Acute Chagas disease induces cerebral microvasculopathy in mice.
Cardiomyopathy is the main clinical form of Chagas disease (CD); however, cerebral manifestations, such as meningoencephalitis, ischemic stroke and cognitive impairment, can also occur. The aim of the present study was to investigate functional microvascular alterations and oxidative stress in the b...
Published in: | PLoS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2014
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0002998 https://doaj.org/article/5805a28f79a242b6a0cad6706d1d2138 |
id |
ftdoajarticles:oai:doaj.org/article:5805a28f79a242b6a0cad6706d1d2138 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:5805a28f79a242b6a0cad6706d1d2138 2023-05-15T15:08:24+02:00 Acute Chagas disease induces cerebral microvasculopathy in mice. Lindice Mitie Nisimura Vanessa Estato Elen Mello de Souza Patricia A Reis Marcos Adriano Lessa Hugo Caire Castro-Faria-Neto Mirian Claudia de Souza Pereira Eduardo Tibiriçá Luciana Ribeiro Garzoni 2014-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002998 https://doaj.org/article/5805a28f79a242b6a0cad6706d1d2138 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4091872?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002998 https://doaj.org/article/5805a28f79a242b6a0cad6706d1d2138 PLoS Neglected Tropical Diseases, Vol 8, Iss 7, p e2998 (2014) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2014 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002998 2022-12-31T16:31:09Z Cardiomyopathy is the main clinical form of Chagas disease (CD); however, cerebral manifestations, such as meningoencephalitis, ischemic stroke and cognitive impairment, can also occur. The aim of the present study was to investigate functional microvascular alterations and oxidative stress in the brain of mice in acute CD. Acute CD was induced in Swiss Webster mice (SWM) with the Y strain of Trypanosoma cruzi (T. cruzi). Cerebral functional capillary density (the number of spontaneously perfused capillaries), leukocyte rolling and adhesion and the microvascular endothelial-dependent response were analyzed over a period of fifteen days using intravital video-microscopy. We also evaluated cerebral oxidative stress with the thiobarbituric acid reactive species TBARS method. Compared with the non-infected group, acute CD significantly induced cerebral functional microvascular alterations, including (i) functional capillary rarefaction, (ii) increased leukocyte rolling and adhesion, (iii) the formation of microvascular platelet-leukocyte aggregates, and (iv) alteration of the endothelial response to acetylcholine. Moreover, cerebral oxidative stress increased in infected animals. We concluded that acute CD in mice induced cerebral microvasculopathy, characterized by a reduced incidence of perfused capillaries, a high number of microvascular platelet-leukocyte aggregates, a marked increase in leukocyte-endothelium interactions and brain arteriolar endothelial dysfunction associated with oxidative stress. These results suggest the involvement of cerebral microcirculation alterations in the neurological manifestations of CD. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic The ''Y'' ENVELOPE(-112.453,-112.453,57.591,57.591) PLoS Neglected Tropical Diseases 8 7 e2998 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Lindice Mitie Nisimura Vanessa Estato Elen Mello de Souza Patricia A Reis Marcos Adriano Lessa Hugo Caire Castro-Faria-Neto Mirian Claudia de Souza Pereira Eduardo Tibiriçá Luciana Ribeiro Garzoni Acute Chagas disease induces cerebral microvasculopathy in mice. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Cardiomyopathy is the main clinical form of Chagas disease (CD); however, cerebral manifestations, such as meningoencephalitis, ischemic stroke and cognitive impairment, can also occur. The aim of the present study was to investigate functional microvascular alterations and oxidative stress in the brain of mice in acute CD. Acute CD was induced in Swiss Webster mice (SWM) with the Y strain of Trypanosoma cruzi (T. cruzi). Cerebral functional capillary density (the number of spontaneously perfused capillaries), leukocyte rolling and adhesion and the microvascular endothelial-dependent response were analyzed over a period of fifteen days using intravital video-microscopy. We also evaluated cerebral oxidative stress with the thiobarbituric acid reactive species TBARS method. Compared with the non-infected group, acute CD significantly induced cerebral functional microvascular alterations, including (i) functional capillary rarefaction, (ii) increased leukocyte rolling and adhesion, (iii) the formation of microvascular platelet-leukocyte aggregates, and (iv) alteration of the endothelial response to acetylcholine. Moreover, cerebral oxidative stress increased in infected animals. We concluded that acute CD in mice induced cerebral microvasculopathy, characterized by a reduced incidence of perfused capillaries, a high number of microvascular platelet-leukocyte aggregates, a marked increase in leukocyte-endothelium interactions and brain arteriolar endothelial dysfunction associated with oxidative stress. These results suggest the involvement of cerebral microcirculation alterations in the neurological manifestations of CD. |
format |
Article in Journal/Newspaper |
author |
Lindice Mitie Nisimura Vanessa Estato Elen Mello de Souza Patricia A Reis Marcos Adriano Lessa Hugo Caire Castro-Faria-Neto Mirian Claudia de Souza Pereira Eduardo Tibiriçá Luciana Ribeiro Garzoni |
author_facet |
Lindice Mitie Nisimura Vanessa Estato Elen Mello de Souza Patricia A Reis Marcos Adriano Lessa Hugo Caire Castro-Faria-Neto Mirian Claudia de Souza Pereira Eduardo Tibiriçá Luciana Ribeiro Garzoni |
author_sort |
Lindice Mitie Nisimura |
title |
Acute Chagas disease induces cerebral microvasculopathy in mice. |
title_short |
Acute Chagas disease induces cerebral microvasculopathy in mice. |
title_full |
Acute Chagas disease induces cerebral microvasculopathy in mice. |
title_fullStr |
Acute Chagas disease induces cerebral microvasculopathy in mice. |
title_full_unstemmed |
Acute Chagas disease induces cerebral microvasculopathy in mice. |
title_sort |
acute chagas disease induces cerebral microvasculopathy in mice. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pntd.0002998 https://doaj.org/article/5805a28f79a242b6a0cad6706d1d2138 |
long_lat |
ENVELOPE(-112.453,-112.453,57.591,57.591) |
geographic |
Arctic The ''Y'' |
geographic_facet |
Arctic The ''Y'' |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 8, Iss 7, p e2998 (2014) |
op_relation |
http://europepmc.org/articles/PMC4091872?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002998 https://doaj.org/article/5805a28f79a242b6a0cad6706d1d2138 |
op_doi |
https://doi.org/10.1371/journal.pntd.0002998 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
8 |
container_issue |
7 |
container_start_page |
e2998 |
_version_ |
1766339776914391040 |