Assessment of platelet indices and platelet activation markers in children with Plasmodium falciparum malaria
Abstract Background Plasmodium falciparum malaria remains one of the world’s major infectious diseases that cause most morbidity and mortality, particularly in children. In Ghana, most children below the ages of 5 years depending on the severity of the infection often lose their lives. However, it i...
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ftdoajarticles:oai:doaj.org/article:58b2183373ab4143ad4a49180a5ea5a8 2023-05-15T15:15:43+02:00 Assessment of platelet indices and platelet activation markers in children with Plasmodium falciparum malaria Renate Asare Clement Opoku-Okrah Kwabena Owusu Danquah Ohene Opare-Sem Otchere Addai-Mensah Daniel Gyamfi Francis Agyei Amponsah Edward Y. Afriyie Richard Vikpebah Duneeh David Ntiamoah Ofosu Michael Frimpong 2020-04-01T00:00:00Z https://doi.org/10.1186/s12936-020-03218-4 https://doaj.org/article/58b2183373ab4143ad4a49180a5ea5a8 EN eng BMC http://link.springer.com/article/10.1186/s12936-020-03218-4 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03218-4 1475-2875 https://doaj.org/article/58b2183373ab4143ad4a49180a5ea5a8 Malaria Journal, Vol 19, Iss 1, Pp 1-7 (2020) Platelet membrane glycoproteins Platelet indices Plasmodium falciparum Flow cytometry Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2020 ftdoajarticles https://doi.org/10.1186/s12936-020-03218-4 2022-12-31T00:08:03Z Abstract Background Plasmodium falciparum malaria remains one of the world’s major infectious diseases that cause most morbidity and mortality, particularly in children. In Ghana, most children below the ages of 5 years depending on the severity of the infection often lose their lives. However, it is still debatable why infection with falciparum malaria contributes to thrombocytopenia. Methods This study sought to investigate the expression of the various platelet indices and activation markers in children with falciparum malaria. Platelet indices (Platelet count [PLT], Plateletcrite [PCT], Mean Platelet Volume [MPV], Platelet Distribution Width [PDW] and Platelet-Large Cell Ratio [P-LCR]) and platelet surface membrane glycoproteins (GPIIb/IIIa [PAC-1], P-selectin [CD62p] and GPIV [CD36]) expressions were determined in children with falciparum malaria (cases) and healthy children (controls) using automated blood cell analysis and flow cytometry techniques, respectively. Results Except for P-LCR, all the other platelet indices (PLT, MPV, PDW, and PCT) were significantly lower in the cases than the controls (P < 0.05). Also, it was observed that the level of expression of the activation markers; PAC 1 and CD62p showed a significant (P < 0.05) decreased before and after activation in falciparum malaria cases than in the controls. On the contrary, CD36 expression in the controls did not differ significantly (p > 0.05) from the malaria cases. Platelet activation markers were known to be associated with increased risk of falciparum malaria with the mean fluorescence intensity of PAC1 (Odds Ratio [OR] 34.0, Relative Risk [RR] 4.47, 95% Confidence Interval [CI] 4.904–235.7; p < 0.0001) and CD36 (OR 4.2, RR 1.82, 95% CI 0.9824–17.96; p = 0.04). Moreover, the percentage expression of CD62p (OR 4.0, RR 1.80, 95% CI 0.59–27.24; p = 0.19) was also observed to be probably associated with increased risk of falciparum malaria although not statistically significant (p > 0.05). Conclusion Plasmodium falciparum ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 19 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Platelet membrane glycoproteins Platelet indices Plasmodium falciparum Flow cytometry Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Platelet membrane glycoproteins Platelet indices Plasmodium falciparum Flow cytometry Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Renate Asare Clement Opoku-Okrah Kwabena Owusu Danquah Ohene Opare-Sem Otchere Addai-Mensah Daniel Gyamfi Francis Agyei Amponsah Edward Y. Afriyie Richard Vikpebah Duneeh David Ntiamoah Ofosu Michael Frimpong Assessment of platelet indices and platelet activation markers in children with Plasmodium falciparum malaria |
topic_facet |
Platelet membrane glycoproteins Platelet indices Plasmodium falciparum Flow cytometry Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Plasmodium falciparum malaria remains one of the world’s major infectious diseases that cause most morbidity and mortality, particularly in children. In Ghana, most children below the ages of 5 years depending on the severity of the infection often lose their lives. However, it is still debatable why infection with falciparum malaria contributes to thrombocytopenia. Methods This study sought to investigate the expression of the various platelet indices and activation markers in children with falciparum malaria. Platelet indices (Platelet count [PLT], Plateletcrite [PCT], Mean Platelet Volume [MPV], Platelet Distribution Width [PDW] and Platelet-Large Cell Ratio [P-LCR]) and platelet surface membrane glycoproteins (GPIIb/IIIa [PAC-1], P-selectin [CD62p] and GPIV [CD36]) expressions were determined in children with falciparum malaria (cases) and healthy children (controls) using automated blood cell analysis and flow cytometry techniques, respectively. Results Except for P-LCR, all the other platelet indices (PLT, MPV, PDW, and PCT) were significantly lower in the cases than the controls (P < 0.05). Also, it was observed that the level of expression of the activation markers; PAC 1 and CD62p showed a significant (P < 0.05) decreased before and after activation in falciparum malaria cases than in the controls. On the contrary, CD36 expression in the controls did not differ significantly (p > 0.05) from the malaria cases. Platelet activation markers were known to be associated with increased risk of falciparum malaria with the mean fluorescence intensity of PAC1 (Odds Ratio [OR] 34.0, Relative Risk [RR] 4.47, 95% Confidence Interval [CI] 4.904–235.7; p < 0.0001) and CD36 (OR 4.2, RR 1.82, 95% CI 0.9824–17.96; p = 0.04). Moreover, the percentage expression of CD62p (OR 4.0, RR 1.80, 95% CI 0.59–27.24; p = 0.19) was also observed to be probably associated with increased risk of falciparum malaria although not statistically significant (p > 0.05). Conclusion Plasmodium falciparum ... |
format |
Article in Journal/Newspaper |
author |
Renate Asare Clement Opoku-Okrah Kwabena Owusu Danquah Ohene Opare-Sem Otchere Addai-Mensah Daniel Gyamfi Francis Agyei Amponsah Edward Y. Afriyie Richard Vikpebah Duneeh David Ntiamoah Ofosu Michael Frimpong |
author_facet |
Renate Asare Clement Opoku-Okrah Kwabena Owusu Danquah Ohene Opare-Sem Otchere Addai-Mensah Daniel Gyamfi Francis Agyei Amponsah Edward Y. Afriyie Richard Vikpebah Duneeh David Ntiamoah Ofosu Michael Frimpong |
author_sort |
Renate Asare |
title |
Assessment of platelet indices and platelet activation markers in children with Plasmodium falciparum malaria |
title_short |
Assessment of platelet indices and platelet activation markers in children with Plasmodium falciparum malaria |
title_full |
Assessment of platelet indices and platelet activation markers in children with Plasmodium falciparum malaria |
title_fullStr |
Assessment of platelet indices and platelet activation markers in children with Plasmodium falciparum malaria |
title_full_unstemmed |
Assessment of platelet indices and platelet activation markers in children with Plasmodium falciparum malaria |
title_sort |
assessment of platelet indices and platelet activation markers in children with plasmodium falciparum malaria |
publisher |
BMC |
publishDate |
2020 |
url |
https://doi.org/10.1186/s12936-020-03218-4 https://doaj.org/article/58b2183373ab4143ad4a49180a5ea5a8 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 19, Iss 1, Pp 1-7 (2020) |
op_relation |
http://link.springer.com/article/10.1186/s12936-020-03218-4 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03218-4 1475-2875 https://doaj.org/article/58b2183373ab4143ad4a49180a5ea5a8 |
op_doi |
https://doi.org/10.1186/s12936-020-03218-4 |
container_title |
Malaria Journal |
container_volume |
19 |
container_issue |
1 |
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1766346061213859840 |