In vivo evaluation of homeostatic effects of Echis carinatus snake venom in Iran
Abstract Background The venom of the family Viperidae, including the saw-scaled viper, is rich in serine proteinases and metalloproteinases, which affect the nervous system, complementary system, blood coagulation, platelet aggregation and blood pressure. One of the most prominent effects of the sna...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:57b3820e6053400aa2e7b8566759e2a6 2023-05-15T15:14:54+02:00 In vivo evaluation of homeostatic effects of Echis carinatus snake venom in Iran Salmanizadeh Hossein Babaie Mahdi Zolfagharian Hossein 2013-02-01T00:00:00Z https://doi.org/10.1186/1678-9199-19-3 https://doaj.org/article/57b3820e6053400aa2e7b8566759e2a6 EN eng SciELO http://www.jvat.org/content/19/1/4 https://doaj.org/toc/1678-9199 doi:10.1186/1678-9199-19-3 1678-9199 https://doaj.org/article/57b3820e6053400aa2e7b8566759e2a6 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 19, Iss 1, p 3 (2013) Snake venom Procoagulant activity Blood coagulation Echis carinatus Chromatography LD 50 Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2013 ftdoajarticles https://doi.org/10.1186/1678-9199-19-3 2022-12-31T02:56:55Z Abstract Background The venom of the family Viperidae, including the saw-scaled viper, is rich in serine proteinases and metalloproteinases, which affect the nervous system, complementary system, blood coagulation, platelet aggregation and blood pressure. One of the most prominent effects of the snake venom of Echis carinatus (Ec) is its coagulation activity, used for killing prey. Materials and methods Subfractions F1A and F1B were isolated from Ec crude venom by a combination of gel chromatography (Sephadex G-75) and ion exchange chromatography on a DEAE-Sepharose (DE-52). These subfractions were then intravenously (IV) injected into NIH male mice. Blood samples were taken before and after the administration of these subfractions. Times for prothrombin, partial thromboplastin and fibrinogen were recorded. Results and conclusions Comparison of the prothrombin time before and after F1A and F1B administrations showed that time for blood coagulation after injection is shorter than that of normal blood coagulation and also reduced coagulation time after Ec crude venom injection. This difference in coagulation time shows the intense coagulation activity of these subfractions that significantly increase the coagulation cascade rate and Causes to quick blood coagulation. The LD50 of the Ec crude venom was also determined to be 11.1 μg/mouse. Different crude venom doses were prepared with physiological serum and injected into four mice. Comparison of the prothrombin times after injection of subfractions F1A and F1B showed that the rate of mouse blood coagulation increases considerably. Comparing the partial thromboplastin times after injecting these subfractions with this normal test time showed that the activity rate of intrinsic blood coagulation system rose sharply in mice. Finally, by comparing the fibrinogen time after subfraction injections and normal test time, we can infer intense activation of coagulation cascade and fibrin production. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 19 1 3 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Snake venom Procoagulant activity Blood coagulation Echis carinatus Chromatography LD 50 Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Snake venom Procoagulant activity Blood coagulation Echis carinatus Chromatography LD 50 Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Salmanizadeh Hossein Babaie Mahdi Zolfagharian Hossein In vivo evaluation of homeostatic effects of Echis carinatus snake venom in Iran |
topic_facet |
Snake venom Procoagulant activity Blood coagulation Echis carinatus Chromatography LD 50 Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background The venom of the family Viperidae, including the saw-scaled viper, is rich in serine proteinases and metalloproteinases, which affect the nervous system, complementary system, blood coagulation, platelet aggregation and blood pressure. One of the most prominent effects of the snake venom of Echis carinatus (Ec) is its coagulation activity, used for killing prey. Materials and methods Subfractions F1A and F1B were isolated from Ec crude venom by a combination of gel chromatography (Sephadex G-75) and ion exchange chromatography on a DEAE-Sepharose (DE-52). These subfractions were then intravenously (IV) injected into NIH male mice. Blood samples were taken before and after the administration of these subfractions. Times for prothrombin, partial thromboplastin and fibrinogen were recorded. Results and conclusions Comparison of the prothrombin time before and after F1A and F1B administrations showed that time for blood coagulation after injection is shorter than that of normal blood coagulation and also reduced coagulation time after Ec crude venom injection. This difference in coagulation time shows the intense coagulation activity of these subfractions that significantly increase the coagulation cascade rate and Causes to quick blood coagulation. The LD50 of the Ec crude venom was also determined to be 11.1 μg/mouse. Different crude venom doses were prepared with physiological serum and injected into four mice. Comparison of the prothrombin times after injection of subfractions F1A and F1B showed that the rate of mouse blood coagulation increases considerably. Comparing the partial thromboplastin times after injecting these subfractions with this normal test time showed that the activity rate of intrinsic blood coagulation system rose sharply in mice. Finally, by comparing the fibrinogen time after subfraction injections and normal test time, we can infer intense activation of coagulation cascade and fibrin production. |
format |
Article in Journal/Newspaper |
author |
Salmanizadeh Hossein Babaie Mahdi Zolfagharian Hossein |
author_facet |
Salmanizadeh Hossein Babaie Mahdi Zolfagharian Hossein |
author_sort |
Salmanizadeh Hossein |
title |
In vivo evaluation of homeostatic effects of Echis carinatus snake venom in Iran |
title_short |
In vivo evaluation of homeostatic effects of Echis carinatus snake venom in Iran |
title_full |
In vivo evaluation of homeostatic effects of Echis carinatus snake venom in Iran |
title_fullStr |
In vivo evaluation of homeostatic effects of Echis carinatus snake venom in Iran |
title_full_unstemmed |
In vivo evaluation of homeostatic effects of Echis carinatus snake venom in Iran |
title_sort |
in vivo evaluation of homeostatic effects of echis carinatus snake venom in iran |
publisher |
SciELO |
publishDate |
2013 |
url |
https://doi.org/10.1186/1678-9199-19-3 https://doaj.org/article/57b3820e6053400aa2e7b8566759e2a6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 19, Iss 1, p 3 (2013) |
op_relation |
http://www.jvat.org/content/19/1/4 https://doaj.org/toc/1678-9199 doi:10.1186/1678-9199-19-3 1678-9199 https://doaj.org/article/57b3820e6053400aa2e7b8566759e2a6 |
op_doi |
https://doi.org/10.1186/1678-9199-19-3 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
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19 |
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1 |
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3 |
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1766345291896717312 |