Infectious salmon anaemia virus infection of Atlantic salmon gill epithelial cells
Abstract Infectious salmon anaemia virus (ISAV), a member of the Orthomyxoviridae family, infects and causes disease in farmed Atlantic salmon ( Salmo salar L.). Previous studies have shown Atlantic salmon endothelial cells to be the primary targets of ISAV infection. However, it is not known if cel...
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ftdoajarticles:oai:doaj.org/article:58cf20386f8f440f977094b01e8685b5 2023-05-15T15:28:08+02:00 Infectious salmon anaemia virus infection of Atlantic salmon gill epithelial cells Weli Simon Aamelfot Maria Dale Ole Koppang Erling Falk Knut 2013-01-01T00:00:00Z https://doi.org/10.1186/1743-422X-10-5 https://doaj.org/article/58cf20386f8f440f977094b01e8685b5 EN eng BMC http://www.virologyj.com/content/10/1/5 https://doaj.org/toc/1743-422X doi:10.1186/1743-422X-10-5 1743-422X https://doaj.org/article/58cf20386f8f440f977094b01e8685b5 Virology Journal, Vol 10, Iss 1, p 5 (2013) ISAV Cell tropism Sialic acid receptor Gill epithelial cells Infectious and parasitic diseases RC109-216 article 2013 ftdoajarticles https://doi.org/10.1186/1743-422X-10-5 2022-12-31T00:42:20Z Abstract Infectious salmon anaemia virus (ISAV), a member of the Orthomyxoviridae family, infects and causes disease in farmed Atlantic salmon ( Salmo salar L.). Previous studies have shown Atlantic salmon endothelial cells to be the primary targets of ISAV infection. However, it is not known if cells other than endothelial cells play a role in ISAV tropism. To further assess cell tropism, we examined ISAV infection of Atlantic salmon gill epithelial cells in vivo and in vitro . We demonstrated the susceptibility of epithelial cells to ISAV infection. On comparison of primary gill epithelial cell cultures with ISAV permissive fish cell cultures, we found the virus yield in primary gill epithelial cells to be comparable with that of salmon head kidney (SHK)-1 cells, but lower than TO or Atlantic salmon kidney (ASK)-II cells. Light and transmission electron microscopy (TEM) revealed that the primary gill cells possessed characteristics consistent with epithelial cells. Virus histochemistry showed that gill epithelial cells expressed 4- O -acetylated sialic acid which is recognized as the ISAV receptor. To the best of our knowledge, this is the first demonstration of ISAV infection in Atlantic salmon primary gill epithelial cells. This study thus broadens our understanding of cell tropism and transmission of ISAV in Atlantic salmon. Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles Virology Journal 10 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
ISAV Cell tropism Sialic acid receptor Gill epithelial cells Infectious and parasitic diseases RC109-216 |
spellingShingle |
ISAV Cell tropism Sialic acid receptor Gill epithelial cells Infectious and parasitic diseases RC109-216 Weli Simon Aamelfot Maria Dale Ole Koppang Erling Falk Knut Infectious salmon anaemia virus infection of Atlantic salmon gill epithelial cells |
topic_facet |
ISAV Cell tropism Sialic acid receptor Gill epithelial cells Infectious and parasitic diseases RC109-216 |
description |
Abstract Infectious salmon anaemia virus (ISAV), a member of the Orthomyxoviridae family, infects and causes disease in farmed Atlantic salmon ( Salmo salar L.). Previous studies have shown Atlantic salmon endothelial cells to be the primary targets of ISAV infection. However, it is not known if cells other than endothelial cells play a role in ISAV tropism. To further assess cell tropism, we examined ISAV infection of Atlantic salmon gill epithelial cells in vivo and in vitro . We demonstrated the susceptibility of epithelial cells to ISAV infection. On comparison of primary gill epithelial cell cultures with ISAV permissive fish cell cultures, we found the virus yield in primary gill epithelial cells to be comparable with that of salmon head kidney (SHK)-1 cells, but lower than TO or Atlantic salmon kidney (ASK)-II cells. Light and transmission electron microscopy (TEM) revealed that the primary gill cells possessed characteristics consistent with epithelial cells. Virus histochemistry showed that gill epithelial cells expressed 4- O -acetylated sialic acid which is recognized as the ISAV receptor. To the best of our knowledge, this is the first demonstration of ISAV infection in Atlantic salmon primary gill epithelial cells. This study thus broadens our understanding of cell tropism and transmission of ISAV in Atlantic salmon. |
format |
Article in Journal/Newspaper |
author |
Weli Simon Aamelfot Maria Dale Ole Koppang Erling Falk Knut |
author_facet |
Weli Simon Aamelfot Maria Dale Ole Koppang Erling Falk Knut |
author_sort |
Weli Simon |
title |
Infectious salmon anaemia virus infection of Atlantic salmon gill epithelial cells |
title_short |
Infectious salmon anaemia virus infection of Atlantic salmon gill epithelial cells |
title_full |
Infectious salmon anaemia virus infection of Atlantic salmon gill epithelial cells |
title_fullStr |
Infectious salmon anaemia virus infection of Atlantic salmon gill epithelial cells |
title_full_unstemmed |
Infectious salmon anaemia virus infection of Atlantic salmon gill epithelial cells |
title_sort |
infectious salmon anaemia virus infection of atlantic salmon gill epithelial cells |
publisher |
BMC |
publishDate |
2013 |
url |
https://doi.org/10.1186/1743-422X-10-5 https://doaj.org/article/58cf20386f8f440f977094b01e8685b5 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Virology Journal, Vol 10, Iss 1, p 5 (2013) |
op_relation |
http://www.virologyj.com/content/10/1/5 https://doaj.org/toc/1743-422X doi:10.1186/1743-422X-10-5 1743-422X https://doaj.org/article/58cf20386f8f440f977094b01e8685b5 |
op_doi |
https://doi.org/10.1186/1743-422X-10-5 |
container_title |
Virology Journal |
container_volume |
10 |
container_issue |
1 |
_version_ |
1766358524813639680 |