Detection and expression of superantigens and resistance in isolates from women carriers of Staphylococcus aureus who take care of and feed children

Introduction: Staphylococcus aureus colonizes mucous membranes and skin causing severe infections in humans and animals. It is important to determine carrier status of enterotoxigenic strains of this microorganism in food handlers to prevent food poisoning. Objective: To establish the correlations a...

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Published in:Biomédica
Main Authors: Yina Marcela Guaca-González, Gladys Fernanda Flórez-Restrepo, José Ignacio Moncayo-Ortíz, Jorge Santacruz-Ibarra, Adalucy Álvarez-Aldana
Format: Article in Journal/Newspaper
Language:English
Spanish
Published: Instituto Nacional de Salud 2018
Subjects:
R
Online Access:https://doi.org/10.7705/biomedica.v38i0.3653
https://doaj.org/article/5869945ea2f5417fb641f7ec7aece704
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spelling ftdoajarticles:oai:doaj.org/article:5869945ea2f5417fb641f7ec7aece704 2023-05-15T15:14:51+02:00 Detection and expression of superantigens and resistance in isolates from women carriers of Staphylococcus aureus who take care of and feed children Yina Marcela Guaca-González Gladys Fernanda Flórez-Restrepo José Ignacio Moncayo-Ortíz Jorge Santacruz-Ibarra Adalucy Álvarez-Aldana 2018-03-01T00:00:00Z https://doi.org/10.7705/biomedica.v38i0.3653 https://doaj.org/article/5869945ea2f5417fb641f7ec7aece704 EN ES eng spa Instituto Nacional de Salud https://www.revistabiomedica.org/index.php/biomedica/article/view/3653 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v38i0.3653 https://doaj.org/article/5869945ea2f5417fb641f7ec7aece704 Biomédica: revista del Instituto Nacional de Salud, Vol 38, Iss 1, Pp 96-104 (2018) Staphylococcus aureus enterotoxinas farmacorresistencia microbiana superantígenos genotipo variación genética Medicine R Arctic medicine. Tropical medicine RC955-962 article 2018 ftdoajarticles https://doi.org/10.7705/biomedica.v38i0.3653 2022-12-30T23:59:18Z Introduction: Staphylococcus aureus colonizes mucous membranes and skin causing severe infections in humans and animals. It is important to determine carrier status of enterotoxigenic strains of this microorganism in food handlers to prevent food poisoning. Objective: To establish the correlations among classic enterotoxigenic genes, tsst-1 gene, the production of toxins in cultures and antimicrobial resistance in S. aureus isolates from women who handle the food, feed and take care of children in their communities. Materials and methods: Nasal swab and finger samples were cultured and S. aureus was identified using routine methods and automated systems. DNA extraction was done by the CTAB modified method, and superantigen detection by simple and multiplex PCR, while toxins were detected using commercial kits. Results: We found that 22.0% of subjects were S. aureus carriers: 17.0% corresponded to nose samples, 5.0% to hands and 6.7% to both nose and hands. The prevalence of superantigens was 73.7%. The most frequent genotype was sea-tsst-1 with 10%. Resistance to one antibiotic was 74.7%, and to four antibiotics, 3.2%; 93.7% of the isolates were betalactamase-positive. Classical genes and tsst-1 gene were detected by PCR in 48.4% of samples and toxins in supernatant were detected in 42.1% of them with 95.7% of correlation.The highest correlations were established for TSST-1 and SEA with 100% and 94.4%, respectively. The correlation of tsst-1 gene with toxin production and resistance was 100%. All isolates with genotype sea-tsst-1 were toxin-positive and resistant. Conclusion: The rate of toxigenic and resistant S. aureus isolates from women in charge of feeding and taking care of children was higher than 70%, which demonstrates its high virulence. This requires the strict application of hygienic and sanitary regulations in order to avoid the risk of food poisoning. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Handle The ENVELOPE(161.983,161.983,-78.000,-78.000) Biomédica 38 1 96
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
Spanish
topic Staphylococcus aureus
enterotoxinas
farmacorresistencia microbiana
superantígenos
genotipo
variación genética
Medicine
R
Arctic medicine. Tropical medicine
RC955-962
spellingShingle Staphylococcus aureus
enterotoxinas
farmacorresistencia microbiana
superantígenos
genotipo
variación genética
Medicine
R
Arctic medicine. Tropical medicine
RC955-962
Yina Marcela Guaca-González
Gladys Fernanda Flórez-Restrepo
José Ignacio Moncayo-Ortíz
Jorge Santacruz-Ibarra
Adalucy Álvarez-Aldana
Detection and expression of superantigens and resistance in isolates from women carriers of Staphylococcus aureus who take care of and feed children
topic_facet Staphylococcus aureus
enterotoxinas
farmacorresistencia microbiana
superantígenos
genotipo
variación genética
Medicine
R
Arctic medicine. Tropical medicine
RC955-962
description Introduction: Staphylococcus aureus colonizes mucous membranes and skin causing severe infections in humans and animals. It is important to determine carrier status of enterotoxigenic strains of this microorganism in food handlers to prevent food poisoning. Objective: To establish the correlations among classic enterotoxigenic genes, tsst-1 gene, the production of toxins in cultures and antimicrobial resistance in S. aureus isolates from women who handle the food, feed and take care of children in their communities. Materials and methods: Nasal swab and finger samples were cultured and S. aureus was identified using routine methods and automated systems. DNA extraction was done by the CTAB modified method, and superantigen detection by simple and multiplex PCR, while toxins were detected using commercial kits. Results: We found that 22.0% of subjects were S. aureus carriers: 17.0% corresponded to nose samples, 5.0% to hands and 6.7% to both nose and hands. The prevalence of superantigens was 73.7%. The most frequent genotype was sea-tsst-1 with 10%. Resistance to one antibiotic was 74.7%, and to four antibiotics, 3.2%; 93.7% of the isolates were betalactamase-positive. Classical genes and tsst-1 gene were detected by PCR in 48.4% of samples and toxins in supernatant were detected in 42.1% of them with 95.7% of correlation.The highest correlations were established for TSST-1 and SEA with 100% and 94.4%, respectively. The correlation of tsst-1 gene with toxin production and resistance was 100%. All isolates with genotype sea-tsst-1 were toxin-positive and resistant. Conclusion: The rate of toxigenic and resistant S. aureus isolates from women in charge of feeding and taking care of children was higher than 70%, which demonstrates its high virulence. This requires the strict application of hygienic and sanitary regulations in order to avoid the risk of food poisoning.
format Article in Journal/Newspaper
author Yina Marcela Guaca-González
Gladys Fernanda Flórez-Restrepo
José Ignacio Moncayo-Ortíz
Jorge Santacruz-Ibarra
Adalucy Álvarez-Aldana
author_facet Yina Marcela Guaca-González
Gladys Fernanda Flórez-Restrepo
José Ignacio Moncayo-Ortíz
Jorge Santacruz-Ibarra
Adalucy Álvarez-Aldana
author_sort Yina Marcela Guaca-González
title Detection and expression of superantigens and resistance in isolates from women carriers of Staphylococcus aureus who take care of and feed children
title_short Detection and expression of superantigens and resistance in isolates from women carriers of Staphylococcus aureus who take care of and feed children
title_full Detection and expression of superantigens and resistance in isolates from women carriers of Staphylococcus aureus who take care of and feed children
title_fullStr Detection and expression of superantigens and resistance in isolates from women carriers of Staphylococcus aureus who take care of and feed children
title_full_unstemmed Detection and expression of superantigens and resistance in isolates from women carriers of Staphylococcus aureus who take care of and feed children
title_sort detection and expression of superantigens and resistance in isolates from women carriers of staphylococcus aureus who take care of and feed children
publisher Instituto Nacional de Salud
publishDate 2018
url https://doi.org/10.7705/biomedica.v38i0.3653
https://doaj.org/article/5869945ea2f5417fb641f7ec7aece704
long_lat ENVELOPE(161.983,161.983,-78.000,-78.000)
geographic Arctic
Handle The
geographic_facet Arctic
Handle The
genre Arctic
genre_facet Arctic
op_source Biomédica: revista del Instituto Nacional de Salud, Vol 38, Iss 1, Pp 96-104 (2018)
op_relation https://www.revistabiomedica.org/index.php/biomedica/article/view/3653
https://doaj.org/toc/0120-4157
0120-4157
doi:10.7705/biomedica.v38i0.3653
https://doaj.org/article/5869945ea2f5417fb641f7ec7aece704
op_doi https://doi.org/10.7705/biomedica.v38i0.3653
container_title Biomédica
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