Biological Properties and Genetic Characteristics of Francisella tularensis Strains Isolated in the Territory of the Rostov Region in 2020
Objective of the study was to investigate biological properties and genetic characteristics of tularemia agent strains isolated from natural foci of the Rostov Region in 2020.Materials and methods. Field material from natural foci of the Rostov Region was examined by serological, bacteriological, bi...
Published in: | Problems of Particularly Dangerous Infections |
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Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2021
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Online Access: | https://doi.org/10.21055/0370-1069-2021-3-134-140 https://doaj.org/article/57e7860a37c044d18eede6e76bd54c6b |
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ftdoajarticles:oai:doaj.org/article:57e7860a37c044d18eede6e76bd54c6b 2024-09-15T18:16:38+00:00 Biological Properties and Genetic Characteristics of Francisella tularensis Strains Isolated in the Territory of the Rostov Region in 2020 M. V. Tsimbalistova V. M. Sorokin N. V. Aronova A. S. Anisimova N. L. Pichurina N. I. Pasyukova N. A. Selyanskaya S. O. Vodop’yanov A. S. Vodop’yanov R. V. Pisanov N. V. Pavlovich E. V. Kovalev A. K. Noskov 2021-10-01T00:00:00Z https://doi.org/10.21055/0370-1069-2021-3-134-140 https://doaj.org/article/57e7860a37c044d18eede6e76bd54c6b RU rus Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” https://journal.microbe.ru/jour/article/view/1558 https://doaj.org/toc/0370-1069 https://doaj.org/toc/2658-719X 0370-1069 2658-719X doi:10.21055/0370-1069-2021-3-134-140 https://doaj.org/article/57e7860a37c044d18eede6e76bd54c6b Проблемы особо опасных инфекций, Vol 0, Iss 3, Pp 134-140 (2021) francisella tularensis isolation of strains biological properties indel-typing vntr-typing snp-typing Infectious and parasitic diseases RC109-216 article 2021 ftdoajarticles https://doi.org/10.21055/0370-1069-2021-3-134-140 2024-08-05T17:49:00Z Objective of the study was to investigate biological properties and genetic characteristics of tularemia agent strains isolated from natural foci of the Rostov Region in 2020.Materials and methods. Field material from natural foci of the Rostov Region was examined by serological, bacteriological, biological, and molecular-genetic methods. Cultural-morphological, biochemical, antigenic and pathogenic properties of isolated cultures were studied. Protein profles were obtained through MALDI-TOF MS using mass spectrometer Autoflex speed III Bruker Daltonics and Flex Control of Biotyper software. The genetic characteristics of the strains were determined by VNTR and INDEL typing and SNP analysis.Results and discussion. Six strains of tularemia pathogen were isolated from mouse-like rodents using biological method. The investigation of their biological features and data of PCR analysis and INDEL typing with canonical markers showed that all strains are typical representatives of the Francisella tularensis subsp. holarctica biovar EryR. VNTR typing by six genetic loci revealed that all strains belong to four individual genotypes. The strain isolated in 2020 in the Salsky district was identical to the strain which was isolated in the same area in 1989. Based on the whole genome sequencing of two strains, we established that they are closest to the cultures isolated in Turkey (2009, 2012) and Khanty-Mansiysk (2013) by the studied set of SNP markers. Thus, we found that both identical (or closely related) clones of the tularemia agent and new strains with unique genotypes which previously were not described for the Rostov Region can circulate in natural foci of this region for a long period of time. Article in Journal/Newspaper khanty Directory of Open Access Journals: DOAJ Articles Problems of Particularly Dangerous Infections 3 134 140 |
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francisella tularensis isolation of strains biological properties indel-typing vntr-typing snp-typing Infectious and parasitic diseases RC109-216 |
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francisella tularensis isolation of strains biological properties indel-typing vntr-typing snp-typing Infectious and parasitic diseases RC109-216 M. V. Tsimbalistova V. M. Sorokin N. V. Aronova A. S. Anisimova N. L. Pichurina N. I. Pasyukova N. A. Selyanskaya S. O. Vodop’yanov A. S. Vodop’yanov R. V. Pisanov N. V. Pavlovich E. V. Kovalev A. K. Noskov Biological Properties and Genetic Characteristics of Francisella tularensis Strains Isolated in the Territory of the Rostov Region in 2020 |
topic_facet |
francisella tularensis isolation of strains biological properties indel-typing vntr-typing snp-typing Infectious and parasitic diseases RC109-216 |
description |
Objective of the study was to investigate biological properties and genetic characteristics of tularemia agent strains isolated from natural foci of the Rostov Region in 2020.Materials and methods. Field material from natural foci of the Rostov Region was examined by serological, bacteriological, biological, and molecular-genetic methods. Cultural-morphological, biochemical, antigenic and pathogenic properties of isolated cultures were studied. Protein profles were obtained through MALDI-TOF MS using mass spectrometer Autoflex speed III Bruker Daltonics and Flex Control of Biotyper software. The genetic characteristics of the strains were determined by VNTR and INDEL typing and SNP analysis.Results and discussion. Six strains of tularemia pathogen were isolated from mouse-like rodents using biological method. The investigation of their biological features and data of PCR analysis and INDEL typing with canonical markers showed that all strains are typical representatives of the Francisella tularensis subsp. holarctica biovar EryR. VNTR typing by six genetic loci revealed that all strains belong to four individual genotypes. The strain isolated in 2020 in the Salsky district was identical to the strain which was isolated in the same area in 1989. Based on the whole genome sequencing of two strains, we established that they are closest to the cultures isolated in Turkey (2009, 2012) and Khanty-Mansiysk (2013) by the studied set of SNP markers. Thus, we found that both identical (or closely related) clones of the tularemia agent and new strains with unique genotypes which previously were not described for the Rostov Region can circulate in natural foci of this region for a long period of time. |
format |
Article in Journal/Newspaper |
author |
M. V. Tsimbalistova V. M. Sorokin N. V. Aronova A. S. Anisimova N. L. Pichurina N. I. Pasyukova N. A. Selyanskaya S. O. Vodop’yanov A. S. Vodop’yanov R. V. Pisanov N. V. Pavlovich E. V. Kovalev A. K. Noskov |
author_facet |
M. V. Tsimbalistova V. M. Sorokin N. V. Aronova A. S. Anisimova N. L. Pichurina N. I. Pasyukova N. A. Selyanskaya S. O. Vodop’yanov A. S. Vodop’yanov R. V. Pisanov N. V. Pavlovich E. V. Kovalev A. K. Noskov |
author_sort |
M. V. Tsimbalistova |
title |
Biological Properties and Genetic Characteristics of Francisella tularensis Strains Isolated in the Territory of the Rostov Region in 2020 |
title_short |
Biological Properties and Genetic Characteristics of Francisella tularensis Strains Isolated in the Territory of the Rostov Region in 2020 |
title_full |
Biological Properties and Genetic Characteristics of Francisella tularensis Strains Isolated in the Territory of the Rostov Region in 2020 |
title_fullStr |
Biological Properties and Genetic Characteristics of Francisella tularensis Strains Isolated in the Territory of the Rostov Region in 2020 |
title_full_unstemmed |
Biological Properties and Genetic Characteristics of Francisella tularensis Strains Isolated in the Territory of the Rostov Region in 2020 |
title_sort |
biological properties and genetic characteristics of francisella tularensis strains isolated in the territory of the rostov region in 2020 |
publisher |
Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
publishDate |
2021 |
url |
https://doi.org/10.21055/0370-1069-2021-3-134-140 https://doaj.org/article/57e7860a37c044d18eede6e76bd54c6b |
genre |
khanty |
genre_facet |
khanty |
op_source |
Проблемы особо опасных инфекций, Vol 0, Iss 3, Pp 134-140 (2021) |
op_relation |
https://journal.microbe.ru/jour/article/view/1558 https://doaj.org/toc/0370-1069 https://doaj.org/toc/2658-719X 0370-1069 2658-719X doi:10.21055/0370-1069-2021-3-134-140 https://doaj.org/article/57e7860a37c044d18eede6e76bd54c6b |
op_doi |
https://doi.org/10.21055/0370-1069-2021-3-134-140 |
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Problems of Particularly Dangerous Infections |
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