Statistical Approach for Production of PUFA from Kocuria sp. BRI 35 Isolated from Marine Water Sample
In this study, Plackett-Burman design was used to identify the most influential parameters affecting PUFA production by Kocuria sp. BRI 35 isolated from Antarctic water sample. Amongst 10 variables evaluated, magnesium chloride, protease peptone, glucose, and temperature were significant. Response s...
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ftdoajarticles:oai:doaj.org/article:568d8b3ef630430388ee4bc718d2cd00 2023-05-15T13:39:16+02:00 Statistical Approach for Production of PUFA from Kocuria sp. BRI 35 Isolated from Marine Water Sample Swanandi Pote Rama Bhadekar 2014-01-01T00:00:00Z https://doi.org/10.1155/2014/570925 https://doaj.org/article/568d8b3ef630430388ee4bc718d2cd00 EN eng Hindawi Limited http://dx.doi.org/10.1155/2014/570925 https://doaj.org/toc/2314-6133 https://doaj.org/toc/2314-6141 2314-6133 2314-6141 doi:10.1155/2014/570925 https://doaj.org/article/568d8b3ef630430388ee4bc718d2cd00 BioMed Research International, Vol 2014 (2014) Medicine R article 2014 ftdoajarticles https://doi.org/10.1155/2014/570925 2022-12-31T09:37:04Z In this study, Plackett-Burman design was used to identify the most influential parameters affecting PUFA production by Kocuria sp. BRI 35 isolated from Antarctic water sample. Amongst 10 variables evaluated, magnesium chloride, protease peptone, glucose, and temperature were significant. Response surface methodology consisting of a central composite design was developed to study the interactions between the variables and to determine optimal values of significant variables. A quadratic model (R = 0.9652, F = 14.64, P < 0.0001) was built. The contour plots indicated that the isolate produced maximum PUFA at lower concentrations of magnesium sulfate (0.9 g/L) and higher concentrations of protease peptone (5 g/L) and glucose (10 g/L) at 15°C. MgSO4 and glucose exhibited quadratic as well as interactive effect on PUFA production whereas protease peptone and temperature showed interactive effects only. After optimization, PUFA production per unit biomass increased from 0.94 mg/g to 11.12 mg/g. This represented an increase from 3% to 58.62% of the total fatty acids. Among PUFAs, the yield of ω-6 fatty acids increased from 9.66 mg/L to 107.71 mg/L with significant increase in linoleic acid (20.36 mg/L) whereas ω-3 fatty acids increased up to 12.37 mg/L with DHA being the major ω-3 fatty acid produced. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic BioMed Research International 2014 1 9 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Medicine R |
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Medicine R Swanandi Pote Rama Bhadekar Statistical Approach for Production of PUFA from Kocuria sp. BRI 35 Isolated from Marine Water Sample |
topic_facet |
Medicine R |
description |
In this study, Plackett-Burman design was used to identify the most influential parameters affecting PUFA production by Kocuria sp. BRI 35 isolated from Antarctic water sample. Amongst 10 variables evaluated, magnesium chloride, protease peptone, glucose, and temperature were significant. Response surface methodology consisting of a central composite design was developed to study the interactions between the variables and to determine optimal values of significant variables. A quadratic model (R = 0.9652, F = 14.64, P < 0.0001) was built. The contour plots indicated that the isolate produced maximum PUFA at lower concentrations of magnesium sulfate (0.9 g/L) and higher concentrations of protease peptone (5 g/L) and glucose (10 g/L) at 15°C. MgSO4 and glucose exhibited quadratic as well as interactive effect on PUFA production whereas protease peptone and temperature showed interactive effects only. After optimization, PUFA production per unit biomass increased from 0.94 mg/g to 11.12 mg/g. This represented an increase from 3% to 58.62% of the total fatty acids. Among PUFAs, the yield of ω-6 fatty acids increased from 9.66 mg/L to 107.71 mg/L with significant increase in linoleic acid (20.36 mg/L) whereas ω-3 fatty acids increased up to 12.37 mg/L with DHA being the major ω-3 fatty acid produced. |
format |
Article in Journal/Newspaper |
author |
Swanandi Pote Rama Bhadekar |
author_facet |
Swanandi Pote Rama Bhadekar |
author_sort |
Swanandi Pote |
title |
Statistical Approach for Production of PUFA from Kocuria sp. BRI 35 Isolated from Marine Water Sample |
title_short |
Statistical Approach for Production of PUFA from Kocuria sp. BRI 35 Isolated from Marine Water Sample |
title_full |
Statistical Approach for Production of PUFA from Kocuria sp. BRI 35 Isolated from Marine Water Sample |
title_fullStr |
Statistical Approach for Production of PUFA from Kocuria sp. BRI 35 Isolated from Marine Water Sample |
title_full_unstemmed |
Statistical Approach for Production of PUFA from Kocuria sp. BRI 35 Isolated from Marine Water Sample |
title_sort |
statistical approach for production of pufa from kocuria sp. bri 35 isolated from marine water sample |
publisher |
Hindawi Limited |
publishDate |
2014 |
url |
https://doi.org/10.1155/2014/570925 https://doaj.org/article/568d8b3ef630430388ee4bc718d2cd00 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
BioMed Research International, Vol 2014 (2014) |
op_relation |
http://dx.doi.org/10.1155/2014/570925 https://doaj.org/toc/2314-6133 https://doaj.org/toc/2314-6141 2314-6133 2314-6141 doi:10.1155/2014/570925 https://doaj.org/article/568d8b3ef630430388ee4bc718d2cd00 |
op_doi |
https://doi.org/10.1155/2014/570925 |
container_title |
BioMed Research International |
container_volume |
2014 |
container_start_page |
1 |
op_container_end_page |
9 |
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1766116654776844288 |