Arbitrarily primed PCR fingerprinting of RNA and DNA in Entamoeba histolytica
Differences were detected in the gene expression of strains of E. histolytica using RNA (RAP-PCR) and DNA fingerprinting (RAPD). Analysis of the electrophoretic profiles of the gels revealed some polymorphic markers that could be used in the individual characterization of the strains. The 260 bands...
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Universidade de São Paulo (USP)
2000
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ftdoajarticles:oai:doaj.org/article:5818523333fb415abea105ea487daaf3 2024-09-09T19:25:14+00:00 Arbitrarily primed PCR fingerprinting of RNA and DNA in Entamoeba histolytica Paulo Renato VALLE Maria Betânia G. SOUZA Edna M. PIRES Edward F. SILVA Maria A. GOMES 2000-10-01T00:00:00Z https://doaj.org/article/5818523333fb415abea105ea487daaf3 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652000000500003&tlng=en https://doaj.org/toc/1678-9946 1678-9946 https://doaj.org/article/5818523333fb415abea105ea487daaf3 Revista do Instituto de Medicina Tropical de São Paulo, Vol 42, Iss 5, Pp 249-253 (2000) Entamoeba histolytica Virulence RAP-PCR RAPD Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2000 ftdoajarticles 2024-08-05T17:49:31Z Differences were detected in the gene expression of strains of E. histolytica using RNA (RAP-PCR) and DNA fingerprinting (RAPD). Analysis of the electrophoretic profiles of the gels revealed some polymorphic markers that could be used in the individual characterization of the strains. The 260 bands generated by using five different primers for RAP-PCR, as well as RAPD, were employed in the construction of dendograms. The dendogram obtained based on the RAPD products permitted the distinction of symptomatic and asymptomatic isolates, as well the correlation between the polymorphism exhibited and the virulence of the strains. The dendogram obtained for the RAP-PCR products did not show a correlation with the virulence of the strains but revealed a high degree of intraspecific transcriptional variability that could be related to other biological features, whether or not these are involved in the pathogenesis of amebiasis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Entamoeba histolytica Virulence RAP-PCR RAPD Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Entamoeba histolytica Virulence RAP-PCR RAPD Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Paulo Renato VALLE Maria Betânia G. SOUZA Edna M. PIRES Edward F. SILVA Maria A. GOMES Arbitrarily primed PCR fingerprinting of RNA and DNA in Entamoeba histolytica |
topic_facet |
Entamoeba histolytica Virulence RAP-PCR RAPD Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Differences were detected in the gene expression of strains of E. histolytica using RNA (RAP-PCR) and DNA fingerprinting (RAPD). Analysis of the electrophoretic profiles of the gels revealed some polymorphic markers that could be used in the individual characterization of the strains. The 260 bands generated by using five different primers for RAP-PCR, as well as RAPD, were employed in the construction of dendograms. The dendogram obtained based on the RAPD products permitted the distinction of symptomatic and asymptomatic isolates, as well the correlation between the polymorphism exhibited and the virulence of the strains. The dendogram obtained for the RAP-PCR products did not show a correlation with the virulence of the strains but revealed a high degree of intraspecific transcriptional variability that could be related to other biological features, whether or not these are involved in the pathogenesis of amebiasis. |
format |
Article in Journal/Newspaper |
author |
Paulo Renato VALLE Maria Betânia G. SOUZA Edna M. PIRES Edward F. SILVA Maria A. GOMES |
author_facet |
Paulo Renato VALLE Maria Betânia G. SOUZA Edna M. PIRES Edward F. SILVA Maria A. GOMES |
author_sort |
Paulo Renato VALLE |
title |
Arbitrarily primed PCR fingerprinting of RNA and DNA in Entamoeba histolytica |
title_short |
Arbitrarily primed PCR fingerprinting of RNA and DNA in Entamoeba histolytica |
title_full |
Arbitrarily primed PCR fingerprinting of RNA and DNA in Entamoeba histolytica |
title_fullStr |
Arbitrarily primed PCR fingerprinting of RNA and DNA in Entamoeba histolytica |
title_full_unstemmed |
Arbitrarily primed PCR fingerprinting of RNA and DNA in Entamoeba histolytica |
title_sort |
arbitrarily primed pcr fingerprinting of rna and dna in entamoeba histolytica |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2000 |
url |
https://doaj.org/article/5818523333fb415abea105ea487daaf3 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 42, Iss 5, Pp 249-253 (2000) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652000000500003&tlng=en https://doaj.org/toc/1678-9946 1678-9946 https://doaj.org/article/5818523333fb415abea105ea487daaf3 |
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1809894994880757760 |