P247 and p523: two in vivo-expressed megalocytivirus proteins that induce protective immunity and are essential to viral infection.
Megalocytivirus is a DNA virus with a broad host range among teleost fish. Although the complete genome sequences of a number of megalocytivirus isolates have been reported, the functions of most of the genes of this virus are unknown. In this study, we selected two megalocytivirus immunogens, P247...
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ftdoajarticles:oai:doaj.org/article:56d2ea59fb2f4c0ba7828bf4a786b549 2023-05-15T18:15:52+02:00 P247 and p523: two in vivo-expressed megalocytivirus proteins that induce protective immunity and are essential to viral infection. Jian Zhang Bao Cun Zhang Li Sun 2015-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0121282 https://doaj.org/article/56d2ea59fb2f4c0ba7828bf4a786b549 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4376877?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0121282 https://doaj.org/article/56d2ea59fb2f4c0ba7828bf4a786b549 PLoS ONE, Vol 10, Iss 3, p e0121282 (2015) Medicine R Science Q article 2015 ftdoajarticles https://doi.org/10.1371/journal.pone.0121282 2022-12-31T06:02:45Z Megalocytivirus is a DNA virus with a broad host range among teleost fish. Although the complete genome sequences of a number of megalocytivirus isolates have been reported, the functions of most of the genes of this virus are unknown. In this study, we selected two megalocytivirus immunogens, P247 and P523, which were expressed during host infection and, when in the form of DNA vaccines (pCN247 and pCN523 respectively), elicited strong protectivity against lethal megalocytivirus challenge in a turbot (Scophthalmus maximus) model. Compared to control fish, fish vaccinated with pCN247 and pCN523 exhibited drastically reduced viral loads in tissues and high levels of survival rates. Immune response analysis showed that pCN247 and pCN523 (i) induced production of specific serum antibodies, (ii) caused generation of cytotoxic immune cells and specific memory immune cells that responded to secondary antigen stimulation, and (iii) upregulated the expression of genes involved in innate and adaptive immunity. To examine the potential role of P247 and P523 in viral infection, the expression of P247 and P523 was knocked down by siRNA. Subsequent in vivo infection study showed that P247 and P523 knockdown significantly impaired viral replication. Furthermore, whole-genome transcriptome analysis revealed that P247 and P523 knockdown altered the expression profiles of 26 and 41 viral genes, respectively, putatively participating in diverse aspects of viral infection. Taken together, these results indicate that P247 and P523 induce protective immunity in teleost and play fundamental roles essential to viral replication. These observations provide the first evidence that suggests a likely link between the protectivity of viral immunogens and their biological significance in viral replication. Article in Journal/Newspaper Scophthalmus maximus Turbot Directory of Open Access Journals: DOAJ Articles PLOS ONE 10 3 e0121282 |
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Medicine R Science Q Jian Zhang Bao Cun Zhang Li Sun P247 and p523: two in vivo-expressed megalocytivirus proteins that induce protective immunity and are essential to viral infection. |
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Medicine R Science Q |
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Megalocytivirus is a DNA virus with a broad host range among teleost fish. Although the complete genome sequences of a number of megalocytivirus isolates have been reported, the functions of most of the genes of this virus are unknown. In this study, we selected two megalocytivirus immunogens, P247 and P523, which were expressed during host infection and, when in the form of DNA vaccines (pCN247 and pCN523 respectively), elicited strong protectivity against lethal megalocytivirus challenge in a turbot (Scophthalmus maximus) model. Compared to control fish, fish vaccinated with pCN247 and pCN523 exhibited drastically reduced viral loads in tissues and high levels of survival rates. Immune response analysis showed that pCN247 and pCN523 (i) induced production of specific serum antibodies, (ii) caused generation of cytotoxic immune cells and specific memory immune cells that responded to secondary antigen stimulation, and (iii) upregulated the expression of genes involved in innate and adaptive immunity. To examine the potential role of P247 and P523 in viral infection, the expression of P247 and P523 was knocked down by siRNA. Subsequent in vivo infection study showed that P247 and P523 knockdown significantly impaired viral replication. Furthermore, whole-genome transcriptome analysis revealed that P247 and P523 knockdown altered the expression profiles of 26 and 41 viral genes, respectively, putatively participating in diverse aspects of viral infection. Taken together, these results indicate that P247 and P523 induce protective immunity in teleost and play fundamental roles essential to viral replication. These observations provide the first evidence that suggests a likely link between the protectivity of viral immunogens and their biological significance in viral replication. |
format |
Article in Journal/Newspaper |
author |
Jian Zhang Bao Cun Zhang Li Sun |
author_facet |
Jian Zhang Bao Cun Zhang Li Sun |
author_sort |
Jian Zhang |
title |
P247 and p523: two in vivo-expressed megalocytivirus proteins that induce protective immunity and are essential to viral infection. |
title_short |
P247 and p523: two in vivo-expressed megalocytivirus proteins that induce protective immunity and are essential to viral infection. |
title_full |
P247 and p523: two in vivo-expressed megalocytivirus proteins that induce protective immunity and are essential to viral infection. |
title_fullStr |
P247 and p523: two in vivo-expressed megalocytivirus proteins that induce protective immunity and are essential to viral infection. |
title_full_unstemmed |
P247 and p523: two in vivo-expressed megalocytivirus proteins that induce protective immunity and are essential to viral infection. |
title_sort |
p247 and p523: two in vivo-expressed megalocytivirus proteins that induce protective immunity and are essential to viral infection. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pone.0121282 https://doaj.org/article/56d2ea59fb2f4c0ba7828bf4a786b549 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
PLoS ONE, Vol 10, Iss 3, p e0121282 (2015) |
op_relation |
http://europepmc.org/articles/PMC4376877?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0121282 https://doaj.org/article/56d2ea59fb2f4c0ba7828bf4a786b549 |
op_doi |
https://doi.org/10.1371/journal.pone.0121282 |
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PLOS ONE |
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10 |
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3 |
container_start_page |
e0121282 |
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1766189109622079488 |