Antioxidant capacity and genoprotective effect of ethanol fruit extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae)
Objective: To evidence the ability of ethanol fruit extract from Detarium microcarpum (D. microcarpum) to preserve DNA integrity against oxidative genomic damage. Methods: Ethanol extract from D. microcarpum fruit pulp was analyzed for its antioxidant capacity using ferric reducing antioxidant power...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2017
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ftdoajarticles:oai:doaj.org/article:5718dd98da844b8283df62f69c69244c 2023-05-15T15:09:26+02:00 Antioxidant capacity and genoprotective effect of ethanol fruit extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae) Ablassé Rouamba Maurice Ouedraogo Martin Kiendrebeogo 2017-01-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2016.10.009 https://doaj.org/article/5718dd98da844b8283df62f69c69244c EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169116304725 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.10.009 https://doaj.org/article/5718dd98da844b8283df62f69c69244c Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 1, Pp 32-36 (2017) Antioxidant activity Comet assay Detarium microcarpum Genoprotection Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2017 ftdoajarticles https://doi.org/10.1016/j.apjtb.2016.10.009 2022-12-31T15:28:02Z Objective: To evidence the ability of ethanol fruit extract from Detarium microcarpum (D. microcarpum) to preserve DNA integrity against oxidative genomic damage. Methods: Ethanol extract from D. microcarpum fruit pulp was analyzed for its antioxidant capacity using ferric reducing antioxidant power, 2,2-diphenyl-1-picrylhydrazyl, 2,2′-azinobis-3-ethyl-ethylbenzothiazoline-6-sulphonate, superoxide anion, deoxyribose degradation and lipid peroxidation models. The genoprotective activity was assessed ex vivo by comet assay, on liver cells of NMRI female mice using cyclophosphamide (CP) as genotoxic agent. Results: Ethanol extract from D. microcarpum fruit pulp exhibited interesting antioxidant activity in 2,2-diphenyl-1-picrylhydrazyl, deoxyribose degradation and lipid peroxidation assays. The extract did not present any genotoxic effect but protected DNA against CP-induced damages with a dose-dependent manner. The genoprotective effect observed was related to the antioxidant molecules of the fruit that scavenged the hydroxyl radical (generated by the metabolism of CP) as well as the peroxyl and alkoxyl radicals issued from lipid peroxidation. Other mechanisms such as inactivation of CP metabolism to genotoxic end products, induction of the expression of antioxidant and DNA repair enzymes have been discussed. Conclusions: Our results suggest that the wild edible fruit from D. microcarpum could be beneficial on consumer's health by its antioxidant and genoprotective effects, particularly during chemotherapies exhibiting genotoxic effects like CP in cancer treatment. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 7 1 32 36 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antioxidant activity Comet assay Detarium microcarpum Genoprotection Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Antioxidant activity Comet assay Detarium microcarpum Genoprotection Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Ablassé Rouamba Maurice Ouedraogo Martin Kiendrebeogo Antioxidant capacity and genoprotective effect of ethanol fruit extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae) |
topic_facet |
Antioxidant activity Comet assay Detarium microcarpum Genoprotection Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To evidence the ability of ethanol fruit extract from Detarium microcarpum (D. microcarpum) to preserve DNA integrity against oxidative genomic damage. Methods: Ethanol extract from D. microcarpum fruit pulp was analyzed for its antioxidant capacity using ferric reducing antioxidant power, 2,2-diphenyl-1-picrylhydrazyl, 2,2′-azinobis-3-ethyl-ethylbenzothiazoline-6-sulphonate, superoxide anion, deoxyribose degradation and lipid peroxidation models. The genoprotective activity was assessed ex vivo by comet assay, on liver cells of NMRI female mice using cyclophosphamide (CP) as genotoxic agent. Results: Ethanol extract from D. microcarpum fruit pulp exhibited interesting antioxidant activity in 2,2-diphenyl-1-picrylhydrazyl, deoxyribose degradation and lipid peroxidation assays. The extract did not present any genotoxic effect but protected DNA against CP-induced damages with a dose-dependent manner. The genoprotective effect observed was related to the antioxidant molecules of the fruit that scavenged the hydroxyl radical (generated by the metabolism of CP) as well as the peroxyl and alkoxyl radicals issued from lipid peroxidation. Other mechanisms such as inactivation of CP metabolism to genotoxic end products, induction of the expression of antioxidant and DNA repair enzymes have been discussed. Conclusions: Our results suggest that the wild edible fruit from D. microcarpum could be beneficial on consumer's health by its antioxidant and genoprotective effects, particularly during chemotherapies exhibiting genotoxic effects like CP in cancer treatment. |
format |
Article in Journal/Newspaper |
author |
Ablassé Rouamba Maurice Ouedraogo Martin Kiendrebeogo |
author_facet |
Ablassé Rouamba Maurice Ouedraogo Martin Kiendrebeogo |
author_sort |
Ablassé Rouamba |
title |
Antioxidant capacity and genoprotective effect of ethanol fruit extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae) |
title_short |
Antioxidant capacity and genoprotective effect of ethanol fruit extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae) |
title_full |
Antioxidant capacity and genoprotective effect of ethanol fruit extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae) |
title_fullStr |
Antioxidant capacity and genoprotective effect of ethanol fruit extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae) |
title_full_unstemmed |
Antioxidant capacity and genoprotective effect of ethanol fruit extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae) |
title_sort |
antioxidant capacity and genoprotective effect of ethanol fruit extract from detarium microcarpum guill. and perr. (caesalpiniaceae) |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2017 |
url |
https://doi.org/10.1016/j.apjtb.2016.10.009 https://doaj.org/article/5718dd98da844b8283df62f69c69244c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 1, Pp 32-36 (2017) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169116304725 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.10.009 https://doaj.org/article/5718dd98da844b8283df62f69c69244c |
op_doi |
https://doi.org/10.1016/j.apjtb.2016.10.009 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
7 |
container_issue |
1 |
container_start_page |
32 |
op_container_end_page |
36 |
_version_ |
1766340639106007040 |