Scorpions from the primeval subgenus Archaeotityus produce putative homologs of Tityus serrulatus toxins active on voltage-gated sodium channels

• Published in: Journal of Venomous Animals and Toxins including Tropical Diseases
• Main Authors: A Borges, MJ Jowers, S Bónoli, L De Sousa
• Format: Article in Journal/Newspaper
• Language: English
• Published: SciELO 2012
• Subjects: Archaeotityus; scorpions; scorpion toxins; Tityus; Tityus clathratus; Tityus serrulatus; Arctic medicine. Tropical medicine; RC955-962; Toxicology. Poisons; RA1190-1270; Zoology; QL1-991; Arctic
• Online Access: https://doi.org/10.1590/S1678-91992012000400012; https://doaj.org/article/58432835f7af4c5bb116fdcd9958e0ee

It has been proposed that the subgenus Archaeotityus comprises the most ancient species group within the medically important scorpion genus Tityus. cDNA encoding sodium-channel active toxins from the type species of this subgenus, Tityus clathratus (central Venezuela), have been isolated and sequenced. Two cDNAs were retrieved that encoded 61 amino acid-long putative neurotoxins named Tcl1 and Tcl2. Sequence identity was highest (87%) when both were compared with β-toxin Ts1 from the Brazilian scorpion Tityus serrulatus and its homologs from T. bahiensis, T. stigmurus, and T. costatus. A Bayesian analysis indicated statistical support for the grouping of T. clathratus Tcl1 and Tcl2 with Brazilian gamma-like β-toxins, reinforcing previous phylogenetic studies which suggested an evolutionary relationship between the subgenus Archaeotityus and scorpion species inhabiting southeast South America belonging to the subgenus Tityus.