Study on the Effect of Relaying on Norovirus Reduction from Crassostrea gigas Oysters
Norovirus (NoV) is the most important cause of seafood-borne gastroenteritis worldwide, mainly associated with the consumption of raw oysters. NoV is often present in oysters that comply with existing control standards for shellfish. Therefore, the improvement of post-harvest treatments and practice...
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2022
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ftdoajarticles:oai:doaj.org/article:583cace996924006b2829ec3625c1161 2023-05-15T15:58:06+02:00 Study on the Effect of Relaying on Norovirus Reduction from Crassostrea gigas Oysters Roberta Battistini Chiara Masotti Cristiana Maurella Erica Costa Mino Orlandi Mirvana Feletti Carlo Ercolini Laura Serracca 2022-12-01T00:00:00Z https://doi.org/10.3390/microorganisms10122389 https://doaj.org/article/583cace996924006b2829ec3625c1161 EN eng MDPI AG https://www.mdpi.com/2076-2607/10/12/2389 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms10122389 2076-2607 https://doaj.org/article/583cace996924006b2829ec3625c1161 Microorganisms, Vol 10, Iss 2389, p 2389 (2022) Crassostrea gigas relay norovirus food safety real-time qPCR Biology (General) QH301-705.5 article 2022 ftdoajarticles https://doi.org/10.3390/microorganisms10122389 2022-12-30T19:30:56Z Norovirus (NoV) is the most important cause of seafood-borne gastroenteritis worldwide, mainly associated with the consumption of raw oysters. NoV is often present in oysters that comply with existing control standards for shellfish. Therefore, the improvement of post-harvest treatments and practices can represent one of the main strategies to reduce the incidence of viral diseases related to shellfish. This study aimed to investigate long-term relays for the reduction of NoV levels in live oysters, during the high-risk cold months, by transferring the oysters from a more contaminated site to two sites with lower NoV levels. The efficacy of relaying was evaluated by analyzing oyster samples collected at days 0 (T0) and 30 (T30) for NoV levels using a real-time quantitative PCR (RT-qPCR). The NoV level at the relay sites was consistently lower than at the harvest site. The relay process for 30 days in seawater with a lower NoV level resulted in a decrease in the NoV load compared to day 0 with significant reductions depending on the site and genogroup of NoV considered. These results suggest that long-term relaying of oysters to reduce NoV levels is promising and could help growers to improve oyster safety; however, further investigations are needed. Article in Journal/Newspaper Crassostrea gigas Directory of Open Access Journals: DOAJ Articles Microorganisms 10 12 2389 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Crassostrea gigas relay norovirus food safety real-time qPCR Biology (General) QH301-705.5 |
spellingShingle |
Crassostrea gigas relay norovirus food safety real-time qPCR Biology (General) QH301-705.5 Roberta Battistini Chiara Masotti Cristiana Maurella Erica Costa Mino Orlandi Mirvana Feletti Carlo Ercolini Laura Serracca Study on the Effect of Relaying on Norovirus Reduction from Crassostrea gigas Oysters |
topic_facet |
Crassostrea gigas relay norovirus food safety real-time qPCR Biology (General) QH301-705.5 |
description |
Norovirus (NoV) is the most important cause of seafood-borne gastroenteritis worldwide, mainly associated with the consumption of raw oysters. NoV is often present in oysters that comply with existing control standards for shellfish. Therefore, the improvement of post-harvest treatments and practices can represent one of the main strategies to reduce the incidence of viral diseases related to shellfish. This study aimed to investigate long-term relays for the reduction of NoV levels in live oysters, during the high-risk cold months, by transferring the oysters from a more contaminated site to two sites with lower NoV levels. The efficacy of relaying was evaluated by analyzing oyster samples collected at days 0 (T0) and 30 (T30) for NoV levels using a real-time quantitative PCR (RT-qPCR). The NoV level at the relay sites was consistently lower than at the harvest site. The relay process for 30 days in seawater with a lower NoV level resulted in a decrease in the NoV load compared to day 0 with significant reductions depending on the site and genogroup of NoV considered. These results suggest that long-term relaying of oysters to reduce NoV levels is promising and could help growers to improve oyster safety; however, further investigations are needed. |
format |
Article in Journal/Newspaper |
author |
Roberta Battistini Chiara Masotti Cristiana Maurella Erica Costa Mino Orlandi Mirvana Feletti Carlo Ercolini Laura Serracca |
author_facet |
Roberta Battistini Chiara Masotti Cristiana Maurella Erica Costa Mino Orlandi Mirvana Feletti Carlo Ercolini Laura Serracca |
author_sort |
Roberta Battistini |
title |
Study on the Effect of Relaying on Norovirus Reduction from Crassostrea gigas Oysters |
title_short |
Study on the Effect of Relaying on Norovirus Reduction from Crassostrea gigas Oysters |
title_full |
Study on the Effect of Relaying on Norovirus Reduction from Crassostrea gigas Oysters |
title_fullStr |
Study on the Effect of Relaying on Norovirus Reduction from Crassostrea gigas Oysters |
title_full_unstemmed |
Study on the Effect of Relaying on Norovirus Reduction from Crassostrea gigas Oysters |
title_sort |
study on the effect of relaying on norovirus reduction from crassostrea gigas oysters |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/microorganisms10122389 https://doaj.org/article/583cace996924006b2829ec3625c1161 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Microorganisms, Vol 10, Iss 2389, p 2389 (2022) |
op_relation |
https://www.mdpi.com/2076-2607/10/12/2389 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms10122389 2076-2607 https://doaj.org/article/583cace996924006b2829ec3625c1161 |
op_doi |
https://doi.org/10.3390/microorganisms10122389 |
container_title |
Microorganisms |
container_volume |
10 |
container_issue |
12 |
container_start_page |
2389 |
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1766393828882776064 |