Histochemical and Immunohistochemical Characterizations of Hepatic Trematodiasis in Odontocetes
Hepatic trematodiasis is a common condition in a number of free-ranging cetacean species, which occasionally result in severe hepatic and/or pancreatic lesions. However, even the basic pathological information of this disease is unknown for the majority of affected species. The current study describ...
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ftdoajarticles:oai:doaj.org/article:579a2bebdf6147a9b4f1833505077b61 2023-05-15T17:59:14+02:00 Histochemical and Immunohistochemical Characterizations of Hepatic Trematodiasis in Odontocetes Shotaro Nakagun Yoshiyasu Kobayashi 2020-06-01T00:00:00Z https://doi.org/10.3389/fvets.2020.00336 https://doaj.org/article/579a2bebdf6147a9b4f1833505077b61 EN eng Frontiers Media S.A. https://www.frontiersin.org/article/10.3389/fvets.2020.00336/full https://doaj.org/toc/2297-1769 2297-1769 doi:10.3389/fvets.2020.00336 https://doaj.org/article/579a2bebdf6147a9b4f1833505077b61 Frontiers in Veterinary Science, Vol 7 (2020) cetacean liver fluke pathology parasite macrophage Veterinary medicine SF600-1100 article 2020 ftdoajarticles https://doi.org/10.3389/fvets.2020.00336 2022-12-31T00:52:17Z Hepatic trematodiasis is a common condition in a number of free-ranging cetacean species, which occasionally result in severe hepatic and/or pancreatic lesions. However, even the basic pathological information of this disease is unknown for the majority of affected species. The current study describes and compares the histomorphology and immune reaction induced by hepatic trematodes of the family Brachycladiidae in the liver of the harbor porpoise (Phocoena phocoena, n = 8), Dall's porpoise (Phocoenoides dalli, n = 8), and Hubbs' beaked whale (Mesoplodon carlhubbsi, n = 2). Immunohistochemistry for eight antibodies (CK19, CD3, Foxp3, CD20, Iba1, CD68, CD163, and CD204) was conducted to analyze the pathology of these parasitic infections. In all three odontocete species, the changes observed in the trematode-affected biliary epithelium were comparable with marked hyperplasia and goblet cell metaplasia, as well as lymphoplasmacytic and eosinophilic inflammation. Additionally, regions of the Glisson's sheath were diffusely and severely fibrotic in all examined species, regardless of the physical presence of trematodes. Differences among the three species included the presence of characteristic lymphoid follicles formed in the fibrotic bile duct walls of only the two porpoise species. In the Hubbs' beaked whale, the degree of lymphoplasmacytic cholangitis was more severe, and ductular reaction was generally more prominent. In terms of the overall macrophage population among the three species, CD163- and CD204-positive cells (M2 macrophages) outnumbered Iba1- and CD68-positive cells (M1 macrophages), indicating a chronic infection stage in all analyzed individuals. Species-specific differences among the infiltrating macrophages included numbers of CD68-positive cells being significantly more abundant in the harbor porpoises, whereas CD163-positive cells were significantly more numerous in the Dall's porpoises. The numbers of CD204-positive macrophages were higher in the Hubbs' beaked whales compared to those in the ... Article in Journal/Newspaper Phocoena phocoena Directory of Open Access Journals: DOAJ Articles Frontiers in Veterinary Science 7 |
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topic |
cetacean liver fluke pathology parasite macrophage Veterinary medicine SF600-1100 |
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cetacean liver fluke pathology parasite macrophage Veterinary medicine SF600-1100 Shotaro Nakagun Yoshiyasu Kobayashi Histochemical and Immunohistochemical Characterizations of Hepatic Trematodiasis in Odontocetes |
topic_facet |
cetacean liver fluke pathology parasite macrophage Veterinary medicine SF600-1100 |
description |
Hepatic trematodiasis is a common condition in a number of free-ranging cetacean species, which occasionally result in severe hepatic and/or pancreatic lesions. However, even the basic pathological information of this disease is unknown for the majority of affected species. The current study describes and compares the histomorphology and immune reaction induced by hepatic trematodes of the family Brachycladiidae in the liver of the harbor porpoise (Phocoena phocoena, n = 8), Dall's porpoise (Phocoenoides dalli, n = 8), and Hubbs' beaked whale (Mesoplodon carlhubbsi, n = 2). Immunohistochemistry for eight antibodies (CK19, CD3, Foxp3, CD20, Iba1, CD68, CD163, and CD204) was conducted to analyze the pathology of these parasitic infections. In all three odontocete species, the changes observed in the trematode-affected biliary epithelium were comparable with marked hyperplasia and goblet cell metaplasia, as well as lymphoplasmacytic and eosinophilic inflammation. Additionally, regions of the Glisson's sheath were diffusely and severely fibrotic in all examined species, regardless of the physical presence of trematodes. Differences among the three species included the presence of characteristic lymphoid follicles formed in the fibrotic bile duct walls of only the two porpoise species. In the Hubbs' beaked whale, the degree of lymphoplasmacytic cholangitis was more severe, and ductular reaction was generally more prominent. In terms of the overall macrophage population among the three species, CD163- and CD204-positive cells (M2 macrophages) outnumbered Iba1- and CD68-positive cells (M1 macrophages), indicating a chronic infection stage in all analyzed individuals. Species-specific differences among the infiltrating macrophages included numbers of CD68-positive cells being significantly more abundant in the harbor porpoises, whereas CD163-positive cells were significantly more numerous in the Dall's porpoises. The numbers of CD204-positive macrophages were higher in the Hubbs' beaked whales compared to those in the ... |
format |
Article in Journal/Newspaper |
author |
Shotaro Nakagun Yoshiyasu Kobayashi |
author_facet |
Shotaro Nakagun Yoshiyasu Kobayashi |
author_sort |
Shotaro Nakagun |
title |
Histochemical and Immunohistochemical Characterizations of Hepatic Trematodiasis in Odontocetes |
title_short |
Histochemical and Immunohistochemical Characterizations of Hepatic Trematodiasis in Odontocetes |
title_full |
Histochemical and Immunohistochemical Characterizations of Hepatic Trematodiasis in Odontocetes |
title_fullStr |
Histochemical and Immunohistochemical Characterizations of Hepatic Trematodiasis in Odontocetes |
title_full_unstemmed |
Histochemical and Immunohistochemical Characterizations of Hepatic Trematodiasis in Odontocetes |
title_sort |
histochemical and immunohistochemical characterizations of hepatic trematodiasis in odontocetes |
publisher |
Frontiers Media S.A. |
publishDate |
2020 |
url |
https://doi.org/10.3389/fvets.2020.00336 https://doaj.org/article/579a2bebdf6147a9b4f1833505077b61 |
genre |
Phocoena phocoena |
genre_facet |
Phocoena phocoena |
op_source |
Frontiers in Veterinary Science, Vol 7 (2020) |
op_relation |
https://www.frontiersin.org/article/10.3389/fvets.2020.00336/full https://doaj.org/toc/2297-1769 2297-1769 doi:10.3389/fvets.2020.00336 https://doaj.org/article/579a2bebdf6147a9b4f1833505077b61 |
op_doi |
https://doi.org/10.3389/fvets.2020.00336 |
container_title |
Frontiers in Veterinary Science |
container_volume |
7 |
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1766168011895472128 |