Antimicrobial Activity of Small Synthetic Peptides Based on the Marine Peptide Turgencin A: Prediction of Antimicrobial Peptide Sequences in a Natural Peptide and Strategy for Optimization of Potency
Turgencin A, a potent antimicrobial peptide isolated from the Arctic sea squirt Synoicum turgens , consists of 36 amino acid residues and three disulfide bridges, making it challenging to synthesize. The aim of the present study was to develop a truncated peptide with an antimicrobial drug lead pote...
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ftdoajarticles:oai:doaj.org/article:571f098cccc94f44a4be8cdbb2b6dc26 2023-05-15T14:58:36+02:00 Antimicrobial Activity of Small Synthetic Peptides Based on the Marine Peptide Turgencin A: Prediction of Antimicrobial Peptide Sequences in a Natural Peptide and Strategy for Optimization of Potency Ida K. Ø. Hansen Tomas Lövdahl Danijela Simonovic Kine Ø. Hansen Aaron J. C. Andersen Hege Devold Céline S. M. Richard Jeanette H. Andersen Morten B. Strøm Tor Haug 2020-07-01T00:00:00Z https://doi.org/10.3390/ijms21155460 https://doaj.org/article/571f098cccc94f44a4be8cdbb2b6dc26 EN eng MDPI AG https://www.mdpi.com/1422-0067/21/15/5460 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms21155460 1422-0067 1661-6596 https://doaj.org/article/571f098cccc94f44a4be8cdbb2b6dc26 International Journal of Molecular Sciences, Vol 21, Iss 5460, p 5460 (2020) Arctic ascidian antimicrobial synthetic peptide Synoicum turgens Biology (General) QH301-705.5 Chemistry QD1-999 article 2020 ftdoajarticles https://doi.org/10.3390/ijms21155460 2022-12-31T00:44:33Z Turgencin A, a potent antimicrobial peptide isolated from the Arctic sea squirt Synoicum turgens , consists of 36 amino acid residues and three disulfide bridges, making it challenging to synthesize. The aim of the present study was to develop a truncated peptide with an antimicrobial drug lead potential based on turgencin A. The experiments consisted of: (1) sequence analysis and prediction of antimicrobial potential of truncated 10-mer sequences; (2) synthesis and antimicrobial screening of a lead peptide devoid of the cysteine residues; (3) optimization of in vitro antimicrobial activity of the lead peptide using an amino acid replacement strategy; and (4) screening the synthesized peptides for cytotoxic activities. In silico analysis of turgencin A using various prediction software indicated an internal, cationic 10-mer sequence to be putatively antimicrobial. The synthesized truncated lead peptide displayed weak antimicrobial activity. However, by following a systematic amino acid replacement strategy, a modified peptide was developed that retained the potency of the original peptide. The optimized peptide StAMP-9 displayed bactericidal activity, with minimal inhibitory concentrations of 7.8 µg/mL against Staphylococcus aureus and 3.9 µg/mL against Escherichia coli , and no cytotoxic effects against mammalian cells. Preliminary experiments indicate the bacterial membranes as immediate and primary targets. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic International Journal of Molecular Sciences 21 15 5460 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic ascidian antimicrobial synthetic peptide Synoicum turgens Biology (General) QH301-705.5 Chemistry QD1-999 |
spellingShingle |
Arctic ascidian antimicrobial synthetic peptide Synoicum turgens Biology (General) QH301-705.5 Chemistry QD1-999 Ida K. Ø. Hansen Tomas Lövdahl Danijela Simonovic Kine Ø. Hansen Aaron J. C. Andersen Hege Devold Céline S. M. Richard Jeanette H. Andersen Morten B. Strøm Tor Haug Antimicrobial Activity of Small Synthetic Peptides Based on the Marine Peptide Turgencin A: Prediction of Antimicrobial Peptide Sequences in a Natural Peptide and Strategy for Optimization of Potency |
topic_facet |
Arctic ascidian antimicrobial synthetic peptide Synoicum turgens Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
Turgencin A, a potent antimicrobial peptide isolated from the Arctic sea squirt Synoicum turgens , consists of 36 amino acid residues and three disulfide bridges, making it challenging to synthesize. The aim of the present study was to develop a truncated peptide with an antimicrobial drug lead potential based on turgencin A. The experiments consisted of: (1) sequence analysis and prediction of antimicrobial potential of truncated 10-mer sequences; (2) synthesis and antimicrobial screening of a lead peptide devoid of the cysteine residues; (3) optimization of in vitro antimicrobial activity of the lead peptide using an amino acid replacement strategy; and (4) screening the synthesized peptides for cytotoxic activities. In silico analysis of turgencin A using various prediction software indicated an internal, cationic 10-mer sequence to be putatively antimicrobial. The synthesized truncated lead peptide displayed weak antimicrobial activity. However, by following a systematic amino acid replacement strategy, a modified peptide was developed that retained the potency of the original peptide. The optimized peptide StAMP-9 displayed bactericidal activity, with minimal inhibitory concentrations of 7.8 µg/mL against Staphylococcus aureus and 3.9 µg/mL against Escherichia coli , and no cytotoxic effects against mammalian cells. Preliminary experiments indicate the bacterial membranes as immediate and primary targets. |
format |
Article in Journal/Newspaper |
author |
Ida K. Ø. Hansen Tomas Lövdahl Danijela Simonovic Kine Ø. Hansen Aaron J. C. Andersen Hege Devold Céline S. M. Richard Jeanette H. Andersen Morten B. Strøm Tor Haug |
author_facet |
Ida K. Ø. Hansen Tomas Lövdahl Danijela Simonovic Kine Ø. Hansen Aaron J. C. Andersen Hege Devold Céline S. M. Richard Jeanette H. Andersen Morten B. Strøm Tor Haug |
author_sort |
Ida K. Ø. Hansen |
title |
Antimicrobial Activity of Small Synthetic Peptides Based on the Marine Peptide Turgencin A: Prediction of Antimicrobial Peptide Sequences in a Natural Peptide and Strategy for Optimization of Potency |
title_short |
Antimicrobial Activity of Small Synthetic Peptides Based on the Marine Peptide Turgencin A: Prediction of Antimicrobial Peptide Sequences in a Natural Peptide and Strategy for Optimization of Potency |
title_full |
Antimicrobial Activity of Small Synthetic Peptides Based on the Marine Peptide Turgencin A: Prediction of Antimicrobial Peptide Sequences in a Natural Peptide and Strategy for Optimization of Potency |
title_fullStr |
Antimicrobial Activity of Small Synthetic Peptides Based on the Marine Peptide Turgencin A: Prediction of Antimicrobial Peptide Sequences in a Natural Peptide and Strategy for Optimization of Potency |
title_full_unstemmed |
Antimicrobial Activity of Small Synthetic Peptides Based on the Marine Peptide Turgencin A: Prediction of Antimicrobial Peptide Sequences in a Natural Peptide and Strategy for Optimization of Potency |
title_sort |
antimicrobial activity of small synthetic peptides based on the marine peptide turgencin a: prediction of antimicrobial peptide sequences in a natural peptide and strategy for optimization of potency |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/ijms21155460 https://doaj.org/article/571f098cccc94f44a4be8cdbb2b6dc26 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
International Journal of Molecular Sciences, Vol 21, Iss 5460, p 5460 (2020) |
op_relation |
https://www.mdpi.com/1422-0067/21/15/5460 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms21155460 1422-0067 1661-6596 https://doaj.org/article/571f098cccc94f44a4be8cdbb2b6dc26 |
op_doi |
https://doi.org/10.3390/ijms21155460 |
container_title |
International Journal of Molecular Sciences |
container_volume |
21 |
container_issue |
15 |
container_start_page |
5460 |
_version_ |
1766330730932076544 |