Piperlongumine inhibits cell growth and enhances TRAIL-induced apoptosis in prostate cancer cells
Objective: To investigate whether piperlongumine can sensitize prostate cancer cells to tumor necrosis factor-related apoptosis- inducing ligand (TRAIL) and trigger apoptosis in prostate cells. Methods: Human prostate cancer cell lines PC3, LNCaP, and VCaP were cultured with piperlongumine and TRAIL...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2020
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Online Access: | https://doi.org/10.4103/2221-1691.281465 https://doaj.org/article/599d2cc38da94c908bdab4ff66c07b86 |
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ftdoajarticles:oai:doaj.org/article:599d2cc38da94c908bdab4ff66c07b86 2023-05-15T15:05:35+02:00 Piperlongumine inhibits cell growth and enhances TRAIL-induced apoptosis in prostate cancer cells Gorkem Kismali Ahmet Ceylan Ogunc Meral Merve Alpay Funda Kosova Dilek Ulker Cakir Begum Yurdakok-Dikmen Neslihan Tascene Tevhide Sel 2020-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.281465 https://doaj.org/article/599d2cc38da94c908bdab4ff66c07b86 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=5;spage=216;epage=223;aulast=Kismali https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.281465 https://doaj.org/article/599d2cc38da94c908bdab4ff66c07b86 Asian Pacific Journal of Tropical Biomedicine, Vol 10, Iss 5, Pp 216-223 (2020) piperlongumine prostate cancer apoptosis tumor necrosis factor-related apoptosis-inducing ligand Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2020 ftdoajarticles https://doi.org/10.4103/2221-1691.281465 2022-12-31T10:04:36Z Objective: To investigate whether piperlongumine can sensitize prostate cancer cells to tumor necrosis factor-related apoptosis- inducing ligand (TRAIL) and trigger apoptosis in prostate cells. Methods: Human prostate cancer cell lines PC3, LNCaP, and VCaP were cultured with piperlongumine and TRAIL. Then, cell proliferation, migration, caspase activation, apoptotic protein expressions, and death receptor expressions were measured. Results: Piperlongumine inhibited cell proliferation at low doses (<10 μM) alone and in combination with TRAIL (25 ng/mL), induced apoptosis, and suppressed cyclooxygenase activation. Additionally, piperlongumine induced expression of death receptors which potentiated TRAIL-induced apoptosis in cancer cells but did not affect decoy receptors. Piperlongumine also downregulated tumor cell-survival pathways, inhibited colony formation and migration of cancer cells alone or in combination with TRAIL. The combination of piperlongumine with TRAIL was found to be synergistic. Conclusions: Our findings indicate that piperlongumine can sensitize cancer cells to TRAIL through the upregulation of death receptors and can trigger apoptosis with the downregulation of anti- apoptotic proteins. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 10 5 216 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
piperlongumine prostate cancer apoptosis tumor necrosis factor-related apoptosis-inducing ligand Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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piperlongumine prostate cancer apoptosis tumor necrosis factor-related apoptosis-inducing ligand Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Gorkem Kismali Ahmet Ceylan Ogunc Meral Merve Alpay Funda Kosova Dilek Ulker Cakir Begum Yurdakok-Dikmen Neslihan Tascene Tevhide Sel Piperlongumine inhibits cell growth and enhances TRAIL-induced apoptosis in prostate cancer cells |
topic_facet |
piperlongumine prostate cancer apoptosis tumor necrosis factor-related apoptosis-inducing ligand Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate whether piperlongumine can sensitize prostate cancer cells to tumor necrosis factor-related apoptosis- inducing ligand (TRAIL) and trigger apoptosis in prostate cells. Methods: Human prostate cancer cell lines PC3, LNCaP, and VCaP were cultured with piperlongumine and TRAIL. Then, cell proliferation, migration, caspase activation, apoptotic protein expressions, and death receptor expressions were measured. Results: Piperlongumine inhibited cell proliferation at low doses (<10 μM) alone and in combination with TRAIL (25 ng/mL), induced apoptosis, and suppressed cyclooxygenase activation. Additionally, piperlongumine induced expression of death receptors which potentiated TRAIL-induced apoptosis in cancer cells but did not affect decoy receptors. Piperlongumine also downregulated tumor cell-survival pathways, inhibited colony formation and migration of cancer cells alone or in combination with TRAIL. The combination of piperlongumine with TRAIL was found to be synergistic. Conclusions: Our findings indicate that piperlongumine can sensitize cancer cells to TRAIL through the upregulation of death receptors and can trigger apoptosis with the downregulation of anti- apoptotic proteins. |
format |
Article in Journal/Newspaper |
author |
Gorkem Kismali Ahmet Ceylan Ogunc Meral Merve Alpay Funda Kosova Dilek Ulker Cakir Begum Yurdakok-Dikmen Neslihan Tascene Tevhide Sel |
author_facet |
Gorkem Kismali Ahmet Ceylan Ogunc Meral Merve Alpay Funda Kosova Dilek Ulker Cakir Begum Yurdakok-Dikmen Neslihan Tascene Tevhide Sel |
author_sort |
Gorkem Kismali |
title |
Piperlongumine inhibits cell growth and enhances TRAIL-induced apoptosis in prostate cancer cells |
title_short |
Piperlongumine inhibits cell growth and enhances TRAIL-induced apoptosis in prostate cancer cells |
title_full |
Piperlongumine inhibits cell growth and enhances TRAIL-induced apoptosis in prostate cancer cells |
title_fullStr |
Piperlongumine inhibits cell growth and enhances TRAIL-induced apoptosis in prostate cancer cells |
title_full_unstemmed |
Piperlongumine inhibits cell growth and enhances TRAIL-induced apoptosis in prostate cancer cells |
title_sort |
piperlongumine inhibits cell growth and enhances trail-induced apoptosis in prostate cancer cells |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2020 |
url |
https://doi.org/10.4103/2221-1691.281465 https://doaj.org/article/599d2cc38da94c908bdab4ff66c07b86 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 10, Iss 5, Pp 216-223 (2020) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=5;spage=216;epage=223;aulast=Kismali https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.281465 https://doaj.org/article/599d2cc38da94c908bdab4ff66c07b86 |
op_doi |
https://doi.org/10.4103/2221-1691.281465 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
10 |
container_issue |
5 |
container_start_page |
216 |
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1766337260374982656 |