Detection and expression of superantigens and resistance in isolates from women carriers of Staphylococcus aureus who take care of and feed children
Introduction: Staphylococcus aureus colonizes mucous membranes and skin causing severe infections in humans and animals. It is important to determine carrier status of enterotoxigenic strains of this microorganism in food handlers to prevent food poisoning. Objective: To establish the correlations a...
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Instituto Nacional de Salud
2018
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ftdoajarticles:oai:doaj.org/article:5869945ea2f5417fb641f7ec7aece704 2023-05-15T15:14:51+02:00 Detection and expression of superantigens and resistance in isolates from women carriers of Staphylococcus aureus who take care of and feed children Yina Marcela Guaca-González Gladys Fernanda Flórez-Restrepo José Ignacio Moncayo-Ortíz Jorge Santacruz-Ibarra Adalucy Álvarez-Aldana 2018-03-01T00:00:00Z https://doi.org/10.7705/biomedica.v38i0.3653 https://doaj.org/article/5869945ea2f5417fb641f7ec7aece704 EN ES eng spa Instituto Nacional de Salud https://www.revistabiomedica.org/index.php/biomedica/article/view/3653 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v38i0.3653 https://doaj.org/article/5869945ea2f5417fb641f7ec7aece704 Biomédica: revista del Instituto Nacional de Salud, Vol 38, Iss 1, Pp 96-104 (2018) Staphylococcus aureus enterotoxinas farmacorresistencia microbiana superantígenos genotipo variación genética Medicine R Arctic medicine. Tropical medicine RC955-962 article 2018 ftdoajarticles https://doi.org/10.7705/biomedica.v38i0.3653 2022-12-30T23:59:18Z Introduction: Staphylococcus aureus colonizes mucous membranes and skin causing severe infections in humans and animals. It is important to determine carrier status of enterotoxigenic strains of this microorganism in food handlers to prevent food poisoning. Objective: To establish the correlations among classic enterotoxigenic genes, tsst-1 gene, the production of toxins in cultures and antimicrobial resistance in S. aureus isolates from women who handle the food, feed and take care of children in their communities. Materials and methods: Nasal swab and finger samples were cultured and S. aureus was identified using routine methods and automated systems. DNA extraction was done by the CTAB modified method, and superantigen detection by simple and multiplex PCR, while toxins were detected using commercial kits. Results: We found that 22.0% of subjects were S. aureus carriers: 17.0% corresponded to nose samples, 5.0% to hands and 6.7% to both nose and hands. The prevalence of superantigens was 73.7%. The most frequent genotype was sea-tsst-1 with 10%. Resistance to one antibiotic was 74.7%, and to four antibiotics, 3.2%; 93.7% of the isolates were betalactamase-positive. Classical genes and tsst-1 gene were detected by PCR in 48.4% of samples and toxins in supernatant were detected in 42.1% of them with 95.7% of correlation.The highest correlations were established for TSST-1 and SEA with 100% and 94.4%, respectively. The correlation of tsst-1 gene with toxin production and resistance was 100%. All isolates with genotype sea-tsst-1 were toxin-positive and resistant. Conclusion: The rate of toxigenic and resistant S. aureus isolates from women in charge of feeding and taking care of children was higher than 70%, which demonstrates its high virulence. This requires the strict application of hygienic and sanitary regulations in order to avoid the risk of food poisoning. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Handle The ENVELOPE(161.983,161.983,-78.000,-78.000) Biomédica 38 1 96 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Spanish |
topic |
Staphylococcus aureus enterotoxinas farmacorresistencia microbiana superantígenos genotipo variación genética Medicine R Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Staphylococcus aureus enterotoxinas farmacorresistencia microbiana superantígenos genotipo variación genética Medicine R Arctic medicine. Tropical medicine RC955-962 Yina Marcela Guaca-González Gladys Fernanda Flórez-Restrepo José Ignacio Moncayo-Ortíz Jorge Santacruz-Ibarra Adalucy Álvarez-Aldana Detection and expression of superantigens and resistance in isolates from women carriers of Staphylococcus aureus who take care of and feed children |
topic_facet |
Staphylococcus aureus enterotoxinas farmacorresistencia microbiana superantígenos genotipo variación genética Medicine R Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction: Staphylococcus aureus colonizes mucous membranes and skin causing severe infections in humans and animals. It is important to determine carrier status of enterotoxigenic strains of this microorganism in food handlers to prevent food poisoning. Objective: To establish the correlations among classic enterotoxigenic genes, tsst-1 gene, the production of toxins in cultures and antimicrobial resistance in S. aureus isolates from women who handle the food, feed and take care of children in their communities. Materials and methods: Nasal swab and finger samples were cultured and S. aureus was identified using routine methods and automated systems. DNA extraction was done by the CTAB modified method, and superantigen detection by simple and multiplex PCR, while toxins were detected using commercial kits. Results: We found that 22.0% of subjects were S. aureus carriers: 17.0% corresponded to nose samples, 5.0% to hands and 6.7% to both nose and hands. The prevalence of superantigens was 73.7%. The most frequent genotype was sea-tsst-1 with 10%. Resistance to one antibiotic was 74.7%, and to four antibiotics, 3.2%; 93.7% of the isolates were betalactamase-positive. Classical genes and tsst-1 gene were detected by PCR in 48.4% of samples and toxins in supernatant were detected in 42.1% of them with 95.7% of correlation.The highest correlations were established for TSST-1 and SEA with 100% and 94.4%, respectively. The correlation of tsst-1 gene with toxin production and resistance was 100%. All isolates with genotype sea-tsst-1 were toxin-positive and resistant. Conclusion: The rate of toxigenic and resistant S. aureus isolates from women in charge of feeding and taking care of children was higher than 70%, which demonstrates its high virulence. This requires the strict application of hygienic and sanitary regulations in order to avoid the risk of food poisoning. |
format |
Article in Journal/Newspaper |
author |
Yina Marcela Guaca-González Gladys Fernanda Flórez-Restrepo José Ignacio Moncayo-Ortíz Jorge Santacruz-Ibarra Adalucy Álvarez-Aldana |
author_facet |
Yina Marcela Guaca-González Gladys Fernanda Flórez-Restrepo José Ignacio Moncayo-Ortíz Jorge Santacruz-Ibarra Adalucy Álvarez-Aldana |
author_sort |
Yina Marcela Guaca-González |
title |
Detection and expression of superantigens and resistance in isolates from women carriers of Staphylococcus aureus who take care of and feed children |
title_short |
Detection and expression of superantigens and resistance in isolates from women carriers of Staphylococcus aureus who take care of and feed children |
title_full |
Detection and expression of superantigens and resistance in isolates from women carriers of Staphylococcus aureus who take care of and feed children |
title_fullStr |
Detection and expression of superantigens and resistance in isolates from women carriers of Staphylococcus aureus who take care of and feed children |
title_full_unstemmed |
Detection and expression of superantigens and resistance in isolates from women carriers of Staphylococcus aureus who take care of and feed children |
title_sort |
detection and expression of superantigens and resistance in isolates from women carriers of staphylococcus aureus who take care of and feed children |
publisher |
Instituto Nacional de Salud |
publishDate |
2018 |
url |
https://doi.org/10.7705/biomedica.v38i0.3653 https://doaj.org/article/5869945ea2f5417fb641f7ec7aece704 |
long_lat |
ENVELOPE(161.983,161.983,-78.000,-78.000) |
geographic |
Arctic Handle The |
geographic_facet |
Arctic Handle The |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Biomédica: revista del Instituto Nacional de Salud, Vol 38, Iss 1, Pp 96-104 (2018) |
op_relation |
https://www.revistabiomedica.org/index.php/biomedica/article/view/3653 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v38i0.3653 https://doaj.org/article/5869945ea2f5417fb641f7ec7aece704 |
op_doi |
https://doi.org/10.7705/biomedica.v38i0.3653 |
container_title |
Biomédica |
container_volume |
38 |
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1 |
container_start_page |
96 |
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