing Mycobacterium species. It saves time, is cost-effective, is more sensitive than, and at least as rapid as the automated method. This is of particular importance for resource-limited countries in which the prevalence of tuberculosis is high. |
format |
Article in Journal/Newspaper |
author |
Michel Drancourt Didier Raoult |
author_facet |
Michel Drancourt Didier Raoult |
author_sort |
Michel Drancourt |
title |
Cost-effectiveness of blood agar for isolation of mycobacteria. |
title_short |
Cost-effectiveness of blood agar for isolation of mycobacteria. |
title_full |
Cost-effectiveness of blood agar for isolation of mycobacteria. |
title_fullStr |
Cost-effectiveness of blood agar for isolation of mycobacteria. |
title_full_unstemmed |
Cost-effectiveness of blood agar for isolation of mycobacteria. |
title_sort |
cost-effectiveness of blood agar for isolation of mycobacteria. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2007 |
url |
https://doi.org/10.1371/journal.pntd.0000083 https://doaj.org/article/59d4a39040354321a70b34b381b48dd3 |
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Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 1, Iss 2, p e83 (2007) |
op_relation |
http://europepmc.org/articles/PMC2100370?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0000083 https://doaj.org/article/59d4a39040354321a70b34b381b48dd3 |
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https://doi.org/10.1371/journal.pntd.0000083 |
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PLoS Neglected Tropical Diseases |
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