Graphene-Based and Surface-Enhanced Raman Spectroscopy for Monitoring the Physio-Chemical Response of Thermophilic Bacterial Spores to Low Temperatures Exposure

Monitoring the spore life cycle is one of the main issues in several fields including environmental control, sustainable ecosystems, food security, and healthcare systems. In this framework, the study of the living organism resistance to extreme conditions like those mimicking space environments is...

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Published in:Sensors
Main Authors: Carlo Camerlingo, Giuseppe Di Meo, Maria Lepore, Mikhail Lisitskiy, Annarita Poli, Marianna Portaccio, Ida Romano, Paola Di Donato
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2020
Subjects:
Online Access:https://doi.org/10.3390/s20154150
https://doaj.org/article/596c84ff1d894e7195eac7f2a0224132
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spelling ftdoajarticles:oai:doaj.org/article:596c84ff1d894e7195eac7f2a0224132 2023-05-15T13:35:03+02:00 Graphene-Based and Surface-Enhanced Raman Spectroscopy for Monitoring the Physio-Chemical Response of Thermophilic Bacterial Spores to Low Temperatures Exposure Carlo Camerlingo Giuseppe Di Meo Maria Lepore Mikhail Lisitskiy Annarita Poli Marianna Portaccio Ida Romano Paola Di Donato 2020-07-01T00:00:00Z https://doi.org/10.3390/s20154150 https://doaj.org/article/596c84ff1d894e7195eac7f2a0224132 EN eng MDPI AG https://www.mdpi.com/1424-8220/20/15/4150 https://doaj.org/toc/1424-8220 doi:10.3390/s20154150 1424-8220 https://doaj.org/article/596c84ff1d894e7195eac7f2a0224132 Sensors, Vol 20, Iss 4150, p 4150 (2020) thermophilic bacteria spore germination SERS graphene-based pH-meter Chemical technology TP1-1185 article 2020 ftdoajarticles https://doi.org/10.3390/s20154150 2022-12-31T00:16:34Z Monitoring the spore life cycle is one of the main issues in several fields including environmental control, sustainable ecosystems, food security, and healthcare systems. In this framework, the study of the living organism resistance to extreme conditions like those mimicking space environments is particularly interesting. The assessment of the local change of the pH level can be extremely useful for this purpose. An optical physiometer method based on the Raman response of the graphene, which is able to locally sense pH of a fluid on a micrometric scale, has been recently proposed. Due to the presence of <math display="inline"> <semantics> <mi>π</mi> </semantics> </math> -bonds at the surface, the electronic doping of graphene is determined by the external conditions and can be electrochemically controlled or altered by the contact with an acid or alkaline fluid. The doping level affects the vibrational energies of the graphene that can be monitored by conventional Raman spectroscopy. In addition, Surface-Enhanced Raman Spectroscopy (SERS) can give direct information on the biochemical changes occurring in spore components. In this work, we propose the joint use of Graphene-Based Raman Spectroscopy (GbRS) and SERS for the monitoring of the response of spores to exposure to low temperatures down to 100 K. The spores of the thermophilic bacterium Parageobacillus thermantarcticus isolated from an active volcano of Antarctica (Mt. Melbourne) were investigated. These spores are particularly resistant to several stressing stimuli and able to adapt to extreme conditions like low temperatures, UV irradiation, and <math display="inline"> <semantics> <mi>γ</mi> </semantics> </math> -rays exposure. The results obtained showed that the joint use of GbRS and SERS represents a valuable tool for monitoring the physio-chemical response of bacterial spores upon exposure to stressing stimuli. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Sensors 20 15 4150
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic thermophilic bacteria
spore germination
SERS
graphene-based pH-meter
Chemical technology
TP1-1185
spellingShingle thermophilic bacteria
spore germination
SERS
graphene-based pH-meter
Chemical technology
TP1-1185
Carlo Camerlingo
Giuseppe Di Meo
Maria Lepore
Mikhail Lisitskiy
Annarita Poli
Marianna Portaccio
Ida Romano
Paola Di Donato
Graphene-Based and Surface-Enhanced Raman Spectroscopy for Monitoring the Physio-Chemical Response of Thermophilic Bacterial Spores to Low Temperatures Exposure
topic_facet thermophilic bacteria
spore germination
SERS
graphene-based pH-meter
Chemical technology
TP1-1185
description Monitoring the spore life cycle is one of the main issues in several fields including environmental control, sustainable ecosystems, food security, and healthcare systems. In this framework, the study of the living organism resistance to extreme conditions like those mimicking space environments is particularly interesting. The assessment of the local change of the pH level can be extremely useful for this purpose. An optical physiometer method based on the Raman response of the graphene, which is able to locally sense pH of a fluid on a micrometric scale, has been recently proposed. Due to the presence of <math display="inline"> <semantics> <mi>π</mi> </semantics> </math> -bonds at the surface, the electronic doping of graphene is determined by the external conditions and can be electrochemically controlled or altered by the contact with an acid or alkaline fluid. The doping level affects the vibrational energies of the graphene that can be monitored by conventional Raman spectroscopy. In addition, Surface-Enhanced Raman Spectroscopy (SERS) can give direct information on the biochemical changes occurring in spore components. In this work, we propose the joint use of Graphene-Based Raman Spectroscopy (GbRS) and SERS for the monitoring of the response of spores to exposure to low temperatures down to 100 K. The spores of the thermophilic bacterium Parageobacillus thermantarcticus isolated from an active volcano of Antarctica (Mt. Melbourne) were investigated. These spores are particularly resistant to several stressing stimuli and able to adapt to extreme conditions like low temperatures, UV irradiation, and <math display="inline"> <semantics> <mi>γ</mi> </semantics> </math> -rays exposure. The results obtained showed that the joint use of GbRS and SERS represents a valuable tool for monitoring the physio-chemical response of bacterial spores upon exposure to stressing stimuli.
format Article in Journal/Newspaper
author Carlo Camerlingo
Giuseppe Di Meo
Maria Lepore
Mikhail Lisitskiy
Annarita Poli
Marianna Portaccio
Ida Romano
Paola Di Donato
author_facet Carlo Camerlingo
Giuseppe Di Meo
Maria Lepore
Mikhail Lisitskiy
Annarita Poli
Marianna Portaccio
Ida Romano
Paola Di Donato
author_sort Carlo Camerlingo
title Graphene-Based and Surface-Enhanced Raman Spectroscopy for Monitoring the Physio-Chemical Response of Thermophilic Bacterial Spores to Low Temperatures Exposure
title_short Graphene-Based and Surface-Enhanced Raman Spectroscopy for Monitoring the Physio-Chemical Response of Thermophilic Bacterial Spores to Low Temperatures Exposure
title_full Graphene-Based and Surface-Enhanced Raman Spectroscopy for Monitoring the Physio-Chemical Response of Thermophilic Bacterial Spores to Low Temperatures Exposure
title_fullStr Graphene-Based and Surface-Enhanced Raman Spectroscopy for Monitoring the Physio-Chemical Response of Thermophilic Bacterial Spores to Low Temperatures Exposure
title_full_unstemmed Graphene-Based and Surface-Enhanced Raman Spectroscopy for Monitoring the Physio-Chemical Response of Thermophilic Bacterial Spores to Low Temperatures Exposure
title_sort graphene-based and surface-enhanced raman spectroscopy for monitoring the physio-chemical response of thermophilic bacterial spores to low temperatures exposure
publisher MDPI AG
publishDate 2020
url https://doi.org/10.3390/s20154150
https://doaj.org/article/596c84ff1d894e7195eac7f2a0224132
genre Antarc*
Antarctica
genre_facet Antarc*
Antarctica
op_source Sensors, Vol 20, Iss 4150, p 4150 (2020)
op_relation https://www.mdpi.com/1424-8220/20/15/4150
https://doaj.org/toc/1424-8220
doi:10.3390/s20154150
1424-8220
https://doaj.org/article/596c84ff1d894e7195eac7f2a0224132
op_doi https://doi.org/10.3390/s20154150
container_title Sensors
container_volume 20
container_issue 15
container_start_page 4150
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