Transcriptomic Analysis and Time to Hatch Visual Prediction of Embryo Development in the Ornate Spiny Lobster (Panulirus ornatus)
The ability to conduct closed life-cycle culture of tropical spiny lobsters, Panulirus ornatus, at the Institute for Marine and Antarctic Studies (IMAS) provides a unique opportunity to investigate specific developmental stages during embryogenesis. The production of closed life-cycle lobsters provi...
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ftdoajarticles:oai:doaj.org/article:5746ee3f6b874e4e835654ee8388b672 2023-05-15T14:02:40+02:00 Transcriptomic Analysis and Time to Hatch Visual Prediction of Embryo Development in the Ornate Spiny Lobster (Panulirus ornatus) Courtney L. Lewis Quinn P. Fitzgibbon Gregory G. Smith Abigail Elizur Tomer Ventura 2022-07-01T00:00:00Z https://doi.org/10.3389/fmars.2022.889317 https://doaj.org/article/5746ee3f6b874e4e835654ee8388b672 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fmars.2022.889317/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2022.889317 https://doaj.org/article/5746ee3f6b874e4e835654ee8388b672 Frontiers in Marine Science, Vol 9 (2022) Panulirus ornatus transcriptomics embryogenesis gastrula neuropeptides hatch prediction Science Q General. Including nature conservation geographical distribution QH1-199.5 article 2022 ftdoajarticles https://doi.org/10.3389/fmars.2022.889317 2022-12-31T02:00:52Z The ability to conduct closed life-cycle culture of tropical spiny lobsters, Panulirus ornatus, at the Institute for Marine and Antarctic Studies (IMAS) provides a unique opportunity to investigate specific developmental stages during embryogenesis. The production of closed life-cycle lobsters provides access to embryos at defined time points, yet physical staging is challenging due to their small size and environmental factors impacting their development. A transcriptome comprising 11 distinct stages across the 30-day P. ornatus embryonal development period allowed the establishment of the most detailed transcriptomic library of embryogenesis across decapods. A series of key genes across the 11 stages of embryonal development were characterized. The expression of neuropeptides was reported across P. ornatus embryos, suggesting they are primarily active during the later stages of embryogenesis as the nervous system develops and the animal prepares to hatch. Gastrula-specific genes, nanos and brachyury, presented an expression profile indicating gastrulation occurs early in embryogenesis. In addition to the molecular tools used to characterize embryo development, certain developmental characteristics, such as eye spot development, provide a measurable indicator that can be visualised. Hatch prediction models based on visual characteristics were shown to be an accurate method to predict the timing of the hatch for P. ornatus embryos kept at 26°C. The combination of morphological and visual measures with transcriptomics can be used to further define and establish the groundwork for future characterisation and staging of P. ornatus embryogenesis. Article in Journal/Newspaper Antarc* Antarctic Institute for Marine and Antarctic Studies Directory of Open Access Journals: DOAJ Articles Antarctic Frontiers in Marine Science 9 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Panulirus ornatus transcriptomics embryogenesis gastrula neuropeptides hatch prediction Science Q General. Including nature conservation geographical distribution QH1-199.5 |
spellingShingle |
Panulirus ornatus transcriptomics embryogenesis gastrula neuropeptides hatch prediction Science Q General. Including nature conservation geographical distribution QH1-199.5 Courtney L. Lewis Quinn P. Fitzgibbon Gregory G. Smith Abigail Elizur Tomer Ventura Transcriptomic Analysis and Time to Hatch Visual Prediction of Embryo Development in the Ornate Spiny Lobster (Panulirus ornatus) |
topic_facet |
Panulirus ornatus transcriptomics embryogenesis gastrula neuropeptides hatch prediction Science Q General. Including nature conservation geographical distribution QH1-199.5 |
description |
The ability to conduct closed life-cycle culture of tropical spiny lobsters, Panulirus ornatus, at the Institute for Marine and Antarctic Studies (IMAS) provides a unique opportunity to investigate specific developmental stages during embryogenesis. The production of closed life-cycle lobsters provides access to embryos at defined time points, yet physical staging is challenging due to their small size and environmental factors impacting their development. A transcriptome comprising 11 distinct stages across the 30-day P. ornatus embryonal development period allowed the establishment of the most detailed transcriptomic library of embryogenesis across decapods. A series of key genes across the 11 stages of embryonal development were characterized. The expression of neuropeptides was reported across P. ornatus embryos, suggesting they are primarily active during the later stages of embryogenesis as the nervous system develops and the animal prepares to hatch. Gastrula-specific genes, nanos and brachyury, presented an expression profile indicating gastrulation occurs early in embryogenesis. In addition to the molecular tools used to characterize embryo development, certain developmental characteristics, such as eye spot development, provide a measurable indicator that can be visualised. Hatch prediction models based on visual characteristics were shown to be an accurate method to predict the timing of the hatch for P. ornatus embryos kept at 26°C. The combination of morphological and visual measures with transcriptomics can be used to further define and establish the groundwork for future characterisation and staging of P. ornatus embryogenesis. |
format |
Article in Journal/Newspaper |
author |
Courtney L. Lewis Quinn P. Fitzgibbon Gregory G. Smith Abigail Elizur Tomer Ventura |
author_facet |
Courtney L. Lewis Quinn P. Fitzgibbon Gregory G. Smith Abigail Elizur Tomer Ventura |
author_sort |
Courtney L. Lewis |
title |
Transcriptomic Analysis and Time to Hatch Visual Prediction of Embryo Development in the Ornate Spiny Lobster (Panulirus ornatus) |
title_short |
Transcriptomic Analysis and Time to Hatch Visual Prediction of Embryo Development in the Ornate Spiny Lobster (Panulirus ornatus) |
title_full |
Transcriptomic Analysis and Time to Hatch Visual Prediction of Embryo Development in the Ornate Spiny Lobster (Panulirus ornatus) |
title_fullStr |
Transcriptomic Analysis and Time to Hatch Visual Prediction of Embryo Development in the Ornate Spiny Lobster (Panulirus ornatus) |
title_full_unstemmed |
Transcriptomic Analysis and Time to Hatch Visual Prediction of Embryo Development in the Ornate Spiny Lobster (Panulirus ornatus) |
title_sort |
transcriptomic analysis and time to hatch visual prediction of embryo development in the ornate spiny lobster (panulirus ornatus) |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
https://doi.org/10.3389/fmars.2022.889317 https://doaj.org/article/5746ee3f6b874e4e835654ee8388b672 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Institute for Marine and Antarctic Studies |
genre_facet |
Antarc* Antarctic Institute for Marine and Antarctic Studies |
op_source |
Frontiers in Marine Science, Vol 9 (2022) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fmars.2022.889317/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2022.889317 https://doaj.org/article/5746ee3f6b874e4e835654ee8388b672 |
op_doi |
https://doi.org/10.3389/fmars.2022.889317 |
container_title |
Frontiers in Marine Science |
container_volume |
9 |
_version_ |
1766273022471176192 |