Técnicas histopatológicas no diagnóstico de criptococose por Cryptococcus deficiente de cápsula: relato de caso Histopathological techniques for diagnosing cryptococcosis due to capsule-deficient Cryptococcus: case report

• Published in: Revista da Sociedade Brasileira de Medicina Tropical
• Main Authors: Alexandra Flávia Gazzoni, Karla Lais Pegas, Luiz Carlos Severo
• Format: Article in Journal/Newspaper
• Language: English
• Published: Sociedade Brasileira de Medicina Tropical (SBMT) 2008
• Subjects: Cryptococcus deficiente de cápsula; Mucicarmim de Mayer; Fontana-Masson; Capsule-deficient Cryptococcus; Mucicarmine; Arctic medicine. Tropical medicine; RC955-962; Arctic; Fontana
• Online Access: https://doi.org/10.1590/S0037-86822008000100015; https://doaj.org/article/591acdaad03e4811b87e059e7fa54b24

Caso de criptococose por Cryptococcus deficiente de cápsula, no qual cultivo do espécime clínico e pesquisa do antígeno capsular no líquor e soro foram negativos. As técnicas histopatológicas foram: Hematoxilina-eosina, Grocott, Mucicarmim de Mayer e Fontana-Masson. Confirmou-se o diagnóstico do Cryptococcus deficiente de cápsula pela técnica de Fontana-Masson e pela imunofluorescência direta. É discutida a potencialidade das técnicas histoquímicas. A case of cryptococcosis due to capsule-deficient Cryptococcus is presented, in which culturing of the clinical specimen and tests for capsular antigen in cerebrospinal fluid and serum were negative. The histopathological techniques evaluated were hematoxylin-eosin, Grocott methenamine silver, Mayer’s mucicarmine and Fontana-Masson. The diagnosis of cryptococcosis due to capsule-deficient Cryptococcus was confirmed by means of the Fontana-Masson technique and by direct immunofluorescence. The potential of the histochemical techniques is discussed.