Molecular Characterization of Cryptosporidium Species and Giardia duodenalis from Symptomatic Cambodian Children.
In a prospective study, 498 single faecal samples from children aged under 16 years attending an outpatient clinic in the Angkor Hospital for Children, northwest Cambodia, were examined for Cryptosporidium oocysts and Giardia cysts using microscopy and molecular assays.Cryptosporidium oocysts were d...
Published in: | PLOS Neglected Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:57944d1334614f539c5c394c08cf2551 2023-05-15T15:15:22+02:00 Molecular Characterization of Cryptosporidium Species and Giardia duodenalis from Symptomatic Cambodian Children. Catrin E Moore Kristin Elwin Nget Phot Chanthou Seng Saroeun Mao Kuong Suy Varun Kumar Johanna Nader Rachel Bousfield Sanuki Perera J Wendi Bailey Nicholas J Beeching Nicholas P J Day Christopher M Parry Rachel M Chalmers 2016-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004822 https://doaj.org/article/57944d1334614f539c5c394c08cf2551 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4936737?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004822 https://doaj.org/article/57944d1334614f539c5c394c08cf2551 PLoS Neglected Tropical Diseases, Vol 10, Iss 7, p e0004822 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004822 2022-12-31T11:12:40Z In a prospective study, 498 single faecal samples from children aged under 16 years attending an outpatient clinic in the Angkor Hospital for Children, northwest Cambodia, were examined for Cryptosporidium oocysts and Giardia cysts using microscopy and molecular assays.Cryptosporidium oocysts were detected in 2.2% (11/498) of samples using microscopy and in 7.7% (38/498) with molecular tests. Giardia duodenalis cysts were detected in 18.9% (94/498) by microscopy and 27.7% (138/498) by molecular tests; 82% of the positive samples (by either method) were from children aged 1-10 years. Cryptosporidium hominis was the most common species of Cryptosporidium, detected in 13 (34.2%) samples, followed by Cryptosporidium meleagridis in 9 (23.7%), Cryptosporidium parvum in 8 (21.1%), Cryptosporidium canis in 5 (13.2%), and Cryptosporidium suis and Cryptosporidium ubiquitum in one sample each. Cryptosporidium hominis and C. parvum positive samples were subtyped by sequencing the GP60 gene: C. hominis IaA16R6 and C. parvum IIeA7G1 were the most abundant subtypes. Giardia duodenalis was typed using a multiplex real-time PCR targeting assemblages A and B. Assemblage B (106; 76.8% of all Giardia positive samples) was most common followed by A (12.3%) and mixed infections (5.1%). Risk factors associated with Cryptosporidium were malnutrition (AOR 9.63, 95% CI 1.67-55.46), chronic medical diagnoses (AOR 4.51, 95% CI 1.79-11.34) and the presence of birds in the household (AOR 2.99, 95% CI 1.16-7.73); specifically C. hominis (p = 0.03) and C. meleagridis (p<0.001) were associated with the presence of birds. The use of soap was protective against Giardia infection (OR 0.74, 95% CI 0.58-0.95).This is the first report to describe the different Cryptosporidium species and subtypes and Giardia duodenalis assemblages in Cambodian children. The variety of Cryptosporidium species detected indicates both anthroponotic and zoonotic transmission in this population. Interventions to improve sanitation, increase hand washing after ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 10 7 e0004822 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Catrin E Moore Kristin Elwin Nget Phot Chanthou Seng Saroeun Mao Kuong Suy Varun Kumar Johanna Nader Rachel Bousfield Sanuki Perera J Wendi Bailey Nicholas J Beeching Nicholas P J Day Christopher M Parry Rachel M Chalmers Molecular Characterization of Cryptosporidium Species and Giardia duodenalis from Symptomatic Cambodian Children. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
In a prospective study, 498 single faecal samples from children aged under 16 years attending an outpatient clinic in the Angkor Hospital for Children, northwest Cambodia, were examined for Cryptosporidium oocysts and Giardia cysts using microscopy and molecular assays.Cryptosporidium oocysts were detected in 2.2% (11/498) of samples using microscopy and in 7.7% (38/498) with molecular tests. Giardia duodenalis cysts were detected in 18.9% (94/498) by microscopy and 27.7% (138/498) by molecular tests; 82% of the positive samples (by either method) were from children aged 1-10 years. Cryptosporidium hominis was the most common species of Cryptosporidium, detected in 13 (34.2%) samples, followed by Cryptosporidium meleagridis in 9 (23.7%), Cryptosporidium parvum in 8 (21.1%), Cryptosporidium canis in 5 (13.2%), and Cryptosporidium suis and Cryptosporidium ubiquitum in one sample each. Cryptosporidium hominis and C. parvum positive samples were subtyped by sequencing the GP60 gene: C. hominis IaA16R6 and C. parvum IIeA7G1 were the most abundant subtypes. Giardia duodenalis was typed using a multiplex real-time PCR targeting assemblages A and B. Assemblage B (106; 76.8% of all Giardia positive samples) was most common followed by A (12.3%) and mixed infections (5.1%). Risk factors associated with Cryptosporidium were malnutrition (AOR 9.63, 95% CI 1.67-55.46), chronic medical diagnoses (AOR 4.51, 95% CI 1.79-11.34) and the presence of birds in the household (AOR 2.99, 95% CI 1.16-7.73); specifically C. hominis (p = 0.03) and C. meleagridis (p<0.001) were associated with the presence of birds. The use of soap was protective against Giardia infection (OR 0.74, 95% CI 0.58-0.95).This is the first report to describe the different Cryptosporidium species and subtypes and Giardia duodenalis assemblages in Cambodian children. The variety of Cryptosporidium species detected indicates both anthroponotic and zoonotic transmission in this population. Interventions to improve sanitation, increase hand washing after ... |
format |
Article in Journal/Newspaper |
author |
Catrin E Moore Kristin Elwin Nget Phot Chanthou Seng Saroeun Mao Kuong Suy Varun Kumar Johanna Nader Rachel Bousfield Sanuki Perera J Wendi Bailey Nicholas J Beeching Nicholas P J Day Christopher M Parry Rachel M Chalmers |
author_facet |
Catrin E Moore Kristin Elwin Nget Phot Chanthou Seng Saroeun Mao Kuong Suy Varun Kumar Johanna Nader Rachel Bousfield Sanuki Perera J Wendi Bailey Nicholas J Beeching Nicholas P J Day Christopher M Parry Rachel M Chalmers |
author_sort |
Catrin E Moore |
title |
Molecular Characterization of Cryptosporidium Species and Giardia duodenalis from Symptomatic Cambodian Children. |
title_short |
Molecular Characterization of Cryptosporidium Species and Giardia duodenalis from Symptomatic Cambodian Children. |
title_full |
Molecular Characterization of Cryptosporidium Species and Giardia duodenalis from Symptomatic Cambodian Children. |
title_fullStr |
Molecular Characterization of Cryptosporidium Species and Giardia duodenalis from Symptomatic Cambodian Children. |
title_full_unstemmed |
Molecular Characterization of Cryptosporidium Species and Giardia duodenalis from Symptomatic Cambodian Children. |
title_sort |
molecular characterization of cryptosporidium species and giardia duodenalis from symptomatic cambodian children. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0004822 https://doaj.org/article/57944d1334614f539c5c394c08cf2551 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 7, p e0004822 (2016) |
op_relation |
http://europepmc.org/articles/PMC4936737?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004822 https://doaj.org/article/57944d1334614f539c5c394c08cf2551 |
op_doi |
https://doi.org/10.1371/journal.pntd.0004822 |
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PLOS Neglected Tropical Diseases |
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10 |
container_issue |
7 |
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e0004822 |
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