In silico identification of a candidate synthetic peptide (Tsgf118-43) to monitor human exposure to tsetse flies in West Africa.
BACKGROUND: The analysis of humoral responses directed against the saliva of blood-sucking arthropods was shown to provide epidemiological biomarkers of human exposure to vector-borne diseases. However, the use of whole saliva as antigen presents several limitations such as problems of mass producti...
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ftdoajarticles:oai:doaj.org/article:5913671a8ff740b6845fa5d3f0db3960 2023-05-15T15:11:07+02:00 In silico identification of a candidate synthetic peptide (Tsgf118-43) to monitor human exposure to tsetse flies in West Africa. Emilie Dama Sylvie Cornelie Mamadou Camara Martin Bienvenu Somda Anne Poinsignon Hamidou Ilboudo Emmanuel Elanga Ndille Vincent Jamonneau Philippe Solano Franck Remoue Zakaria Bengaly Adrien Marie Gaston Belem Bruno Bucheton 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002455 https://doaj.org/article/5913671a8ff740b6845fa5d3f0db3960 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3784472?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002455 https://doaj.org/article/5913671a8ff740b6845fa5d3f0db3960 PLoS Neglected Tropical Diseases, Vol 7, Iss 9, p e2455 (2013) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002455 2022-12-31T12:31:34Z BACKGROUND: The analysis of humoral responses directed against the saliva of blood-sucking arthropods was shown to provide epidemiological biomarkers of human exposure to vector-borne diseases. However, the use of whole saliva as antigen presents several limitations such as problems of mass production, reproducibility and specificity. The aim of this study was to design a specific biomarker of exposure to tsetse flies based on the in silico analysis of three Glossina salivary proteins (Ada, Ag5 and Tsgf1) previously shown to be specifically recognized by plasma from exposed individuals. METHODOLOGY/PRINCIPAL FINDINGS: Synthetic peptides were designed by combining several linear epitope prediction methods and Blast analysis. The most specific peptides were then tested by indirect ELISA on a bank of 160 plasma samples from tsetse infested areas and tsetse free areas. Anti-Tsgf118-43 specific IgG levels were low in all three control populations (from rural Africa, urban Africa and Europe) and were significantly higher (p<0.0001) in the two populations exposed to tsetse flies (Guinean HAT foci, and South West Burkina Faso). A positive correlation was also found between Anti-Tsgf118-43 IgG levels and the risk of being infected by Trypanosoma brucei gambiense in the sleeping sickness foci of Guinea. CONCLUSION/SIGNIFICANCE: The Tsgf118-43 peptide is a suitable and promising candidate to develop a standardize immunoassay allowing large scale monitoring of human exposure to tsetse flies in West Africa. This could provide a new surveillance indicator for tsetse control interventions by HAT control programs. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 7 9 e2455 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Emilie Dama Sylvie Cornelie Mamadou Camara Martin Bienvenu Somda Anne Poinsignon Hamidou Ilboudo Emmanuel Elanga Ndille Vincent Jamonneau Philippe Solano Franck Remoue Zakaria Bengaly Adrien Marie Gaston Belem Bruno Bucheton In silico identification of a candidate synthetic peptide (Tsgf118-43) to monitor human exposure to tsetse flies in West Africa. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: The analysis of humoral responses directed against the saliva of blood-sucking arthropods was shown to provide epidemiological biomarkers of human exposure to vector-borne diseases. However, the use of whole saliva as antigen presents several limitations such as problems of mass production, reproducibility and specificity. The aim of this study was to design a specific biomarker of exposure to tsetse flies based on the in silico analysis of three Glossina salivary proteins (Ada, Ag5 and Tsgf1) previously shown to be specifically recognized by plasma from exposed individuals. METHODOLOGY/PRINCIPAL FINDINGS: Synthetic peptides were designed by combining several linear epitope prediction methods and Blast analysis. The most specific peptides were then tested by indirect ELISA on a bank of 160 plasma samples from tsetse infested areas and tsetse free areas. Anti-Tsgf118-43 specific IgG levels were low in all three control populations (from rural Africa, urban Africa and Europe) and were significantly higher (p<0.0001) in the two populations exposed to tsetse flies (Guinean HAT foci, and South West Burkina Faso). A positive correlation was also found between Anti-Tsgf118-43 IgG levels and the risk of being infected by Trypanosoma brucei gambiense in the sleeping sickness foci of Guinea. CONCLUSION/SIGNIFICANCE: The Tsgf118-43 peptide is a suitable and promising candidate to develop a standardize immunoassay allowing large scale monitoring of human exposure to tsetse flies in West Africa. This could provide a new surveillance indicator for tsetse control interventions by HAT control programs. |
format |
Article in Journal/Newspaper |
author |
Emilie Dama Sylvie Cornelie Mamadou Camara Martin Bienvenu Somda Anne Poinsignon Hamidou Ilboudo Emmanuel Elanga Ndille Vincent Jamonneau Philippe Solano Franck Remoue Zakaria Bengaly Adrien Marie Gaston Belem Bruno Bucheton |
author_facet |
Emilie Dama Sylvie Cornelie Mamadou Camara Martin Bienvenu Somda Anne Poinsignon Hamidou Ilboudo Emmanuel Elanga Ndille Vincent Jamonneau Philippe Solano Franck Remoue Zakaria Bengaly Adrien Marie Gaston Belem Bruno Bucheton |
author_sort |
Emilie Dama |
title |
In silico identification of a candidate synthetic peptide (Tsgf118-43) to monitor human exposure to tsetse flies in West Africa. |
title_short |
In silico identification of a candidate synthetic peptide (Tsgf118-43) to monitor human exposure to tsetse flies in West Africa. |
title_full |
In silico identification of a candidate synthetic peptide (Tsgf118-43) to monitor human exposure to tsetse flies in West Africa. |
title_fullStr |
In silico identification of a candidate synthetic peptide (Tsgf118-43) to monitor human exposure to tsetse flies in West Africa. |
title_full_unstemmed |
In silico identification of a candidate synthetic peptide (Tsgf118-43) to monitor human exposure to tsetse flies in West Africa. |
title_sort |
in silico identification of a candidate synthetic peptide (tsgf118-43) to monitor human exposure to tsetse flies in west africa. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pntd.0002455 https://doaj.org/article/5913671a8ff740b6845fa5d3f0db3960 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 7, Iss 9, p e2455 (2013) |
op_relation |
http://europepmc.org/articles/PMC3784472?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002455 https://doaj.org/article/5913671a8ff740b6845fa5d3f0db3960 |
op_doi |
https://doi.org/10.1371/journal.pntd.0002455 |
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PLoS Neglected Tropical Diseases |
container_volume |
7 |
container_issue |
9 |
container_start_page |
e2455 |
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1766342018604204032 |