Zika virus inhibits eIF2α-dependent stress granule assembly.
Zika virus (ZIKV), a member of the Flaviviridae family, is the most recent emerging arbovirus with pandemic potential. During infection, viruses trigger the host cell stress response, leading to changes in RNA translation and the assembly of large aggregates of stalled translation preinitiation comp...
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ftdoajarticles:oai:doaj.org/article:58cc52a51a9a490abec564537506f0b5 2023-05-15T15:06:57+02:00 Zika virus inhibits eIF2α-dependent stress granule assembly. Raquel Amorim Abdelkrim Temzi Bryan D Griffin Andrew J Mouland 2017-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005775 https://doaj.org/article/58cc52a51a9a490abec564537506f0b5 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5531678?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005775 https://doaj.org/article/58cc52a51a9a490abec564537506f0b5 PLoS Neglected Tropical Diseases, Vol 11, Iss 7, p e0005775 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005775 2022-12-31T02:16:11Z Zika virus (ZIKV), a member of the Flaviviridae family, is the most recent emerging arbovirus with pandemic potential. During infection, viruses trigger the host cell stress response, leading to changes in RNA translation and the assembly of large aggregates of stalled translation preinitiation complexes, termed stress granules (SGs). Several reports demonstrate that flaviviruses modulate the assembly of stress granules (SG). As an emerging pathogen, little is known however about how ZIKV modulates the host cell stress response. In this work, we investigate how ZIKV modulates SG assembly. We demonstrate that ZIKV negatively impacts SG assembly under oxidative stress conditions induced by sodium arsenite (Ars), a treatment that leads to the phosphorylation of eIF2α. By contrast, no measurable difference in SG assembly was observed between mock and ZIKV-infected cells treated with sodium selenite (Se) or Pateamine A (PatA), compounds that trigger eIF2α-independent SG assembly. Interestingly, ZIKV infection markedly impaired the phosphorylation of eIF2α triggered in Ars-treated infected cells, and the abrogation of SG assembly in ZIKV-infected cells is, at least in part, dependent on eIF2α dephosphorylation. These data demonstrate that ZIKV elicits mechanisms to counteract host anti-viral stress responses to promote a cellular environment propitious for viral replication. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 7 e0005775 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Raquel Amorim Abdelkrim Temzi Bryan D Griffin Andrew J Mouland Zika virus inhibits eIF2α-dependent stress granule assembly. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Zika virus (ZIKV), a member of the Flaviviridae family, is the most recent emerging arbovirus with pandemic potential. During infection, viruses trigger the host cell stress response, leading to changes in RNA translation and the assembly of large aggregates of stalled translation preinitiation complexes, termed stress granules (SGs). Several reports demonstrate that flaviviruses modulate the assembly of stress granules (SG). As an emerging pathogen, little is known however about how ZIKV modulates the host cell stress response. In this work, we investigate how ZIKV modulates SG assembly. We demonstrate that ZIKV negatively impacts SG assembly under oxidative stress conditions induced by sodium arsenite (Ars), a treatment that leads to the phosphorylation of eIF2α. By contrast, no measurable difference in SG assembly was observed between mock and ZIKV-infected cells treated with sodium selenite (Se) or Pateamine A (PatA), compounds that trigger eIF2α-independent SG assembly. Interestingly, ZIKV infection markedly impaired the phosphorylation of eIF2α triggered in Ars-treated infected cells, and the abrogation of SG assembly in ZIKV-infected cells is, at least in part, dependent on eIF2α dephosphorylation. These data demonstrate that ZIKV elicits mechanisms to counteract host anti-viral stress responses to promote a cellular environment propitious for viral replication. |
format |
Article in Journal/Newspaper |
author |
Raquel Amorim Abdelkrim Temzi Bryan D Griffin Andrew J Mouland |
author_facet |
Raquel Amorim Abdelkrim Temzi Bryan D Griffin Andrew J Mouland |
author_sort |
Raquel Amorim |
title |
Zika virus inhibits eIF2α-dependent stress granule assembly. |
title_short |
Zika virus inhibits eIF2α-dependent stress granule assembly. |
title_full |
Zika virus inhibits eIF2α-dependent stress granule assembly. |
title_fullStr |
Zika virus inhibits eIF2α-dependent stress granule assembly. |
title_full_unstemmed |
Zika virus inhibits eIF2α-dependent stress granule assembly. |
title_sort |
zika virus inhibits eif2α-dependent stress granule assembly. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005775 https://doaj.org/article/58cc52a51a9a490abec564537506f0b5 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 7, p e0005775 (2017) |
op_relation |
http://europepmc.org/articles/PMC5531678?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005775 https://doaj.org/article/58cc52a51a9a490abec564537506f0b5 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005775 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
7 |
container_start_page |
e0005775 |
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1766338532953030656 |