Morphological and Molecular Description of Sarcocystis myodes n. sp. from the Bank Vole ( Clethrionomys glareolus ) in Lithuania
Numerous rodent species have been broadly examined for Sarcocystis parasites. Nevertheless, recent investigations on Sarcocystis spp. in voles are lacking. As many as 45 bank voles ( Clethrionomys glareolus ) captured in several locations in Lithuania were examined in the present study. Based on mor...
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ftdoajarticles:oai:doaj.org/article:59a6ee2f2f53450199aafd895dc825bc 2023-10-01T03:59:07+02:00 Morphological and Molecular Description of Sarcocystis myodes n. sp. from the Bank Vole ( Clethrionomys glareolus ) in Lithuania Eglė Rudaitytė-Lukošienė Marius Jasiulionis Linas Balčiauskas Petras Prakas Vitalijus Stirkė Dalius Butkauskas 2022-03-01T00:00:00Z https://doi.org/10.3390/biology11040512 https://doaj.org/article/59a6ee2f2f53450199aafd895dc825bc EN eng MDPI AG https://www.mdpi.com/2079-7737/11/4/512 https://doaj.org/toc/2079-7737 doi:10.3390/biology11040512 2079-7737 https://doaj.org/article/59a6ee2f2f53450199aafd895dc825bc Biology, Vol 11, Iss 512, p 512 (2022) bank vole Sarcocystis myodes species description microscopy molecular characterization phylogeny Biology (General) QH301-705.5 article 2022 ftdoajarticles https://doi.org/10.3390/biology11040512 2023-09-03T00:38:27Z Numerous rodent species have been broadly examined for Sarcocystis parasites. Nevertheless, recent investigations on Sarcocystis spp. in voles are lacking. As many as 45 bank voles ( Clethrionomys glareolus ) captured in several locations in Lithuania were examined in the present study. Based on morphological, genetic, and phylogenetic results, sarcocysts detected in one bank vole were described as Sarcocystis myodes n. sp. Using light microscopy analysis, the observed sarcocysts were ribbon-shaped, 6000–3000 × 70–220 µm in size. Sarcocysts were characterized by a relatively thin (about 1 μm) and apparently smooth cyst wall. The lancet-shaped bradyzoites were 9.6–12.0 × 3.1–4.6 μm in size. By transmission electron microscopy, the sarcocyst wall was up to 1 μm thick, parasitophorous vacuolar membrane had small knob-like blebs. Based on 18S rDNA, 28S rDNA, cox1 , rpoB , and ITS1 loci, S . myodes showed highest similarity with S . ratti from the black rat ( Rattus rattus ). According to phylogenetic placement, S . myodes was most closely related to Sarcocystis spp. that employ predatory mammals as their definitive hosts. Morphologically, sarcocysts of S . myodes have similar features to those of S . cernae , S . dirumpens , and S . montanaensis described in voles, however, they use birds of prey or snakes as their definitive hosts. Article in Journal/Newspaper Rattus rattus Directory of Open Access Journals: DOAJ Articles Biology 11 4 512 |
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language |
English |
topic |
bank vole Sarcocystis myodes species description microscopy molecular characterization phylogeny Biology (General) QH301-705.5 |
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bank vole Sarcocystis myodes species description microscopy molecular characterization phylogeny Biology (General) QH301-705.5 Eglė Rudaitytė-Lukošienė Marius Jasiulionis Linas Balčiauskas Petras Prakas Vitalijus Stirkė Dalius Butkauskas Morphological and Molecular Description of Sarcocystis myodes n. sp. from the Bank Vole ( Clethrionomys glareolus ) in Lithuania |
topic_facet |
bank vole Sarcocystis myodes species description microscopy molecular characterization phylogeny Biology (General) QH301-705.5 |
description |
Numerous rodent species have been broadly examined for Sarcocystis parasites. Nevertheless, recent investigations on Sarcocystis spp. in voles are lacking. As many as 45 bank voles ( Clethrionomys glareolus ) captured in several locations in Lithuania were examined in the present study. Based on morphological, genetic, and phylogenetic results, sarcocysts detected in one bank vole were described as Sarcocystis myodes n. sp. Using light microscopy analysis, the observed sarcocysts were ribbon-shaped, 6000–3000 × 70–220 µm in size. Sarcocysts were characterized by a relatively thin (about 1 μm) and apparently smooth cyst wall. The lancet-shaped bradyzoites were 9.6–12.0 × 3.1–4.6 μm in size. By transmission electron microscopy, the sarcocyst wall was up to 1 μm thick, parasitophorous vacuolar membrane had small knob-like blebs. Based on 18S rDNA, 28S rDNA, cox1 , rpoB , and ITS1 loci, S . myodes showed highest similarity with S . ratti from the black rat ( Rattus rattus ). According to phylogenetic placement, S . myodes was most closely related to Sarcocystis spp. that employ predatory mammals as their definitive hosts. Morphologically, sarcocysts of S . myodes have similar features to those of S . cernae , S . dirumpens , and S . montanaensis described in voles, however, they use birds of prey or snakes as their definitive hosts. |
format |
Article in Journal/Newspaper |
author |
Eglė Rudaitytė-Lukošienė Marius Jasiulionis Linas Balčiauskas Petras Prakas Vitalijus Stirkė Dalius Butkauskas |
author_facet |
Eglė Rudaitytė-Lukošienė Marius Jasiulionis Linas Balčiauskas Petras Prakas Vitalijus Stirkė Dalius Butkauskas |
author_sort |
Eglė Rudaitytė-Lukošienė |
title |
Morphological and Molecular Description of Sarcocystis myodes n. sp. from the Bank Vole ( Clethrionomys glareolus ) in Lithuania |
title_short |
Morphological and Molecular Description of Sarcocystis myodes n. sp. from the Bank Vole ( Clethrionomys glareolus ) in Lithuania |
title_full |
Morphological and Molecular Description of Sarcocystis myodes n. sp. from the Bank Vole ( Clethrionomys glareolus ) in Lithuania |
title_fullStr |
Morphological and Molecular Description of Sarcocystis myodes n. sp. from the Bank Vole ( Clethrionomys glareolus ) in Lithuania |
title_full_unstemmed |
Morphological and Molecular Description of Sarcocystis myodes n. sp. from the Bank Vole ( Clethrionomys glareolus ) in Lithuania |
title_sort |
morphological and molecular description of sarcocystis myodes n. sp. from the bank vole ( clethrionomys glareolus ) in lithuania |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/biology11040512 https://doaj.org/article/59a6ee2f2f53450199aafd895dc825bc |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
Biology, Vol 11, Iss 512, p 512 (2022) |
op_relation |
https://www.mdpi.com/2079-7737/11/4/512 https://doaj.org/toc/2079-7737 doi:10.3390/biology11040512 2079-7737 https://doaj.org/article/59a6ee2f2f53450199aafd895dc825bc |
op_doi |
https://doi.org/10.3390/biology11040512 |
container_title |
Biology |
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11 |
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4 |
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512 |
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1778532674568192000 |