Development and Application of an ELISA Assay Using Excretion/Secretion Proteins from Epimastigote Forms of T. cruzi (ESEA Antigens) for the Diagnosis of Chagas Disease
An indirect enzyme-linked immunoabsorbent assay (ELISA) for Trypanosoma cruzi was developed using epimastigote secretion/excretion proteins (ESEA antigens) obtained from axenic culture supernatants. A panel of 120 serum samples from subjects with confirmed Chagas disease (n=50), healthy controls (n=...
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ftdoajarticles:oai:doaj.org/article:563e2312b9a04c27acaa48598da01281 2024-09-09T19:25:20+00:00 Development and Application of an ELISA Assay Using Excretion/Secretion Proteins from Epimastigote Forms of T. cruzi (ESEA Antigens) for the Diagnosis of Chagas Disease Mariolga Berrizbeitia Milagros Figueroa Brian J. Ward Jessicca Rodríguez Alicia Jorquera Maria A. Figuera Leomerys Romero Momar Ndao 2012-01-01T00:00:00Z https://doi.org/10.1155/2012/875909 https://doaj.org/article/563e2312b9a04c27acaa48598da01281 EN eng Wiley http://dx.doi.org/10.1155/2012/875909 https://doaj.org/toc/1687-9686 https://doaj.org/toc/1687-9694 1687-9686 1687-9694 doi:10.1155/2012/875909 https://doaj.org/article/563e2312b9a04c27acaa48598da01281 Journal of Tropical Medicine, Vol 2012 (2012) Arctic medicine. Tropical medicine RC955-962 article 2012 ftdoajarticles https://doi.org/10.1155/2012/875909 2024-08-05T17:48:35Z An indirect enzyme-linked immunoabsorbent assay (ELISA) for Trypanosoma cruzi was developed using epimastigote secretion/excretion proteins (ESEA antigens) obtained from axenic culture supernatants. A panel of 120 serum samples from subjects with confirmed Chagas disease (n=50), healthy controls (n=50), and patients with other parasitic diseases (n=20) was used to evaluate the new ESEA-based ELISA (ELISAESEA). This new test had excellent sensitivity (98%) and acceptable specificity (88%). Cross-reactivity was observed largely in sera from subjects with Leishmania and Ascaris infections. Using Western blotting and epimastigotes from two distinct T. cruzi isolates, several polypeptide bands with molecular masses ranging from 50 to 220 kDa were detected in pooled chagasic sera. However, the band pattern for each isolate was different. These data suggest that an inexpensive and technically simple ELISA based on ESEA antigens is a promising new tool for the diagnosis of Chagas disease. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Tropical Medicine 2012 1 7 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Mariolga Berrizbeitia Milagros Figueroa Brian J. Ward Jessicca Rodríguez Alicia Jorquera Maria A. Figuera Leomerys Romero Momar Ndao Development and Application of an ELISA Assay Using Excretion/Secretion Proteins from Epimastigote Forms of T. cruzi (ESEA Antigens) for the Diagnosis of Chagas Disease |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 |
description |
An indirect enzyme-linked immunoabsorbent assay (ELISA) for Trypanosoma cruzi was developed using epimastigote secretion/excretion proteins (ESEA antigens) obtained from axenic culture supernatants. A panel of 120 serum samples from subjects with confirmed Chagas disease (n=50), healthy controls (n=50), and patients with other parasitic diseases (n=20) was used to evaluate the new ESEA-based ELISA (ELISAESEA). This new test had excellent sensitivity (98%) and acceptable specificity (88%). Cross-reactivity was observed largely in sera from subjects with Leishmania and Ascaris infections. Using Western blotting and epimastigotes from two distinct T. cruzi isolates, several polypeptide bands with molecular masses ranging from 50 to 220 kDa were detected in pooled chagasic sera. However, the band pattern for each isolate was different. These data suggest that an inexpensive and technically simple ELISA based on ESEA antigens is a promising new tool for the diagnosis of Chagas disease. |
format |
Article in Journal/Newspaper |
author |
Mariolga Berrizbeitia Milagros Figueroa Brian J. Ward Jessicca Rodríguez Alicia Jorquera Maria A. Figuera Leomerys Romero Momar Ndao |
author_facet |
Mariolga Berrizbeitia Milagros Figueroa Brian J. Ward Jessicca Rodríguez Alicia Jorquera Maria A. Figuera Leomerys Romero Momar Ndao |
author_sort |
Mariolga Berrizbeitia |
title |
Development and Application of an ELISA Assay Using Excretion/Secretion Proteins from Epimastigote Forms of T. cruzi (ESEA Antigens) for the Diagnosis of Chagas Disease |
title_short |
Development and Application of an ELISA Assay Using Excretion/Secretion Proteins from Epimastigote Forms of T. cruzi (ESEA Antigens) for the Diagnosis of Chagas Disease |
title_full |
Development and Application of an ELISA Assay Using Excretion/Secretion Proteins from Epimastigote Forms of T. cruzi (ESEA Antigens) for the Diagnosis of Chagas Disease |
title_fullStr |
Development and Application of an ELISA Assay Using Excretion/Secretion Proteins from Epimastigote Forms of T. cruzi (ESEA Antigens) for the Diagnosis of Chagas Disease |
title_full_unstemmed |
Development and Application of an ELISA Assay Using Excretion/Secretion Proteins from Epimastigote Forms of T. cruzi (ESEA Antigens) for the Diagnosis of Chagas Disease |
title_sort |
development and application of an elisa assay using excretion/secretion proteins from epimastigote forms of t. cruzi (esea antigens) for the diagnosis of chagas disease |
publisher |
Wiley |
publishDate |
2012 |
url |
https://doi.org/10.1155/2012/875909 https://doaj.org/article/563e2312b9a04c27acaa48598da01281 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Tropical Medicine, Vol 2012 (2012) |
op_relation |
http://dx.doi.org/10.1155/2012/875909 https://doaj.org/toc/1687-9686 https://doaj.org/toc/1687-9694 1687-9686 1687-9694 doi:10.1155/2012/875909 https://doaj.org/article/563e2312b9a04c27acaa48598da01281 |
op_doi |
https://doi.org/10.1155/2012/875909 |
container_title |
Journal of Tropical Medicine |
container_volume |
2012 |
container_start_page |
1 |
op_container_end_page |
7 |
_version_ |
1809895069765861376 |