Cocktail of Theileria equi antigens for detecting infection in equines

• Published in: Asian Pacific Journal of Tropical Biomedicine
• Main Authors: Shimaa Abd El-Salam El-Sayed, Mohamed Abdo Rizk, Mohamed Alaa Terkawi, Ahmed Mousa, El Said El Shirbini El Said, Gehad Elsayed, Mohamed Fouda, Naoaki Yokoyama, Ikuo Igarashi
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wolters Kluwer Medknow Publications 2015
• Subjects: Theileria equi; Equi merozoite antigen-2; Theileria equi protein 82; Theileria equi protein 43; Cocktail formula; Arctic medicine. Tropical medicine; RC955-962; Biology (General); QH301-705.5; Arctic
• Online Access: https://doi.org/10.1016/j.apjtb.2015.09.001; https://doaj.org/article/567f071d6bff4077a12bf7f8ced79bfa

Objective: To use two diagnostic antigens belonging to the frequently associated in Theileria domain, Theileria equi (T. equi) protein 82 (Te 82) and T. equi 104 kDa microneme-rhoptry antigen precursor (Te 43), to diagnose T. equi infection in horses as compared with equi merozoite antigen-2 (EMA-2). Methods: In the current study, we applied a cocktail-ELISA containing two antigens (EMA-2 + Te 82) to diagnose T. equi infection either in experimentally infected horses or in field infection. Results: Our findings have revealed that a cocktail formula of EMA-2 + Te 82 provided a more practical and sensitive diagnostic candidate for diagnosing T. equi infection in horses as compared with Te 82 or Te 43 alone. Conclusions: The ELISA technique using a cocktail formula of EMA-2 + Te 82 offers a practical and sensitive diagnostic tool for diagnosing T. equi infection in horses and using of this promising cocktail formula will be applicable for epidemiological surveys and will help control the infection in horses.