Analysis of host- and strain-dependent cell death responses during infectious salmon anemia virus infection in vitro
Abstract Background Infectious salmon anemia virus (ISAV) is an aquatic orthomyxovirus and the causative agent of infectious salmon anemia (ISA), a disease of great importance in the Atlantic salmon farming industry. In vitro , ISAV infection causes cytophatic effect (CPE) in cell lines from Atlanti...
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ftdoajarticles:oai:doaj.org/article:58490705fda34bb79373c18c6e1f897c 2023-05-15T15:30:35+02:00 Analysis of host- and strain-dependent cell death responses during infectious salmon anemia virus infection in vitro Mjaaland Siri Poulsen Lene C Bækkevold Espen S Schiøtz Berit L Gjøen Tor 2009-07-01T00:00:00Z https://doi.org/10.1186/1743-422X-6-91 https://doaj.org/article/58490705fda34bb79373c18c6e1f897c EN eng BMC http://www.virologyj.com/content/6/1/91 https://doaj.org/toc/1743-422X doi:10.1186/1743-422X-6-91 1743-422X https://doaj.org/article/58490705fda34bb79373c18c6e1f897c Virology Journal, Vol 6, Iss 1, p 91 (2009) Infectious and parasitic diseases RC109-216 article 2009 ftdoajarticles https://doi.org/10.1186/1743-422X-6-91 2022-12-31T08:26:40Z Abstract Background Infectious salmon anemia virus (ISAV) is an aquatic orthomyxovirus and the causative agent of infectious salmon anemia (ISA), a disease of great importance in the Atlantic salmon farming industry. In vitro , ISAV infection causes cytophatic effect (CPE) in cell lines from Atlantic salmon, leading to rounding and finally detachment of the cells from the substratum. In this study, we investigated the mode of cell death during in vitro ISAV infection in different Atlantic salmon cell lines, using four ISAV strains causing different mortality in vivo . Results The results show that caspase 3/7 activity increased during the course of infection in ASK and SHK-1 cells, infected cells showed increased surface expression of phosphatidylserine and increased PI uptake, compared to mock infected cells; and morphological alterations of the mitochondria were observed. Expression analysis of immune relevant genes revealed no correlation between in vivo mortality and expression, but good correlation in expression of interferon genes. Conclusion Results from this study indicate that there is both strain and cell type dependent differences in the virus-host interaction during ISAV infection. This is important to bear in mind when extrapolating in vitro findings to the in vivo situation. Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles Virology Journal 6 1 91 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Infectious and parasitic diseases RC109-216 |
spellingShingle |
Infectious and parasitic diseases RC109-216 Mjaaland Siri Poulsen Lene C Bækkevold Espen S Schiøtz Berit L Gjøen Tor Analysis of host- and strain-dependent cell death responses during infectious salmon anemia virus infection in vitro |
topic_facet |
Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Infectious salmon anemia virus (ISAV) is an aquatic orthomyxovirus and the causative agent of infectious salmon anemia (ISA), a disease of great importance in the Atlantic salmon farming industry. In vitro , ISAV infection causes cytophatic effect (CPE) in cell lines from Atlantic salmon, leading to rounding and finally detachment of the cells from the substratum. In this study, we investigated the mode of cell death during in vitro ISAV infection in different Atlantic salmon cell lines, using four ISAV strains causing different mortality in vivo . Results The results show that caspase 3/7 activity increased during the course of infection in ASK and SHK-1 cells, infected cells showed increased surface expression of phosphatidylserine and increased PI uptake, compared to mock infected cells; and morphological alterations of the mitochondria were observed. Expression analysis of immune relevant genes revealed no correlation between in vivo mortality and expression, but good correlation in expression of interferon genes. Conclusion Results from this study indicate that there is both strain and cell type dependent differences in the virus-host interaction during ISAV infection. This is important to bear in mind when extrapolating in vitro findings to the in vivo situation. |
format |
Article in Journal/Newspaper |
author |
Mjaaland Siri Poulsen Lene C Bækkevold Espen S Schiøtz Berit L Gjøen Tor |
author_facet |
Mjaaland Siri Poulsen Lene C Bækkevold Espen S Schiøtz Berit L Gjøen Tor |
author_sort |
Mjaaland Siri |
title |
Analysis of host- and strain-dependent cell death responses during infectious salmon anemia virus infection in vitro |
title_short |
Analysis of host- and strain-dependent cell death responses during infectious salmon anemia virus infection in vitro |
title_full |
Analysis of host- and strain-dependent cell death responses during infectious salmon anemia virus infection in vitro |
title_fullStr |
Analysis of host- and strain-dependent cell death responses during infectious salmon anemia virus infection in vitro |
title_full_unstemmed |
Analysis of host- and strain-dependent cell death responses during infectious salmon anemia virus infection in vitro |
title_sort |
analysis of host- and strain-dependent cell death responses during infectious salmon anemia virus infection in vitro |
publisher |
BMC |
publishDate |
2009 |
url |
https://doi.org/10.1186/1743-422X-6-91 https://doaj.org/article/58490705fda34bb79373c18c6e1f897c |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Virology Journal, Vol 6, Iss 1, p 91 (2009) |
op_relation |
http://www.virologyj.com/content/6/1/91 https://doaj.org/toc/1743-422X doi:10.1186/1743-422X-6-91 1743-422X https://doaj.org/article/58490705fda34bb79373c18c6e1f897c |
op_doi |
https://doi.org/10.1186/1743-422X-6-91 |
container_title |
Virology Journal |
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6 |
container_issue |
1 |
container_start_page |
91 |
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1766361038347829248 |